Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii
Mansour Amin,1,2 Tahereh Navidifar,2 Farkhondeh Saleh Shooshtari,2 Hamed Goodarzi21Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Department of Microbiology, School of Medicine, Ahvaz Jundishapur University...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-05-01
|
| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/association-of-the-genes-encoding-metallo-beta-lactamase-with-the-pres-peer-reviewed-article-IDR |
| _version_ | 1828476968027291648 |
|---|---|
| author | Amin M Navidifar T Saleh Shooshtari F Goodarzi H |
| author_facet | Amin M Navidifar T Saleh Shooshtari F Goodarzi H |
| author_sort | Amin M |
| collection | DOAJ |
| description | Mansour Amin,1,2 Tahereh Navidifar,2 Farkhondeh Saleh Shooshtari,2 Hamed Goodarzi21Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Metallo-β-Lactamases (MBL) are usually encoded on the gene cassettes harboring integrons and disseminated easily among Acinetobacter baumannii isolates. This study was aimed to investigate the association of the genes encoding MBL with the presence of class 1 and 2 integrons among multidrug-resistant (MDR) A.baumannii isolates.Methodology: A total of 85 non-duplicated A.baumannii isolates were collected and evaluated for the amplification of blaOXA-51. The presence of genes encoding MBLs, including blaIMP, blaVIM, blaSIM, blaSPM, blaGIM, blaDIM and blaNDM, as well as intI 1 and intI 2 was evaluated by PCR. Also, the production of MBLs was screened phenotypically by the combination of EDTA and meropenem.Results: In this study, 77 out of 85 isolates were MDR. Also, 34 isolates had only intI 1, 10 had only intI 2 and 15 had both intI 1 and intI 2. The phenotypic detection of MBLs was found in 30 isolates, among which blaVIM was as the most common the gene encoding MBL followed by blaIMP, blaSPM and blaSIM. The gene cassettes analysis revealed that class 1 integron is often responsible for transferring the genes harboring MBLs.Conclusion: The production of MBLs among A. baumannii strains is one of the main mechanisms of resistance to carbapenems. Therefore, the development of inexpensive screening methods for the phenotypic detection of MBLs in clinical laboratories settings is essential. Also, our data revealed that the class 1 integron is often responsible for the dissemination of the MBL genes among A. baumannii isolates.Keywords: acinetobacter baumannii, blaVIM, blaIMP, integron, Metallo-Beta-Lactamase |
| first_indexed | 2024-12-11T06:36:25Z |
| format | Article |
| id | doaj.art-f6b9b705caad4d8cb87c9ee561392081 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-11T06:36:25Z |
| publishDate | 2019-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-f6b9b705caad4d8cb87c9ee5613920812022-12-22T01:17:22ZengDove Medical PressInfection and Drug Resistance1178-69732019-05-01Volume 121171118045761Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumanniiAmin MNavidifar TSaleh Shooshtari FGoodarzi HMansour Amin,1,2 Tahereh Navidifar,2 Farkhondeh Saleh Shooshtari,2 Hamed Goodarzi21Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Metallo-β-Lactamases (MBL) are usually encoded on the gene cassettes harboring integrons and disseminated easily among Acinetobacter baumannii isolates. This study was aimed to investigate the association of the genes encoding MBL with the presence of class 1 and 2 integrons among multidrug-resistant (MDR) A.baumannii isolates.Methodology: A total of 85 non-duplicated A.baumannii isolates were collected and evaluated for the amplification of blaOXA-51. The presence of genes encoding MBLs, including blaIMP, blaVIM, blaSIM, blaSPM, blaGIM, blaDIM and blaNDM, as well as intI 1 and intI 2 was evaluated by PCR. Also, the production of MBLs was screened phenotypically by the combination of EDTA and meropenem.Results: In this study, 77 out of 85 isolates were MDR. Also, 34 isolates had only intI 1, 10 had only intI 2 and 15 had both intI 1 and intI 2. The phenotypic detection of MBLs was found in 30 isolates, among which blaVIM was as the most common the gene encoding MBL followed by blaIMP, blaSPM and blaSIM. The gene cassettes analysis revealed that class 1 integron is often responsible for transferring the genes harboring MBLs.Conclusion: The production of MBLs among A. baumannii strains is one of the main mechanisms of resistance to carbapenems. Therefore, the development of inexpensive screening methods for the phenotypic detection of MBLs in clinical laboratories settings is essential. Also, our data revealed that the class 1 integron is often responsible for the dissemination of the MBL genes among A. baumannii isolates.Keywords: acinetobacter baumannii, blaVIM, blaIMP, integron, Metallo-Beta-Lactamasehttps://www.dovepress.com/association-of-the-genes-encoding-metallo-beta-lactamase-with-the-pres-peer-reviewed-article-IDRAcinetobacter baumanniiblaVIMblaIMPintegronMetallo-Beta-Lactamase |
| spellingShingle | Amin M Navidifar T Saleh Shooshtari F Goodarzi H Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii Infection and Drug Resistance Acinetobacter baumannii blaVIM blaIMP integron Metallo-Beta-Lactamase |
| title | Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii |
| title_full | Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii |
| title_fullStr | Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii |
| title_full_unstemmed | Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii |
| title_short | Association of the genes encoding Metallo-β-Lactamase with the presence of integrons among multidrug-resistant clinical isolates of Acinetobacter baumannii |
| title_sort | association of the genes encoding metallo beta lactamase with the presence of integrons among multidrug resistant clinical isolates of acinetobacter baumannii |
| topic | Acinetobacter baumannii blaVIM blaIMP integron Metallo-Beta-Lactamase |
| url | https://www.dovepress.com/association-of-the-genes-encoding-metallo-beta-lactamase-with-the-pres-peer-reviewed-article-IDR |
| work_keys_str_mv | AT aminm associationofthegenesencodingmetallobetalactamasewiththepresenceofintegronsamongmultidrugresistantclinicalisolatesofacinetobacterbaumannii AT navidifart associationofthegenesencodingmetallobetalactamasewiththepresenceofintegronsamongmultidrugresistantclinicalisolatesofacinetobacterbaumannii AT salehshooshtarif associationofthegenesencodingmetallobetalactamasewiththepresenceofintegronsamongmultidrugresistantclinicalisolatesofacinetobacterbaumannii AT goodarzih associationofthegenesencodingmetallobetalactamasewiththepresenceofintegronsamongmultidrugresistantclinicalisolatesofacinetobacterbaumannii |