Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS
Cancer is mostly incurable when diagnosed at a metastatic stage, making its early detection via blood proteins of immense clinical interest. Proteomic changes in tumor tissue may lead to changes detectable in the protein composition of circulating blood plasma. Using a proteomic workflow combining N...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718307149 |
| _version_ | 1828975118109376512 |
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| author | Tatjana Sajic Yansheng Liu Eirini Arvaniti Silvia Surinova Evan G. Williams Ralph Schiess Ruth Hüttenhain Atul Sethi Sheng Pan Teresa A. Brentnall Ru Chen Peter Blattmann Betty Friedrich Emma Niméus Susanne Malander Aurelius Omlin Silke Gillessen Manfred Claassen Ruedi Aebersold |
| author_facet | Tatjana Sajic Yansheng Liu Eirini Arvaniti Silvia Surinova Evan G. Williams Ralph Schiess Ruth Hüttenhain Atul Sethi Sheng Pan Teresa A. Brentnall Ru Chen Peter Blattmann Betty Friedrich Emma Niméus Susanne Malander Aurelius Omlin Silke Gillessen Manfred Claassen Ruedi Aebersold |
| author_sort | Tatjana Sajic |
| collection | DOAJ |
| description | Cancer is mostly incurable when diagnosed at a metastatic stage, making its early detection via blood proteins of immense clinical interest. Proteomic changes in tumor tissue may lead to changes detectable in the protein composition of circulating blood plasma. Using a proteomic workflow combining N-glycosite enrichment and SWATH mass spectrometry, we generate a data resource of 284 blood samples derived from patients with different types of localized-stage carcinomas and from matched controls. We observe whether the changes in the patient’s plasma are specific to a particular carcinoma or represent a generic signature of proteins modified uniformly in a common, systemic response to many cancers. A quantitative comparison of the resulting N-glycosite profiles discovers that proteins related to blood platelets are common to several cancers (e.g., THBS1), whereas others are highly cancer-type specific. Available proteomics data, including a SWATH library to study N-glycoproteins, will facilitate follow-up biomarker research into early cancer detection. |
| first_indexed | 2024-12-14T14:15:24Z |
| format | Article |
| id | doaj.art-f69a33aae6bb4289a7cf96673277a7c8 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-14T14:15:24Z |
| publishDate | 2018-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f69a33aae6bb4289a7cf96673277a7c82022-12-21T22:58:13ZengElsevierCell Reports2211-12472018-05-0123928192831.e510.1016/j.celrep.2018.04.114Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MSTatjana Sajic0Yansheng Liu1Eirini Arvaniti2Silvia Surinova3Evan G. Williams4Ralph Schiess5Ruth Hüttenhain6Atul Sethi7Sheng Pan8Teresa A. Brentnall9Ru Chen10Peter Blattmann11Betty Friedrich12Emma Niméus13Susanne Malander14Aurelius Omlin15Silke Gillessen16Manfred Claassen17Ruedi Aebersold18Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandDepartment of Pharmacology, Cancer Biology Institute, Yale University School of Medicine, West Haven, CT 06516, USADepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandUCL Cancer Institute, University College London, London, UKDepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandProteoMediX AG, 8952 Schlieren, SwitzerlandDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USADepartment of Biomedicine, University of Basel/University Hospital Basel, and Swiss Institute of Bioinformatics, Basel, SwitzerlandThe Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, 1825 Pressler, Houston, TX 77030, USADepartment of Medicine, University of Washington, Seattle, WA 98195, USADepartment of Medicine, University of Washington, Seattle, WA 98195, USADepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandDepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandDepartment of Clinical Sciences Lund, Surgery, Oncology and Pathology, Lund University, and Skåne University Hospital, Department of Surgery, Lund, SwedenDepartment of Clinical Sciences Lund, Oncology and Pathology, Lund University, and Skåne University Hospital, Department of Oncology, Lund, SwedenDepartment of Oncology and Hematology, Kantonsspital St. Gallen, St. Gallen, SwitzerlandDepartment of Oncology and Hematology, Kantonsspital St. Gallen, St. Gallen, SwitzerlandDepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandDepartment of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, SwitzerlandCancer is mostly incurable when diagnosed at a metastatic stage, making its early detection via blood proteins of immense clinical interest. Proteomic changes in tumor tissue may lead to changes detectable in the protein composition of circulating blood plasma. Using a proteomic workflow combining N-glycosite enrichment and SWATH mass spectrometry, we generate a data resource of 284 blood samples derived from patients with different types of localized-stage carcinomas and from matched controls. We observe whether the changes in the patient’s plasma are specific to a particular carcinoma or represent a generic signature of proteins modified uniformly in a common, systemic response to many cancers. A quantitative comparison of the resulting N-glycosite profiles discovers that proteins related to blood platelets are common to several cancers (e.g., THBS1), whereas others are highly cancer-type specific. Available proteomics data, including a SWATH library to study N-glycoproteins, will facilitate follow-up biomarker research into early cancer detection.http://www.sciencedirect.com/science/article/pii/S2211124718307149carcinomalocalized cancerbloodglycoproteinssecretedSWATH-mass spectrometryhuman clinical study |
| spellingShingle | Tatjana Sajic Yansheng Liu Eirini Arvaniti Silvia Surinova Evan G. Williams Ralph Schiess Ruth Hüttenhain Atul Sethi Sheng Pan Teresa A. Brentnall Ru Chen Peter Blattmann Betty Friedrich Emma Niméus Susanne Malander Aurelius Omlin Silke Gillessen Manfred Claassen Ruedi Aebersold Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS Cell Reports carcinoma localized cancer blood glycoproteins secreted SWATH-mass spectrometry human clinical study |
| title | Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS |
| title_full | Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS |
| title_fullStr | Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS |
| title_full_unstemmed | Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS |
| title_short | Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS |
| title_sort | similarities and differences of blood n glycoproteins in five solid carcinomas at localized clinical stage analyzed by swath ms |
| topic | carcinoma localized cancer blood glycoproteins secreted SWATH-mass spectrometry human clinical study |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718307149 |
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