Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish
Zebrafish (Danio rerio) have emerged as a popular model for studying the pharmacology and behavior of anxiety. While there have been numerous studies documenting the anxiolytic and anxiogenic effects of common drugs in zebrafish, many do not report or test for behavioral differences between the sexe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2016-08-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/2352.pdf |
| _version_ | 1827608284280913920 |
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| author | Matthew L. Singer Kris Oreschak Zachariah Rhinehart Barrie D. Robison |
| author_facet | Matthew L. Singer Kris Oreschak Zachariah Rhinehart Barrie D. Robison |
| author_sort | Matthew L. Singer |
| collection | DOAJ |
| description | Zebrafish (Danio rerio) have emerged as a popular model for studying the pharmacology and behavior of anxiety. While there have been numerous studies documenting the anxiolytic and anxiogenic effects of common drugs in zebrafish, many do not report or test for behavioral differences between the sexes. Previous studies have indicated that males and females differ in their baseline level of anxiety. In this study, we test for a sex interaction with fluoxetine and nicotine. We exposed fish to system water (control), 10 mg/L fluoxetine, or 1 mg/L nicotine for three minutes prior to being subjected to four minutes in an open-field drop test. Video recordings were tracked using ProAnalyst. Fish from both drug treatments reduced swimming speed, increased vertical position, and increased use of the top half of the open field when compared with the control, though fluoxetine had a larger effect on depth related behaviors while nicotine mostly affected swimming speed. A significant sex effect was observed where females swam at a slower and more constant speed than males, however neither drug produced a sex-dependent response. |
| first_indexed | 2024-03-09T07:08:54Z |
| format | Article |
| id | doaj.art-f6a0af96ef184ddaabe965263581890f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:08:54Z |
| publishDate | 2016-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-f6a0af96ef184ddaabe965263581890f2023-12-03T09:21:05ZengPeerJ Inc.PeerJ2167-83592016-08-014e235210.7717/peerj.2352Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafishMatthew L. Singer0Kris Oreschak1Zachariah Rhinehart2Barrie D. Robison3Department of Biological Sciences, University of Idaho, Moscow, ID, United StatesDepartment of Biological Sciences, University of Idaho, Moscow, ID, United StatesDepartment of Biological Sciences, University of Idaho, Moscow, ID, United StatesDepartment of Biological Sciences, University of Idaho, Moscow, ID, United StatesZebrafish (Danio rerio) have emerged as a popular model for studying the pharmacology and behavior of anxiety. While there have been numerous studies documenting the anxiolytic and anxiogenic effects of common drugs in zebrafish, many do not report or test for behavioral differences between the sexes. Previous studies have indicated that males and females differ in their baseline level of anxiety. In this study, we test for a sex interaction with fluoxetine and nicotine. We exposed fish to system water (control), 10 mg/L fluoxetine, or 1 mg/L nicotine for three minutes prior to being subjected to four minutes in an open-field drop test. Video recordings were tracked using ProAnalyst. Fish from both drug treatments reduced swimming speed, increased vertical position, and increased use of the top half of the open field when compared with the control, though fluoxetine had a larger effect on depth related behaviors while nicotine mostly affected swimming speed. A significant sex effect was observed where females swam at a slower and more constant speed than males, however neither drug produced a sex-dependent response.https://peerj.com/articles/2352.pdfBehaviorAnxietyFluoxetineNicotine |
| spellingShingle | Matthew L. Singer Kris Oreschak Zachariah Rhinehart Barrie D. Robison Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish PeerJ Behavior Anxiety Fluoxetine Nicotine |
| title | Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| title_full | Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| title_fullStr | Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| title_full_unstemmed | Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| title_short | Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| title_sort | anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish |
| topic | Behavior Anxiety Fluoxetine Nicotine |
| url | https://peerj.com/articles/2352.pdf |
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