Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection
Bladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for t...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/5/1313 |
| _version_ | 1797542088607268864 |
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| author | Pushap Raj Seon-yeong Lee Tae Yoon Lee |
| author_facet | Pushap Raj Seon-yeong Lee Tae Yoon Lee |
| author_sort | Pushap Raj |
| collection | DOAJ |
| description | Bladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for the early recognition of HAase. Herein, we report a simple, sensitive, and ratiometric fluorescence assay for HAase detection under physiological conditions. The fluorescence assay was constructed by the adsorption of cationic carbon dots and positively charged naphthalimide on negatively charged hyaluronic acid and the development of a Förster resonance energy transfer (FRET) mechanism from carbon dots to a naphthalimide fluorophores. The hyaluronidase enzyme cleaves the hyaluronic acid in this assay, and breaking down the FRET mechanism induces ratiometric changes. A detection limit of 0.09 U/mL was achieved, which is less than the HAase level found in normal human body fluids. Moreover, this assay may be used for diagnosing HAase-related diseases. |
| first_indexed | 2024-03-10T13:24:43Z |
| format | Article |
| id | doaj.art-f6bbf19b371d41d98968e6200bc8bfaf |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T13:24:43Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-f6bbf19b371d41d98968e6200bc8bfaf2023-11-21T09:46:38ZengMDPI AGMaterials1996-19442021-03-01145131310.3390/ma14051313Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase DetectionPushap Raj0Seon-yeong Lee1Tae Yoon Lee2Department of Convergence System Engineering and Department of Biomedical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Technology Education, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Convergence System Engineering and Department of Biomedical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaBladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for the early recognition of HAase. Herein, we report a simple, sensitive, and ratiometric fluorescence assay for HAase detection under physiological conditions. The fluorescence assay was constructed by the adsorption of cationic carbon dots and positively charged naphthalimide on negatively charged hyaluronic acid and the development of a Förster resonance energy transfer (FRET) mechanism from carbon dots to a naphthalimide fluorophores. The hyaluronidase enzyme cleaves the hyaluronic acid in this assay, and breaking down the FRET mechanism induces ratiometric changes. A detection limit of 0.09 U/mL was achieved, which is less than the HAase level found in normal human body fluids. Moreover, this assay may be used for diagnosing HAase-related diseases.https://www.mdpi.com/1996-1944/14/5/1313fluorescencehyaluronic acidbiosensorhyaluronidasenaphthalimide |
| spellingShingle | Pushap Raj Seon-yeong Lee Tae Yoon Lee Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection Materials fluorescence hyaluronic acid biosensor hyaluronidase naphthalimide |
| title | Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection |
| title_full | Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection |
| title_fullStr | Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection |
| title_full_unstemmed | Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection |
| title_short | Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection |
| title_sort | carbon dot naphthalimide based ratiometric fluorescence biosensor for hyaluronidase detection |
| topic | fluorescence hyaluronic acid biosensor hyaluronidase naphthalimide |
| url | https://www.mdpi.com/1996-1944/14/5/1313 |
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