Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children
Objective: Interleukin-5 (IL-5) is a cytokine known to play major role in the regulation of eosinophil formation, maturation, recruitment, and survival. Hence, an increased production of IL-5 may be contributed to the pathogenesis of eosinophil-dependent inflammatory diseases such as asthma. Methods...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2019;volume=3;issue=3;spage=156;epage=161;aulast=Hameed |
| _version_ | 1818562003438927872 |
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| author | Raghdah Maytham Hameed Mohanad Mohsin Ahmed Haidar Abdul Amir Najim Abood |
| author_facet | Raghdah Maytham Hameed Mohanad Mohsin Ahmed Haidar Abdul Amir Najim Abood |
| author_sort | Raghdah Maytham Hameed |
| collection | DOAJ |
| description | Objective: Interleukin-5 (IL-5) is a cytokine known to play major role in the regulation of eosinophil formation, maturation, recruitment, and survival. Hence, an increased production of IL-5 may be contributed to the pathogenesis of eosinophil-dependent inflammatory diseases such as asthma. Methods: A total of 85 children, including 59 males and 26 females with asthma with ages between 1 year and 16 years, attended the Respiratory Clinic at Karbala pediatric hospital, with a nonasthmatic children group which have the same age and gender. Restriction fragment length polymerase chain reaction was performed to determine IL-5 C-703T genetic polymorphisms. Total immunoglobulin E (IgE) level was measured using the EUROIMMUN IgE ELISA kit and serum IL-5 levels using Elabscience ELISA kit. The absolute eosinophil count was measured by five differential automated hematology analyzers and confirmed by the examination of peripheral blood smear. Results: The TT genotype of IL-5 C-703T polymorphism was associated with asthmatic children (P: 0.033). In addition, TT genotype was associated with increase of serum IL-5 levels and high absolute eosinophil count (P = 0.008 and 0.021, respectively). Whereas, CT genotype of IL-5 C-703T was found to be associated with high total serum IgE (P < 0.001). Conclusions: Asthma in children is associated with IL-5 C-703T polymorphism. In addition, IL-5 C-703T polymorphism has impact on IL-5 levels and eosinophil count. TT genotype of IL-5 C703T consider a risk factor for mild asthma in Iraqi asthmatic children. |
| first_indexed | 2024-12-14T00:58:05Z |
| format | Article |
| id | doaj.art-f6c247bf4c474be5896a74601f72c02b |
| institution | Directory Open Access Journal |
| issn | 2588-9834 2588-9842 |
| language | English |
| last_indexed | 2024-12-14T00:58:05Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj.art-f6c247bf4c474be5896a74601f72c02b2022-12-21T23:23:25ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422019-01-013315616110.4103/bbrj.bbrj_90_19Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic childrenRaghdah Maytham HameedMohanad Mohsin AhmedHaidar Abdul Amir Najim AboodObjective: Interleukin-5 (IL-5) is a cytokine known to play major role in the regulation of eosinophil formation, maturation, recruitment, and survival. Hence, an increased production of IL-5 may be contributed to the pathogenesis of eosinophil-dependent inflammatory diseases such as asthma. Methods: A total of 85 children, including 59 males and 26 females with asthma with ages between 1 year and 16 years, attended the Respiratory Clinic at Karbala pediatric hospital, with a nonasthmatic children group which have the same age and gender. Restriction fragment length polymerase chain reaction was performed to determine IL-5 C-703T genetic polymorphisms. Total immunoglobulin E (IgE) level was measured using the EUROIMMUN IgE ELISA kit and serum IL-5 levels using Elabscience ELISA kit. The absolute eosinophil count was measured by five differential automated hematology analyzers and confirmed by the examination of peripheral blood smear. Results: The TT genotype of IL-5 C-703T polymorphism was associated with asthmatic children (P: 0.033). In addition, TT genotype was associated with increase of serum IL-5 levels and high absolute eosinophil count (P = 0.008 and 0.021, respectively). Whereas, CT genotype of IL-5 C-703T was found to be associated with high total serum IgE (P < 0.001). Conclusions: Asthma in children is associated with IL-5 C-703T polymorphism. In addition, IL-5 C-703T polymorphism has impact on IL-5 levels and eosinophil count. TT genotype of IL-5 C703T consider a risk factor for mild asthma in Iraqi asthmatic children.http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2019;volume=3;issue=3;spage=156;epage=161;aulast=HameedBronchial asthmaeosinophilinterleukin 5total IgE |
| spellingShingle | Raghdah Maytham Hameed Mohanad Mohsin Ahmed Haidar Abdul Amir Najim Abood Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children Biomedical and Biotechnology Research Journal Bronchial asthma eosinophil interleukin 5 total IgE |
| title | Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children |
| title_full | Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children |
| title_fullStr | Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children |
| title_full_unstemmed | Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children |
| title_short | Specific IL-5 snp is associated with high serum IL-5 levels and higher eosinophil counts among iraqi asthmatic children |
| title_sort | specific il 5 snp is associated with high serum il 5 levels and higher eosinophil counts among iraqi asthmatic children |
| topic | Bronchial asthma eosinophil interleukin 5 total IgE |
| url | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2019;volume=3;issue=3;spage=156;epage=161;aulast=Hameed |
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