Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose
Objective: The protective effect of Polygonatum odoratum polysaccharides (POP) on D-galactose (D-gal) induced senescence in rat aortic smooth muscle cells (A7r5) was investigated. Methods: Firstly, the POP was extracted by the water extraction and alcohol precipitation methods. Next, the phenol conc...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070322 |
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| author | Minghui LI Junyu HOU Di HU Jing LIU Mengdan ZHAO Junhong GE Ye SHEN Tan LI Yubo PENG Peng LIU Xin SUN |
| author_facet | Minghui LI Junyu HOU Di HU Jing LIU Mengdan ZHAO Junhong GE Ye SHEN Tan LI Yubo PENG Peng LIU Xin SUN |
| author_sort | Minghui LI |
| collection | DOAJ |
| description | Objective: The protective effect of Polygonatum odoratum polysaccharides (POP) on D-galactose (D-gal) induced senescence in rat aortic smooth muscle cells (A7r5) was investigated. Methods: Firstly, the POP was extracted by the water extraction and alcohol precipitation methods. Next, the phenol concentrated sulfuric acid method was applied to further measure the total sugar content of POP. In addition, the A7r5-senescent cell model was established by the D-galactose treatment. Finally, the MTT assay, β-galactosidase staining, reactive oxygen species (ROS) staining as well as JC-1 staining were applied to further detect the protective effect of POP in A7r5-senescent cells induced by D-galactose. Results: This study found an yield of POP was 8.23% and its total sugar content was 76.48%±0.036%. As a result, the MTT assay showed the viability of the A7r5 cells was increased to 174.89%±3.30% after 24 h treatment by 400 μg/mL of POP, while it was decreased to 65.93%±1.63% after 48 h treatment by 40 mg/mL of D-galactose. Interestingly, when a concentration of POP was 100 μg/mL, this study had found the most significant protective effect in A7r5-senescent cells induced by D-galactose, which increased its cell viability to 226.87%±12.58% as compared with the control group (P<0.001). Additionally, POP decreased the total β-galactosidase, inhibited its ROS levels, increased the number of positive cells after JC-1 staining, as well as protected the stability of mitochondrial membrane potential in A7r5 cells induced by D-galactose. Conclusion: POP had a potential role in the suppression of oxidative stress and cellular senescence in A7r5 cells induced by D-galactose, which could further support its anti-aging effects in vitro. |
| first_indexed | 2024-04-13T09:08:25Z |
| format | Article |
| id | doaj.art-f6a605df15914359936c2455c0a8b697 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:08:25Z |
| publishDate | 2022-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-f6a605df15914359936c2455c0a8b6972022-12-22T02:52:56ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-03-0143638038810.13386/j.issn1002-0306.20210703222021070322-6Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-GalactoseMinghui LI0Junyu HOU1Di HU2Jing LIU3Mengdan ZHAO4Junhong GE5Ye SHEN6Tan LI7Yubo PENG8Peng LIU9Xin SUN10College of Basic Medicine, Beihua University, Jilin 132013, ChinaCollege of Basic Medicine, Beihua University, Jilin 132013, ChinaCollege of Basic Medicine, Beihua University, Jilin 132013, ChinaCollege of Basic Medicine, Beihua University, Jilin 132013, ChinaCollege of Basic Medicine, Beihua University, Jilin 132013, ChinaCollege of Pharmacy, Beihua University, Jilin 132013, ChinaCollege of Public Health, Beihua University, Jilin 132013, ChinaCollege of Public Health, Beihua University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical College, Jilin 132013, ChinaCollege of Stomatology, Beihua University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical College, Jilin 132013, ChinaObjective: The protective effect of Polygonatum odoratum polysaccharides (POP) on D-galactose (D-gal) induced senescence in rat aortic smooth muscle cells (A7r5) was investigated. Methods: Firstly, the POP was extracted by the water extraction and alcohol precipitation methods. Next, the phenol concentrated sulfuric acid method was applied to further measure the total sugar content of POP. In addition, the A7r5-senescent cell model was established by the D-galactose treatment. Finally, the MTT assay, β-galactosidase staining, reactive oxygen species (ROS) staining as well as JC-1 staining were applied to further detect the protective effect of POP in A7r5-senescent cells induced by D-galactose. Results: This study found an yield of POP was 8.23% and its total sugar content was 76.48%±0.036%. As a result, the MTT assay showed the viability of the A7r5 cells was increased to 174.89%±3.30% after 24 h treatment by 400 μg/mL of POP, while it was decreased to 65.93%±1.63% after 48 h treatment by 40 mg/mL of D-galactose. Interestingly, when a concentration of POP was 100 μg/mL, this study had found the most significant protective effect in A7r5-senescent cells induced by D-galactose, which increased its cell viability to 226.87%±12.58% as compared with the control group (P<0.001). Additionally, POP decreased the total β-galactosidase, inhibited its ROS levels, increased the number of positive cells after JC-1 staining, as well as protected the stability of mitochondrial membrane potential in A7r5 cells induced by D-galactose. Conclusion: POP had a potential role in the suppression of oxidative stress and cellular senescence in A7r5 cells induced by D-galactose, which could further support its anti-aging effects in vitro.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070322polygonatum odoratum polysaccharidecell senescenceβ-galactosidasereactive oxygen speciesmitochondrial membrane potential |
| spellingShingle | Minghui LI Junyu HOU Di HU Jing LIU Mengdan ZHAO Junhong GE Ye SHEN Tan LI Yubo PENG Peng LIU Xin SUN Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose Shipin gongye ke-ji polygonatum odoratum polysaccharide cell senescence β-galactosidase reactive oxygen species mitochondrial membrane potential |
| title | Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
| title_full | Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
| title_fullStr | Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
| title_full_unstemmed | Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
| title_short | Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose |
| title_sort | protective effect of polygonatum odoratum polysaccharides on a7r5 cell senescence induced by d galactose |
| topic | polygonatum odoratum polysaccharide cell senescence β-galactosidase reactive oxygen species mitochondrial membrane potential |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070322 |
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