Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection
Porcine reproductive and respiratory syndrome virus (PRRSV) poses a global threat to pig health and results in significant economic losses. Impaired innate and adaptive immune responses are evident during PRRSV infection. Cyclic GMP-AMP synthase (cGAS), a classical pattern recognition receptor recog...
| Główni autorzy: | , , , , , |
|---|---|
| Format: | Artykuł |
| Język: | English |
| Wydane: |
MDPI AG
2023-07-01
|
| Seria: | Viruses |
| Hasła przedmiotowe: | |
| Dostęp online: | https://www.mdpi.com/1999-4915/15/7/1586 |
| _version_ | 1827731555336847360 |
|---|---|
| author | Ke Song Jia-Ying Yu Jiang Li Miao Li Lu-Yuan Peng Peng-Fei Yi |
| author_facet | Ke Song Jia-Ying Yu Jiang Li Miao Li Lu-Yuan Peng Peng-Fei Yi |
| author_sort | Ke Song |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) poses a global threat to pig health and results in significant economic losses. Impaired innate and adaptive immune responses are evident during PRRSV infection. Cyclic GMP-AMP synthase (cGAS), a classical pattern recognition receptor recognizing mainly intracytoplasmic DNA, induces type I IFN responses through the cGAS-STING signaling pathway. It has also been demonstrated that cGAS-STING is involved in PRRSV infection. This study utilized the qRT-PCR, ELISA, and WB methods to examine the effects of Astragaloside IV (AS-IV) on the regulation of innate immune function and cGAS-STING signaling pathway in porcine alveolar macrophages. The results showed that AS-IV attenuated the decreased innate immune function caused by PRRSV infection, restored the inhibited cGAS-STING signaling pathway, and increased the expression of interferon, ultimately exerting antiviral effects. Moreover, these results suggest that AS-IV may be a promising candidate for a new anti-PRRSV antiviral, and its mechanism of action may provide insights for developing novel antiviral agents. |
| first_indexed | 2024-03-11T00:33:48Z |
| format | Article |
| id | doaj.art-f6c68b0a63b34c69b2443b766d9a4379 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T00:33:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f6c68b0a63b34c69b2443b766d9a43792023-11-18T21:45:46ZengMDPI AGViruses1999-49152023-07-01157158610.3390/v15071586Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV InfectionKe Song0Jia-Ying Yu1Jiang Li2Miao Li3Lu-Yuan Peng4Peng-Fei Yi5College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) poses a global threat to pig health and results in significant economic losses. Impaired innate and adaptive immune responses are evident during PRRSV infection. Cyclic GMP-AMP synthase (cGAS), a classical pattern recognition receptor recognizing mainly intracytoplasmic DNA, induces type I IFN responses through the cGAS-STING signaling pathway. It has also been demonstrated that cGAS-STING is involved in PRRSV infection. This study utilized the qRT-PCR, ELISA, and WB methods to examine the effects of Astragaloside IV (AS-IV) on the regulation of innate immune function and cGAS-STING signaling pathway in porcine alveolar macrophages. The results showed that AS-IV attenuated the decreased innate immune function caused by PRRSV infection, restored the inhibited cGAS-STING signaling pathway, and increased the expression of interferon, ultimately exerting antiviral effects. Moreover, these results suggest that AS-IV may be a promising candidate for a new anti-PRRSV antiviral, and its mechanism of action may provide insights for developing novel antiviral agents.https://www.mdpi.com/1999-4915/15/7/1586AS-IVPRRSVcGAS-STINGimmunosuppressionantiviral activity |
| spellingShingle | Ke Song Jia-Ying Yu Jiang Li Miao Li Lu-Yuan Peng Peng-Fei Yi Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection Viruses AS-IV PRRSV cGAS-STING immunosuppression antiviral activity |
| title | Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection |
| title_full | Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection |
| title_fullStr | Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection |
| title_full_unstemmed | Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection |
| title_short | Astragaloside IV Regulates cGAS-STING Signaling Pathway to Alleviate Immunosuppression Caused by PRRSV Infection |
| title_sort | astragaloside iv regulates cgas sting signaling pathway to alleviate immunosuppression caused by prrsv infection |
| topic | AS-IV PRRSV cGAS-STING immunosuppression antiviral activity |
| url | https://www.mdpi.com/1999-4915/15/7/1586 |
| work_keys_str_mv | AT kesong astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection AT jiayingyu astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection AT jiangli astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection AT miaoli astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection AT luyuanpeng astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection AT pengfeiyi astragalosideivregulatescgasstingsignalingpathwaytoalleviateimmunosuppressioncausedbyprrsvinfection |