Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response
Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we sh...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2016-01-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150541 |
| _version_ | 1818342064441524224 |
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| author | William D. K. Reid Andrew J. Close Suzanne Humphrey Gemma Chaloner Lizeth Lacharme-Lora Lisa Rothwell Pete Kaiser Nicola J. Williams Tom J. Humphrey Paul Wigley Stephen P. Rushton |
| author_facet | William D. K. Reid Andrew J. Close Suzanne Humphrey Gemma Chaloner Lizeth Lacharme-Lora Lisa Rothwell Pete Kaiser Nicola J. Williams Tom J. Humphrey Paul Wigley Stephen P. Rushton |
| author_sort | William D. K. Reid |
| collection | DOAJ |
| description | Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, , IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed-dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonization of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds. |
| first_indexed | 2024-12-13T16:08:45Z |
| format | Article |
| id | doaj.art-f6bccd199ef2444ca1600d569bb5831e |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-13T16:08:45Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-f6bccd199ef2444ca1600d569bb5831e2022-12-21T23:39:00ZengThe Royal SocietyRoyal Society Open Science2054-57032016-01-013310.1098/rsos.150541150541Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 responseWilliam D. K. ReidAndrew J. CloseSuzanne HumphreyGemma ChalonerLizeth Lacharme-LoraLisa RothwellPete KaiserNicola J. WilliamsTom J. HumphreyPaul WigleyStephen P. RushtonDevelopment of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, , IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed-dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonization of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150541gastrointestinal tractth1 and th17 responsestructural equation modelbacterial pathogengamma-delta t lymphocytesinflammation |
| spellingShingle | William D. K. Reid Andrew J. Close Suzanne Humphrey Gemma Chaloner Lizeth Lacharme-Lora Lisa Rothwell Pete Kaiser Nicola J. Williams Tom J. Humphrey Paul Wigley Stephen P. Rushton Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response Royal Society Open Science gastrointestinal tract th1 and th17 response structural equation model bacterial pathogen gamma-delta t lymphocytes inflammation |
| title | Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response |
| title_full | Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response |
| title_fullStr | Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response |
| title_full_unstemmed | Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response |
| title_short | Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response |
| title_sort | cytokine responses in birds challenged with the human food borne pathogen campylobacter jejuni implies a th17 response |
| topic | gastrointestinal tract th1 and th17 response structural equation model bacterial pathogen gamma-delta t lymphocytes inflammation |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150541 |
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