CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum)
Heat stress, an important and damaging abiotic stress, regulates numerous WRKY transcription factors, but their roles in heat stress responses remain largely unexplored. Here, we show that pepper (Capsicum annuum) CaWRKY27 negatively regulates basal thermotolerance mediated by H2O2 signaling. CaWRKY...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01633/full |
| _version_ | 1818037579450155008 |
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| author | Fengfeng Dang Jinhui Lin Jinhui Lin Baoping Xue Yongping Chen Deyi Guan Deyi Guan Yanfeng Wang Shuilin He Shuilin He Shuilin He |
| author_facet | Fengfeng Dang Jinhui Lin Jinhui Lin Baoping Xue Yongping Chen Deyi Guan Deyi Guan Yanfeng Wang Shuilin He Shuilin He Shuilin He |
| author_sort | Fengfeng Dang |
| collection | DOAJ |
| description | Heat stress, an important and damaging abiotic stress, regulates numerous WRKY transcription factors, but their roles in heat stress responses remain largely unexplored. Here, we show that pepper (Capsicum annuum) CaWRKY27 negatively regulates basal thermotolerance mediated by H2O2 signaling. CaWRKY27 expression increased during heat stress and persisted during recovery. CaWRKY27 overexpression impaired basal thermotolerance in tobacco (Nicotiana tabacum) and Arabidopsis thaliana, CaWRKY27-overexpressing plants had a lower survival rate under heat stress, accompanied by decreased expression of multiple thermotolerance-associated genes. Accordingly, silencing of CaWRKY27 increased basal thermotolerance in pepper plants. Exogenously applied H2O2 induced CaWRKY27 expression, and CaWRKY27 overexpression repressed the scavenging of H2O2 in Arabidopsis, indicating a positive feedback loop between H2O2 accumulation and CaWRKY27 expression. Consistent with this, CaWRKY27 expression was repressed under heat stress in the presence H2O2 scavengers and CaWRKY27 silencing decreased H2O2 accumulation in pepper leaves. These changes may result from changes in levels of reactive oxygen species (ROS)-scavenging enzymes, since the heat stress-challenged CaWRKY27-silenced pepper plants had significantly higher expression of multiple genes encoding ROS-scavenging enzymes, such as CaCAT1, CaAPX1, CaAPX2, CaCSD2, and CaSOD1. Therefore, CaWRKY27 acts as a downstream negative regulator of H2O2-mediated heat stress responses, preventing inappropriate responses during heat stress and recovery. |
| first_indexed | 2024-12-10T07:29:05Z |
| format | Article |
| id | doaj.art-f6c372161c984e2bb0338ac78cecff65 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T07:29:05Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f6c372161c984e2bb0338ac78cecff652022-12-22T01:57:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-11-01910.3389/fpls.2018.01633361624CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum)Fengfeng Dang0Jinhui Lin1Jinhui Lin2Baoping Xue3Yongping Chen4Deyi Guan5Deyi Guan6Yanfeng Wang7Shuilin He8Shuilin He9Shuilin He10National Education Minister, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Applied Genetics of Universities in Fujian Province, Fuzhou, ChinaCollege of Life Science, Yan’an University, Yan’an, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Applied Genetics of Universities in Fujian Province, Fuzhou, ChinaCollege of Life Science, Yan’an University, Yan’an, ChinaNational Education Minister, Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Applied Genetics of Universities in Fujian Province, Fuzhou, ChinaHeat stress, an important and damaging abiotic stress, regulates numerous WRKY transcription factors, but their roles in heat stress responses remain largely unexplored. Here, we show that pepper (Capsicum annuum) CaWRKY27 negatively regulates basal thermotolerance mediated by H2O2 signaling. CaWRKY27 expression increased during heat stress and persisted during recovery. CaWRKY27 overexpression impaired basal thermotolerance in tobacco (Nicotiana tabacum) and Arabidopsis thaliana, CaWRKY27-overexpressing plants had a lower survival rate under heat stress, accompanied by decreased expression of multiple thermotolerance-associated genes. Accordingly, silencing of CaWRKY27 increased basal thermotolerance in pepper plants. Exogenously applied H2O2 induced CaWRKY27 expression, and CaWRKY27 overexpression repressed the scavenging of H2O2 in Arabidopsis, indicating a positive feedback loop between H2O2 accumulation and CaWRKY27 expression. Consistent with this, CaWRKY27 expression was repressed under heat stress in the presence H2O2 scavengers and CaWRKY27 silencing decreased H2O2 accumulation in pepper leaves. These changes may result from changes in levels of reactive oxygen species (ROS)-scavenging enzymes, since the heat stress-challenged CaWRKY27-silenced pepper plants had significantly higher expression of multiple genes encoding ROS-scavenging enzymes, such as CaCAT1, CaAPX1, CaAPX2, CaCSD2, and CaSOD1. Therefore, CaWRKY27 acts as a downstream negative regulator of H2O2-mediated heat stress responses, preventing inappropriate responses during heat stress and recovery.https://www.frontiersin.org/article/10.3389/fpls.2018.01633/fullCapsicum annuumabiotic stressthermotoleranceCaWRKY27H2O2 |
| spellingShingle | Fengfeng Dang Jinhui Lin Jinhui Lin Baoping Xue Yongping Chen Deyi Guan Deyi Guan Yanfeng Wang Shuilin He Shuilin He Shuilin He CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) Frontiers in Plant Science Capsicum annuum abiotic stress thermotolerance CaWRKY27 H2O2 |
| title | CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) |
| title_full | CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) |
| title_fullStr | CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) |
| title_full_unstemmed | CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) |
| title_short | CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum) |
| title_sort | cawrky27 negatively regulates h2o2 mediated thermotolerance in pepper capsicum annuum |
| topic | Capsicum annuum abiotic stress thermotolerance CaWRKY27 H2O2 |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01633/full |
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