Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i>
This study aimed to investigate the potential adverse effects of the practical application of copper sulfate on yellow catfish (<i>Pelteobagrus fulvidraco</i>) and to provide insights into the gill toxicity induced by copper sulphate. Yellow catfish were exposed to a conventional anthelm...
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MDPI AG
2023-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/6/1288 |
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| author | Shun Zhou Qiuhong Yang Yi Song Bo Cheng Xiaohui Ai |
| author_facet | Shun Zhou Qiuhong Yang Yi Song Bo Cheng Xiaohui Ai |
| author_sort | Shun Zhou |
| collection | DOAJ |
| description | This study aimed to investigate the potential adverse effects of the practical application of copper sulfate on yellow catfish (<i>Pelteobagrus fulvidraco</i>) and to provide insights into the gill toxicity induced by copper sulphate. Yellow catfish were exposed to a conventional anthelmintic concentration of copper sulphate (0.7 mg/L) for seven days. Oxidative stress biomarkers, transcriptome, and external microbiota of gills were examined using enzymatic assays, RNA-sequencing, and 16S rDNA analysis, respectively. Copper sulphate exposure led to oxidative stress and immunosuppression in the gills, with increased levels of oxidative stress biomarkers and altered expression of immune-related differentially expressed genes (DEGs), such as IL-1β, IL4Rα, and CCL24. Key pathways involved in the response included cytokine–cytokine receptor interaction, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway. The 16S rDNA analysis revealed copper sulphate altered the diversity and composition of gill microbiota, as evidenced by a significant decrease in the abundance of Bacteroidotas and Bdellovibrionota and a significant increase in the abundance of Proteobacteria. Notably, a substantial 8.5-fold increase in the abundance of <i>Plesiomonas</i> was also observed at the genus level. Our findings demonstrated that copper sulphate induced oxidative stress, immunosuppression, and gill microflora dysbiosis in yellow catfish. These findings highlight the need for sustainable management practices and alternative therapeutic strategies in the aquaculture industry to mitigate the adverse effects of copper sulphate on fish and other aquatic organisms. |
| first_indexed | 2024-03-11T02:50:15Z |
| format | Article |
| id | doaj.art-f6ab806e4509464584f89c247fdc1f2c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T02:50:15Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f6ab806e4509464584f89c247fdc1f2c2023-11-18T09:04:06ZengMDPI AGAntioxidants2076-39212023-06-01126128810.3390/antiox12061288Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i>Shun Zhou0Qiuhong Yang1Yi Song2Bo Cheng3Xiaohui Ai4Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaChinese Academy of Fishery Sciences, No.150, Qingta West Road, Fengtai District, Beijing 100141, ChinaChinese Academy of Fishery Sciences, No.150, Qingta West Road, Fengtai District, Beijing 100141, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaThis study aimed to investigate the potential adverse effects of the practical application of copper sulfate on yellow catfish (<i>Pelteobagrus fulvidraco</i>) and to provide insights into the gill toxicity induced by copper sulphate. Yellow catfish were exposed to a conventional anthelmintic concentration of copper sulphate (0.7 mg/L) for seven days. Oxidative stress biomarkers, transcriptome, and external microbiota of gills were examined using enzymatic assays, RNA-sequencing, and 16S rDNA analysis, respectively. Copper sulphate exposure led to oxidative stress and immunosuppression in the gills, with increased levels of oxidative stress biomarkers and altered expression of immune-related differentially expressed genes (DEGs), such as IL-1β, IL4Rα, and CCL24. Key pathways involved in the response included cytokine–cytokine receptor interaction, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway. The 16S rDNA analysis revealed copper sulphate altered the diversity and composition of gill microbiota, as evidenced by a significant decrease in the abundance of Bacteroidotas and Bdellovibrionota and a significant increase in the abundance of Proteobacteria. Notably, a substantial 8.5-fold increase in the abundance of <i>Plesiomonas</i> was also observed at the genus level. Our findings demonstrated that copper sulphate induced oxidative stress, immunosuppression, and gill microflora dysbiosis in yellow catfish. These findings highlight the need for sustainable management practices and alternative therapeutic strategies in the aquaculture industry to mitigate the adverse effects of copper sulphate on fish and other aquatic organisms.https://www.mdpi.com/2076-3921/12/6/1288copper sulphateyellow catfishoxidative stresstranscriptomeexternal microbiota |
| spellingShingle | Shun Zhou Qiuhong Yang Yi Song Bo Cheng Xiaohui Ai Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> Antioxidants copper sulphate yellow catfish oxidative stress transcriptome external microbiota |
| title | Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> |
| title_full | Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> |
| title_fullStr | Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> |
| title_full_unstemmed | Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> |
| title_short | Effect of Copper Sulphate Exposure on the Oxidative Stress, Gill Transcriptome and External Microbiota of Yellow Catfish, <i>Pelteobagrus fulvidraco</i> |
| title_sort | effect of copper sulphate exposure on the oxidative stress gill transcriptome and external microbiota of yellow catfish i pelteobagrus fulvidraco i |
| topic | copper sulphate yellow catfish oxidative stress transcriptome external microbiota |
| url | https://www.mdpi.com/2076-3921/12/6/1288 |
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