Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections
The objectives of this study were to report on the antimicrobial susceptibility of 141 clinically significant anaerobic bacteria isolated from bloodstream infections between January 2016 and April 2020 in a tertiary-care hospital in Granada (Spain) and to describe the main clinical features of the p...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/6/345 |
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| author | Fernando Cobo Jaime Borrego Esther Gómez Isabel Casanovas Elizabeth Calatrava Carla Foronda José María Navarro-Marí |
| author_facet | Fernando Cobo Jaime Borrego Esther Gómez Isabel Casanovas Elizabeth Calatrava Carla Foronda José María Navarro-Marí |
| author_sort | Fernando Cobo |
| collection | DOAJ |
| description | The objectives of this study were to report on the antimicrobial susceptibility of 141 clinically significant anaerobic bacteria isolated from bloodstream infections between January 2016 and April 2020 in a tertiary-care hospital in Granada (Spain) and to describe the main clinical features of the patients. Species identification was performed by MALDI-TOF MS (Bruker Daltonics, Billerica, MA, USA). Antimicrobial susceptibility tests were performed against penicillin, amoxicillin-clavulanic acid, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin-tazobactam using the gradient diffusion technique and EUCAST breakpoints, except for moxifloxacin (CLSI breakpoints). The most frequent anaerobes were <i>Bacteroides</i> (43.9%, <i>n</i> = 62), <i>Clostridium</i> (24.1%, <i>n</i> = 34) and Gram-positive anaerobic cocci (GPACs) (15.6%, <i>n</i> = 22). Almost all tested anaerobes were susceptible to imipenem and amoxicillin-clavulanic acid, except for <i>Bacteroides</i>. High overall resistance rates to clindamycin were observed, especially for Gram-positive anaerobic cocci (GPACs) (54.5%) and for <i>Bacteroides</i> spp. (45.1%). Overall, low resistance rates to almost all antibiotics were observed for <i>Clostridium</i>. High resistance rates to penicillin were also observed for Gram-positive anaerobic bacilli (GPABs) (44.4%), as well as to metronidazole (22.2%), although only nine isolates were included. Antimicrobial susceptibility testing for anaerobes should always be performed in severe infections, such as those localized in the bloodstream. The information obtained contributes to selecting empirical treatments according with local data on resistance. |
| first_indexed | 2024-03-10T19:02:46Z |
| format | Article |
| id | doaj.art-f6a69e3616d7449a9c5d72b530fcccdb |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T19:02:46Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-f6a69e3616d7449a9c5d72b530fcccdb2023-11-20T04:19:29ZengMDPI AGAntibiotics2079-63822020-06-019634510.3390/antibiotics9060345Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream InfectionsFernando Cobo0Jaime Borrego1Esther Gómez2Isabel Casanovas3Elizabeth Calatrava4Carla Foronda5José María Navarro-Marí6Department of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainDepartment of Microbiology and Instituto de Investigación Biosanitaria ibs. GRANADA, University Hospital Virgen de las Nieves, 18014 Granada, SpainThe objectives of this study were to report on the antimicrobial susceptibility of 141 clinically significant anaerobic bacteria isolated from bloodstream infections between January 2016 and April 2020 in a tertiary-care hospital in Granada (Spain) and to describe the main clinical features of the patients. Species identification was performed by MALDI-TOF MS (Bruker Daltonics, Billerica, MA, USA). Antimicrobial susceptibility tests were performed against penicillin, amoxicillin-clavulanic acid, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin-tazobactam using the gradient diffusion technique and EUCAST breakpoints, except for moxifloxacin (CLSI breakpoints). The most frequent anaerobes were <i>Bacteroides</i> (43.9%, <i>n</i> = 62), <i>Clostridium</i> (24.1%, <i>n</i> = 34) and Gram-positive anaerobic cocci (GPACs) (15.6%, <i>n</i> = 22). Almost all tested anaerobes were susceptible to imipenem and amoxicillin-clavulanic acid, except for <i>Bacteroides</i>. High overall resistance rates to clindamycin were observed, especially for Gram-positive anaerobic cocci (GPACs) (54.5%) and for <i>Bacteroides</i> spp. (45.1%). Overall, low resistance rates to almost all antibiotics were observed for <i>Clostridium</i>. High resistance rates to penicillin were also observed for Gram-positive anaerobic bacilli (GPABs) (44.4%), as well as to metronidazole (22.2%), although only nine isolates were included. Antimicrobial susceptibility testing for anaerobes should always be performed in severe infections, such as those localized in the bloodstream. The information obtained contributes to selecting empirical treatments according with local data on resistance.https://www.mdpi.com/2079-6382/9/6/345bloodstream infectionsantimicrobial susceptibility testingMALDI-TOF MSanaerobes |
| spellingShingle | Fernando Cobo Jaime Borrego Esther Gómez Isabel Casanovas Elizabeth Calatrava Carla Foronda José María Navarro-Marí Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections Antibiotics bloodstream infections antimicrobial susceptibility testing MALDI-TOF MS anaerobes |
| title | Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections |
| title_full | Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections |
| title_fullStr | Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections |
| title_full_unstemmed | Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections |
| title_short | Clinical Findings and Antimicrobial Susceptibility of Anaerobic Bacteria Isolated in Bloodstream Infections |
| title_sort | clinical findings and antimicrobial susceptibility of anaerobic bacteria isolated in bloodstream infections |
| topic | bloodstream infections antimicrobial susceptibility testing MALDI-TOF MS anaerobes |
| url | https://www.mdpi.com/2079-6382/9/6/345 |
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