Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis
Background and Objective: Helicobacter pylori are one of the most common gastrointestinal bacterium infections. This study was done to compare the urease test with Giemsa staining to detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis. Methods: This descriptive –...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Golestan University of Medical Sciences
2017-03-01
|
Series: | مجله دانشگاه علوم پزشکی گرگان |
Subjects: | |
Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-964&slc_lang=en&sid=1 |
_version_ | 1818580236185370624 |
---|---|
author | R Davaloo H Kaboosi Kh Heidari R Azarhoush E Naeimi-Tabiei V Kazeminejad |
author_facet | R Davaloo H Kaboosi Kh Heidari R Azarhoush E Naeimi-Tabiei V Kazeminejad |
author_sort | R Davaloo |
collection | DOAJ |
description | Background and Objective: Helicobacter pylori are one of the most common gastrointestinal bacterium infections. This study was done to compare the urease test with Giemsa staining to detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis.
Methods: This descriptive – analytic study was conducted on 601 patients' diagnosed with gastric ulcer and gastritis, whom under went endoscopy in 5th Azar hospital, Gorgan, Iran during 2008-12. Rapid urease test was immediately taken during endoscopy. Pathological examination, Giemsa staining on paraffin blocks and haematoxylin and eosin stain were done to assess either presence or absence of Helicobacter pylori.
Results: Based on biopsy results, Gastritis and gastric ulcer were diagnosed in 80.69% and 19.3%, respectively. In gastritis and gastric ulcer patients, there was a significant differences between urease test (91.5% and 90%, respectively) in comparison with Giemsa staining (91.5% and 90%, respectively) (P<0.05).
Conclusion: The study revealed that in primary diagnosis and screening of role of Helicobacter pylori in patients with gastric ulcer and gastritis, urease test is suitable than giemsa staining. |
first_indexed | 2024-12-16T07:14:23Z |
format | Article |
id | doaj.art-f6be8bbb65c44a51b1f1baabc534100e |
institution | Directory Open Access Journal |
issn | 1562-4765 2008-4080 |
language | fas |
last_indexed | 2024-12-16T07:14:23Z |
publishDate | 2017-03-01 |
publisher | Golestan University of Medical Sciences |
record_format | Article |
series | مجله دانشگاه علوم پزشکی گرگان |
spelling | doaj.art-f6be8bbb65c44a51b1f1baabc534100e2022-12-21T22:39:50ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802017-03-011916771Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritisR Davaloo0H Kaboosi1Kh Heidari2R Azarhoush3E Naeimi-Tabiei4V Kazeminejad5 General Physician, Golestan Univercity of Medical Sciences, Gorgan, Iran Assistant Professor, Department of Microbiology, Ayat Ollah Amoli Branch, Islamic Azad University, Amol, Iran Ph.D Candidate in Microbiology, Ayat Ollah Amoli Branch, Islamic Azad University, Amol, Iran Associate Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran Assistant Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran Assistant Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran Background and Objective: Helicobacter pylori are one of the most common gastrointestinal bacterium infections. This study was done to compare the urease test with Giemsa staining to detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis. Methods: This descriptive – analytic study was conducted on 601 patients' diagnosed with gastric ulcer and gastritis, whom under went endoscopy in 5th Azar hospital, Gorgan, Iran during 2008-12. Rapid urease test was immediately taken during endoscopy. Pathological examination, Giemsa staining on paraffin blocks and haematoxylin and eosin stain were done to assess either presence or absence of Helicobacter pylori. Results: Based on biopsy results, Gastritis and gastric ulcer were diagnosed in 80.69% and 19.3%, respectively. In gastritis and gastric ulcer patients, there was a significant differences between urease test (91.5% and 90%, respectively) in comparison with Giemsa staining (91.5% and 90%, respectively) (P<0.05). Conclusion: The study revealed that in primary diagnosis and screening of role of Helicobacter pylori in patients with gastric ulcer and gastritis, urease test is suitable than giemsa staining.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-964&slc_lang=en&sid=1Helicobacter pyloriGastritisGastric ulcerGiemsa stainingUrease test |
spellingShingle | R Davaloo H Kaboosi Kh Heidari R Azarhoush E Naeimi-Tabiei V Kazeminejad Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis مجله دانشگاه علوم پزشکی گرگان Helicobacter pylori Gastritis Gastric ulcer Giemsa staining Urease test |
title | Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis |
title_full | Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis |
title_fullStr | Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis |
title_full_unstemmed | Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis |
title_short | Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis |
title_sort | compraision of urease test with giemsa staining for detection of helicobacter pylori infection in patients with gastric ulcer and gastritis |
topic | Helicobacter pylori Gastritis Gastric ulcer Giemsa staining Urease test |
url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-964&slc_lang=en&sid=1 |
work_keys_str_mv | AT rdavaloo compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis AT hkaboosi compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis AT khheidari compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis AT razarhoush compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis AT enaeimitabiei compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis AT vkazeminejad compraisionofureasetestwithgiemsastainingfordetectionofhelicobacterpyloriinfectioninpatientswithgastriculcerandgastritis |