Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study
Background: Colorectal carcinogenesis has been found to be associated with the polymorphic status of Toll-like receptor 4 gene in various populations of the world. Aim: The aim of the study was to determine the genetic association of TLR4 gene polymorphisms (Asp299Gly and Thr399Ile) with disease sus...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-01-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S111086301630009X |
| _version_ | 1811286589501865984 |
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| author | Saniya Nissar Aga Syed Sameer Roohi Rasool Qurteeba Qadri Nissar A. Chowdri Fouzia Rashid |
| author_facet | Saniya Nissar Aga Syed Sameer Roohi Rasool Qurteeba Qadri Nissar A. Chowdri Fouzia Rashid |
| author_sort | Saniya Nissar |
| collection | DOAJ |
| description | Background: Colorectal carcinogenesis has been found to be associated with the polymorphic status of Toll-like receptor 4 gene in various populations of the world.
Aim: The aim of the study was to determine the genetic association of TLR4 gene polymorphisms (Asp299Gly and Thr399Ile) with disease susceptibility and risk development in colorectal cancer (CRC) patients of Kashmir, India.
Materials and methods: Genotype frequencies of TLR4 polymorphisms (Asp299Gly and Thr399Ile) were compared between 120 CRC patients and 200 healthy controls using PCR-RFLP method.
Results: We did not find any significant association between the TLR4 gene polymorphisms and the CRC cases (p > 0.05). However CT genotype (Thr399Ile) showed modest elevated risk of the development of CRC [OR = 1.78 95% CI (0.88–3.5)]. Also G allele (AG genotype) of TLR-4 Asp299Gly polymorphism was found to be significantly associated with the male gender (p value = 0.006) and involvement of Nodes (p value = 0.01) whereas, T allele (CT genotype) of Thr399Ile polymorphism showed significant association with the smoking status (p value = 0.03).
Conclusion: Our results suggest that TLR4 gene polymorphism is not a key modulator of the risk of developing colorectal cancer in Kashmiri population. |
| first_indexed | 2024-04-13T03:02:37Z |
| format | Article |
| id | doaj.art-f69d7bdfcef744c6bb56924a72873744 |
| institution | Directory Open Access Journal |
| issn | 1110-8630 |
| language | English |
| last_indexed | 2024-04-13T03:02:37Z |
| publishDate | 2017-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-f69d7bdfcef744c6bb56924a728737442022-12-22T03:05:22ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302017-01-01181535910.1016/j.ejmhg.2016.04.004Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control studySaniya Nissar0Aga Syed Sameer1Roohi Rasool2Qurteeba Qadri3Nissar A. Chowdri4Fouzia Rashid5Department of Biochemistry, University of Kashmir, Hazratbal, Srinagar, Kashmir, IndiaDepartment of Basic Medical Sciences, College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Kingdom of Saudi ArabiaDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, IndiaDepartment of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, IndiaDepartment of Surgery, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, IndiaDepartment of Clinical Biochemistry, University of Kashmir, Hazratbal, Srinagar, Kashmir, IndiaBackground: Colorectal carcinogenesis has been found to be associated with the polymorphic status of Toll-like receptor 4 gene in various populations of the world. Aim: The aim of the study was to determine the genetic association of TLR4 gene polymorphisms (Asp299Gly and Thr399Ile) with disease susceptibility and risk development in colorectal cancer (CRC) patients of Kashmir, India. Materials and methods: Genotype frequencies of TLR4 polymorphisms (Asp299Gly and Thr399Ile) were compared between 120 CRC patients and 200 healthy controls using PCR-RFLP method. Results: We did not find any significant association between the TLR4 gene polymorphisms and the CRC cases (p > 0.05). However CT genotype (Thr399Ile) showed modest elevated risk of the development of CRC [OR = 1.78 95% CI (0.88–3.5)]. Also G allele (AG genotype) of TLR-4 Asp299Gly polymorphism was found to be significantly associated with the male gender (p value = 0.006) and involvement of Nodes (p value = 0.01) whereas, T allele (CT genotype) of Thr399Ile polymorphism showed significant association with the smoking status (p value = 0.03). Conclusion: Our results suggest that TLR4 gene polymorphism is not a key modulator of the risk of developing colorectal cancer in Kashmiri population.http://www.sciencedirect.com/science/article/pii/S111086301630009XColorectal cancerKashmirPolymorphismRFLPTLR4 |
| spellingShingle | Saniya Nissar Aga Syed Sameer Roohi Rasool Qurteeba Qadri Nissar A. Chowdri Fouzia Rashid Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study Egyptian Journal of Medical Human Genetics Colorectal cancer Kashmir Polymorphism RFLP TLR4 |
| title | Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study |
| title_full | Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study |
| title_fullStr | Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study |
| title_full_unstemmed | Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study |
| title_short | Role of TLR4 gene polymorphisms in the colorectal cancer risk modulation in ethnic Kashmiri population – A case–control study |
| title_sort | role of tlr4 gene polymorphisms in the colorectal cancer risk modulation in ethnic kashmiri population a case control study |
| topic | Colorectal cancer Kashmir Polymorphism RFLP TLR4 |
| url | http://www.sciencedirect.com/science/article/pii/S111086301630009X |
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