Summary: | Expression of rdar (red, dry, and rough) colony morphology-based biofilm formation in <i>Escherichia coli</i> is highly variable. To investigate the molecular mechanisms of semi-constitutive rdar morphotype formation, we compared their cyclic di-GMP turnover protein content and variability to the highly regulated, temperature-dependent morphotype of the historical and modern ST10 isolates <i>E. coli</i> MG1655 and Fec10, respectively. Subsequently, we assessed the effects of cyclic di-GMP turnover protein variants of the EAL phosphodiesterases YcgG and YjcC and the horizontally transferred diguanylate cyclase DgcX on biofilm formation and motility. The two YcgG variants with truncations of the N-terminal CSS signaling domain were oppositely effective in targeting downregulation of rdar biofilm formation compared to the full-length reference protein. Expression of the C-terminal truncated variants YjcC<sub>Fec67</sub> and YjcC<sub>Tob1</sub> showed highly diminished apparent phosphodiesterase activity compared to the reference YjcC<sub>MG1655</sub>. For YjcC<sub>Fec101</sub>, substitution of the C-terminus led to an apparently inactive enzyme. Overexpression of the diguanylate cyclase DgcX contributed to upregulation of cellulose biosynthesis but not to elevated expression of the major biofilm regulator <i>csgD</i> in the “classical” rdar-expressing commensal strain <i>E. coli</i> Fec10. Thus, the c-di-GMP regulating network is highly complex with protein variants displaying substantially different apparent enzymatic activities.
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