Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp
Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times. Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine. Artemisia is a genus...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| author | Jawaher J. Albaqami Tancia P. Benny Hamida Hamdi Ammar B. Altemimi Aswathi Moothakoottil Kuttithodi Joice Tom Job Anju Sasidharan Arunaksharan Narayanankutty |
| author_facet | Jawaher J. Albaqami Tancia P. Benny Hamida Hamdi Ammar B. Altemimi Aswathi Moothakoottil Kuttithodi Joice Tom Job Anju Sasidharan Arunaksharan Narayanankutty |
| author_sort | Jawaher J. Albaqami |
| collection | DOAJ |
| description | Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times. Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine. Artemisia is a genus of the <i>Asteraceae</i> family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses. <i>Artemisia nilagirica</i> is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus. In the current study, the phytochemical composition and the antioxidant, enzyme-inhibitory, anti-inflammatory, and anticancer activities were examined. The results indicated that the ethanol extract of <i>A. nilagirica</i> indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H<sub>2</sub>O<sub>2</sub> scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL). The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (<i>p</i> < 0.05). <i>A. nilagirica</i> was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL). It also induced significant inhibition of proliferation in breast (MCF7 IC<sub>50</sub> = 41.79 ± 1.07, MDAMB231 IC<sub>50</sub> = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells. The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin. |
| first_indexed | 2024-03-09T19:41:56Z |
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| language | English |
| last_indexed | 2024-03-09T19:41:56Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-f6bc6a6bfa06490fa17d0e65e1f7bd922023-11-24T01:37:48ZengMDPI AGMolecules1420-30492022-10-012720711910.3390/molecules27207119Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) PampJawaher J. Albaqami0Tancia P. Benny1Hamida Hamdi2Ammar B. Altemimi3Aswathi Moothakoottil Kuttithodi4Joice Tom Job5Anju Sasidharan6Arunaksharan Narayanankutty7Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDivision of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph’s College (Autonomous), Devagiri, Calicut 673 008, Kerala, IndiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Food Science, College of Agriculture, University of Basrah, Basrah 61004, IraqDivision of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph’s College (Autonomous), Devagiri, Calicut 673 008, Kerala, IndiaDivision of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph’s College (Autonomous), Devagiri, Calicut 673 008, Kerala, IndiaDivision of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph’s College (Autonomous), Devagiri, Calicut 673 008, Kerala, IndiaDivision of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph’s College (Autonomous), Devagiri, Calicut 673 008, Kerala, IndiaPlants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times. Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine. Artemisia is a genus of the <i>Asteraceae</i> family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses. <i>Artemisia nilagirica</i> is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus. In the current study, the phytochemical composition and the antioxidant, enzyme-inhibitory, anti-inflammatory, and anticancer activities were examined. The results indicated that the ethanol extract of <i>A. nilagirica</i> indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H<sub>2</sub>O<sub>2</sub> scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL). The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (<i>p</i> < 0.05). <i>A. nilagirica</i> was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL). It also induced significant inhibition of proliferation in breast (MCF7 IC<sub>50</sub> = 41.79 ± 1.07, MDAMB231 IC<sub>50</sub> = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells. The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.https://www.mdpi.com/1420-3049/27/20/7119phytochemistry<i>Artemisia nilagirica</i><i>Asteraceae</i>antioxidantanti-inflammatory activityanticancer activity |
| spellingShingle | Jawaher J. Albaqami Tancia P. Benny Hamida Hamdi Ammar B. Altemimi Aswathi Moothakoottil Kuttithodi Joice Tom Job Anju Sasidharan Arunaksharan Narayanankutty Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp Molecules phytochemistry <i>Artemisia nilagirica</i> <i>Asteraceae</i> antioxidant anti-inflammatory activity anticancer activity |
| title | Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp |
| title_full | Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp |
| title_fullStr | Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp |
| title_full_unstemmed | Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp |
| title_short | Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of <i>Artemisia nilagirica</i> (C.B. Clarke) Pamp |
| title_sort | phytochemical composition and in vitro antioxidant anti inflammatory anticancer and enzyme inhibitory activities of i artemisia nilagirica i c b clarke pamp |
| topic | phytochemistry <i>Artemisia nilagirica</i> <i>Asteraceae</i> antioxidant anti-inflammatory activity anticancer activity |
| url | https://www.mdpi.com/1420-3049/27/20/7119 |
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