Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.)
Presence of high amount of polysaccharide and polyphenolic compounds in barnacles restricts the isolation of high molecular weight good quality DNA. CTAB, which is a detergent used to isolate DNA from high polysaccharide containing plants, is used in this study to extract quality DNA from ship barna...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-06-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/91147 |
| _version_ | 1797896715935678464 |
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| author | ARCHANA MISHRA UTSA ROY SIMRAN KRISHNAKANT KUSHWAHA SUHASINI LULLA REETI PRAJAPATI |
| author_facet | ARCHANA MISHRA UTSA ROY SIMRAN KRISHNAKANT KUSHWAHA SUHASINI LULLA REETI PRAJAPATI |
| author_sort | ARCHANA MISHRA |
| collection | DOAJ |
| description | Presence of high amount of polysaccharide and polyphenolic compounds in barnacles restricts the isolation of high molecular weight good quality DNA. CTAB, which is a detergent used to isolate DNA from high polysaccharide containing plants, is used in this study to extract quality DNA from ship barnacle. This modified CTAB method is simple, efficient and quick enough to be carried out within 2 h. Taking DNAzol ® isolated DNA as a reference, restriction digestion and PCR was performed to prove the absence of any enzyme inhibitory substances in the isolate. Traditional phenol-chloroform and salting out method failed to even isolate the DNA from barnacle sample. The molecular weight of the modified CTAB extracted DNA was higher than that of DNAzol ® extracted DNA which makes it better candidate for analyses like whole genome sequencing. |
| first_indexed | 2024-04-10T07:47:12Z |
| format | Article |
| id | doaj.art-f6a2df8f928a4e17b604e0acaf519ba4 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:47:12Z |
| publishDate | 2019-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-f6a2df8f928a4e17b604e0acaf519ba42023-02-23T10:32:05ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-06-0189610.56093/ijans.v89i6.91147Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.)ARCHANA MISHRA0UTSA ROY1SIMRAN KRISHNAKANT KUSHWAHA2SUHASINI LULLA3REETI PRAJAPATI4Senior Research Fellow, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 IndiaPhD Scholar, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 IndiaScholar, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 IndiaScholar, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 IndiaScholar, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 IndiaPresence of high amount of polysaccharide and polyphenolic compounds in barnacles restricts the isolation of high molecular weight good quality DNA. CTAB, which is a detergent used to isolate DNA from high polysaccharide containing plants, is used in this study to extract quality DNA from ship barnacle. This modified CTAB method is simple, efficient and quick enough to be carried out within 2 h. Taking DNAzol ® isolated DNA as a reference, restriction digestion and PCR was performed to prove the absence of any enzyme inhibitory substances in the isolate. Traditional phenol-chloroform and salting out method failed to even isolate the DNA from barnacle sample. The molecular weight of the modified CTAB extracted DNA was higher than that of DNAzol ® extracted DNA which makes it better candidate for analyses like whole genome sequencing.https://epubs.icar.org.in/index.php/IJAnS/article/view/91147BarnacleDNA extractionDNAzolHigh-quality DNAModified CTAB |
| spellingShingle | ARCHANA MISHRA UTSA ROY SIMRAN KRISHNAKANT KUSHWAHA SUHASINI LULLA REETI PRAJAPATI Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) Indian Journal of Animal Sciences Barnacle DNA extraction DNAzol High-quality DNA Modified CTAB |
| title | Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) |
| title_full | Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) |
| title_fullStr | Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) |
| title_full_unstemmed | Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) |
| title_short | Modified CTAB method for high-quality genomic DNA extraction from ship barnacle (Balanus sp.) |
| title_sort | modified ctab method for high quality genomic dna extraction from ship barnacle balanus sp |
| topic | Barnacle DNA extraction DNAzol High-quality DNA Modified CTAB |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/91147 |
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