Update on functional and genetic laboratory assays for the detection of platelet microvesicles
Functional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-04-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2016.1265925 |
| _version_ | 1827816962556690432 |
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| author | Maxime Laroche Claire Dunois Anne Marie Vissac Jean Amiral |
| author_facet | Maxime Laroche Claire Dunois Anne Marie Vissac Jean Amiral |
| author_sort | Maxime Laroche |
| collection | DOAJ |
| description | Functional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a thrombin generation assay (TGA) as “end points”; b) capture-based assays, in which PMVs bind to an immobilized ligand, such as Annexin V in the presence of calcium, or monoclonal antibodies (MoAbs) specific for membrane proteins. Genetic assays aim to detect micro-RNA (miRNA) present in PMVs: miRNA must be extracted from plasma, and the expression pattern can be determined by various methods such as quantitative real-time PCR, microarray or sequencing. All these technical approaches introduce new exploration tools for measuring or quantitating PMVs or their associated activities, as biomarkers for disease evolution, their diagnosis or prognosis, and for monitoring of some antithrombotic or anti-inflammatory therapies. They offer invaluable analytical tools for research, drug discovery and epidemiological studies and have a strong potential as diagnostic tests. |
| first_indexed | 2024-03-12T00:27:45Z |
| format | Article |
| id | doaj.art-f6b0cbf9e6714b89a58d8fd39b64c65d |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:45Z |
| publishDate | 2017-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-f6b0cbf9e6714b89a58d8fd39b64c65d2023-09-15T10:31:57ZengTaylor & Francis GroupPlatelets0953-71041369-16352017-04-0128323524110.1080/09537104.2016.12659251265925Update on functional and genetic laboratory assays for the detection of platelet microvesiclesMaxime Laroche0Claire Dunois1Anne Marie Vissac2Jean Amiral3HYPHEN BioMedHYPHEN BioMedHYPHEN BioMedScientific Consultant for HYPHEN BioMedFunctional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a thrombin generation assay (TGA) as “end points”; b) capture-based assays, in which PMVs bind to an immobilized ligand, such as Annexin V in the presence of calcium, or monoclonal antibodies (MoAbs) specific for membrane proteins. Genetic assays aim to detect micro-RNA (miRNA) present in PMVs: miRNA must be extracted from plasma, and the expression pattern can be determined by various methods such as quantitative real-time PCR, microarray or sequencing. All these technical approaches introduce new exploration tools for measuring or quantitating PMVs or their associated activities, as biomarkers for disease evolution, their diagnosis or prognosis, and for monitoring of some antithrombotic or anti-inflammatory therapies. They offer invaluable analytical tools for research, drug discovery and epidemiological studies and have a strong potential as diagnostic tests.http://dx.doi.org/10.1080/09537104.2016.1265925functional assaygenetic assaysmirnaplatelet microvesicles |
| spellingShingle | Maxime Laroche Claire Dunois Anne Marie Vissac Jean Amiral Update on functional and genetic laboratory assays for the detection of platelet microvesicles Platelets functional assay genetic assays mirna platelet microvesicles |
| title | Update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| title_full | Update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| title_fullStr | Update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| title_full_unstemmed | Update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| title_short | Update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| title_sort | update on functional and genetic laboratory assays for the detection of platelet microvesicles |
| topic | functional assay genetic assays mirna platelet microvesicles |
| url | http://dx.doi.org/10.1080/09537104.2016.1265925 |
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