IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals
ABSTRACT Objective This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods...
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| Format: | Article |
| Language: | English |
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Brazilian Society of Endocrinology and Metabolism
2017-02-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000500438&lng=en&tlng=en |
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| author | Kathryna Fontana Rodrigues Nathalia Teixeira Pietrani Adriana Aparecida Bosco Fernanda Magalhães Freire Campos Valéria Cristina Sandrim Karina Braga Gomes |
| author_facet | Kathryna Fontana Rodrigues Nathalia Teixeira Pietrani Adriana Aparecida Bosco Fernanda Magalhães Freire Campos Valéria Cristina Sandrim Karina Braga Gomes |
| author_sort | Kathryna Fontana Rodrigues |
| collection | DOAJ |
| description | ABSTRACT Objective This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease. |
| first_indexed | 2024-12-12T08:43:47Z |
| format | Article |
| id | doaj.art-f6992201b69749f3bd46ecee48854db8 |
| institution | Directory Open Access Journal |
| issn | 2359-4292 |
| language | English |
| last_indexed | 2024-12-12T08:43:47Z |
| publishDate | 2017-02-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj.art-f6992201b69749f3bd46ecee48854db82022-12-22T00:30:41ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922017-02-0161543844610.1590/2359-3997000000254S2359-39972017000500438IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individualsKathryna Fontana RodriguesNathalia Teixeira PietraniAdriana Aparecida BoscoFernanda Magalhães Freire CamposValéria Cristina SandrimKarina Braga GomesABSTRACT Objective This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. Subjects and methods One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. Results IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. Conclusions These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000500438&lng=en&tlng=enType 2 diabetes mellituspolymorphismsinterleukin-6interleukin-10tumor necrosis factor-alpha |
| spellingShingle | Kathryna Fontana Rodrigues Nathalia Teixeira Pietrani Adriana Aparecida Bosco Fernanda Magalhães Freire Campos Valéria Cristina Sandrim Karina Braga Gomes IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals Archives of Endocrinology and Metabolism Type 2 diabetes mellitus polymorphisms interleukin-6 interleukin-10 tumor necrosis factor-alpha |
| title | IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
| title_full | IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
| title_fullStr | IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
| title_full_unstemmed | IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
| title_short | IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals |
| title_sort | il 6 tnf α and il 10 levels polymorphisms and their association with type 2 diabetes mellitus and obesity in brazilian individuals |
| topic | Type 2 diabetes mellitus polymorphisms interleukin-6 interleukin-10 tumor necrosis factor-alpha |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000500438&lng=en&tlng=en |
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