Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.)
Nitrogen (N), a macronutrient essential for plant growth and development, is needed for biosynthesis of protein and starch, which affect grain yield and quality. Application of high-N fertilizer increases plant growth, grain yield, and flour quality. In this study, we performed the first comparative...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-02-01
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| Series: | Crop Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514119300820 |
| _version_ | 1818849139666976768 |
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| author | Shoumin Zhen Xiong Deng Xuexin Xu Nannan Liu Dong Zhu Zhimin Wang Yueming Yan |
| author_facet | Shoumin Zhen Xiong Deng Xuexin Xu Nannan Liu Dong Zhu Zhimin Wang Yueming Yan |
| author_sort | Shoumin Zhen |
| collection | DOAJ |
| description | Nitrogen (N), a macronutrient essential for plant growth and development, is needed for biosynthesis of protein and starch, which affect grain yield and quality. Application of high-N fertilizer increases plant growth, grain yield, and flour quality. In this study, we performed the first comparative analysis of gliadin and glutenin subproteomes during kernel development in the elite Chinese wheat cultivar Zhongmai 175 under high-N conditions by reversed-phase ultra-performance liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE). Application of high-N fertilizer led to significant increases in gluten macropolymer content, total gliadin and glutenin content, and the accumulation of individual storage protein components. Of 126 differentially accumulated proteins (DAPs) induced by high-N conditions, 24 gliadins, 12 high-molecular-weight glutenins, and 27 low-molecular-weight glutenins were significantly upregulated. DAPs during five kernel developmental stages displayed multiple patterns of accumulation. In particular, gliadins and glutenins showed respectively five and six accumulation patterns. The accumulation of storage proteins under high-N conditions may lead to improved dough properties and bread quality. Keywords: Bread wheat, High nitrogen, Gliadins, Glutenins, RP-UPLC, 2D-DIGE |
| first_indexed | 2024-12-19T06:28:29Z |
| format | Article |
| id | doaj.art-f6ae960f2e0345ceba2409f0ca5f745f |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-19T06:28:29Z |
| publishDate | 2020-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-f6ae960f2e0345ceba2409f0ca5f745f2022-12-21T20:32:28ZengKeAi Communications Co., Ltd.Crop Journal2214-51412020-02-01813852Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.)Shoumin Zhen0Xiong Deng1Xuexin Xu2Nannan Liu3Dong Zhu4Zhimin Wang5Yueming Yan6College of Life Science, Capital Normal University, Beijing, China; Institute of Scientific and Technical Information of China, Beijing 100038, ChinaCollege of Life Science, Capital Normal University, Beijing, ChinaCollege of Agronomy and Biotechnology, China Agricultural University, 100081 Beijing, ChinaCollege of Life Science, Capital Normal University, Beijing, ChinaCollege of Life Science, Capital Normal University, Beijing, ChinaCollege of Agronomy and Biotechnology, China Agricultural University, 100081 Beijing, China; Corresponding author.College of Life Science, Capital Normal University, Beijing, China; Hubei Collaborative Innovation Center for Grain Industry (HCICGI), Yangtze University, 434025 Jingzhou, China; Correspondence to: Y. Yan, College of Life Science, Capital Normal University, Beijing, China.Nitrogen (N), a macronutrient essential for plant growth and development, is needed for biosynthesis of protein and starch, which affect grain yield and quality. Application of high-N fertilizer increases plant growth, grain yield, and flour quality. In this study, we performed the first comparative analysis of gliadin and glutenin subproteomes during kernel development in the elite Chinese wheat cultivar Zhongmai 175 under high-N conditions by reversed-phase ultra-performance liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE). Application of high-N fertilizer led to significant increases in gluten macropolymer content, total gliadin and glutenin content, and the accumulation of individual storage protein components. Of 126 differentially accumulated proteins (DAPs) induced by high-N conditions, 24 gliadins, 12 high-molecular-weight glutenins, and 27 low-molecular-weight glutenins were significantly upregulated. DAPs during five kernel developmental stages displayed multiple patterns of accumulation. In particular, gliadins and glutenins showed respectively five and six accumulation patterns. The accumulation of storage proteins under high-N conditions may lead to improved dough properties and bread quality. Keywords: Bread wheat, High nitrogen, Gliadins, Glutenins, RP-UPLC, 2D-DIGEhttp://www.sciencedirect.com/science/article/pii/S2214514119300820 |
| spellingShingle | Shoumin Zhen Xiong Deng Xuexin Xu Nannan Liu Dong Zhu Zhimin Wang Yueming Yan Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) Crop Journal |
| title | Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) |
| title_full | Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) |
| title_fullStr | Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) |
| title_short | Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.) |
| title_sort | effect of high nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat triticum aestivum l |
| url | http://www.sciencedirect.com/science/article/pii/S2214514119300820 |
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