Development of a method for determination of fatty acid using FT-IR spectroscopy
In the present paper a new methodology has been developed for determination of fatty acids in biological systems using FT-IR spectroscopy. For this method is not necessary chromophore reagent or pre sample preparation. Palmitic acid was chosen as standard, because it is found in several biological s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Londrina
2011-05-01
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| Series: | Semina: Ciências Exatas e Tecnológicas |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semexatas/article/view/6481 |
| Summary: | In the present paper a new methodology has been developed for determination of fatty acids in biological systems using FT-IR spectroscopy. For this method is not necessary chromophore reagent or pre sample preparation. Palmitic acid was chosen as standard, because it is found in several biological systems. The FT-IR spectrum of palmitic acid showed two absorption bands in the region of 2852 and 2920 cm-1 attributed to CH stretching. The results for these bands showed that the Beer-Lambert Law was followed in wide range of concentration of palmitic acid (14 to 257 mmol L-1). Potassium ferricyanide (K3[Fe(CN)6]) was used as internal standard. Several interferents were tested and only cholesterol, ferric chloride (higher concentration), mixture of amino acids for the band at 2919 cm-1 (higher concentration) and triglyceride could be interferent if they appear in high concentration. Thus, this new methodology has advantage to be not expensive and simple. |
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| ISSN: | 1676-5451 1679-0375 |