Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model
Several novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia in 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two novel genotypes...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | English |
| Publicado: |
Taylor & Francis Group
2022-12-01
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| Colección: | Emerging Microbes and Infections |
| Materias: | |
| Acceso en línea: | https://www.tandfonline.com/doi/10.1080/22221751.2022.2069515 |
| _version_ | 1828731166724718592 |
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| author | Bao Tuan Duong Duc Duong Than Ulaankhuu Ankhanbaatar Delgerzul Gombo-Ochir Gansukh Shura Amartuvshin Tsolmon Chris Ka Pun Mok Ganzorig Basan Seon Ju Yeo Hyun Park |
| author_facet | Bao Tuan Duong Duc Duong Than Ulaankhuu Ankhanbaatar Delgerzul Gombo-Ochir Gansukh Shura Amartuvshin Tsolmon Chris Ka Pun Mok Ganzorig Basan Seon Ju Yeo Hyun Park |
| author_sort | Bao Tuan Duong |
| collection | DOAJ |
| description | Several novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia in 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two novel genotypes of A(H5N6) subtype in Mongolia during 2018–2019 (A/wildDuck/MN/H5N6/2018-19). Their evolution pattern and molecular characteristics were evaluated using gene sequencing and their pathogenicity was determined using a mouse model. We also compared their antigenicity with previous H5 Clade 2.3.4.4 human isolates by cross-hemagglutination inhibition (HI). Our data suggests that A/wildDuck/MN/H5N6/2018-19 belongs to clade 2.3.4.4h, and maintains several residues associated with mammal adaptation. In addition, our evaluations revealed that their isolates are less virulent in mice than the previously identified H5 human isolates. However, their antigenicity is distinct from other HPAIVs H5 clade 2.3.4.4, thus supporting their continued evaluation as potential infection risks and the preparation of novel candidate vaccines for their neutralization. |
| first_indexed | 2024-04-12T17:40:59Z |
| format | Article |
| id | doaj.art-f6c6f1f62b9a4662aa0c5b51b54d0ef3 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-12T17:40:59Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-f6c6f1f62b9a4662aa0c5b51b54d0ef32022-12-22T03:22:48ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512022-12-011111425143410.1080/22221751.2022.2069515Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse modelBao Tuan Duong0Duc Duong Than1Ulaankhuu Ankhanbaatar2Delgerzul Gombo-Ochir3Gansukh Shura4Amartuvshin Tsolmon5Chris Ka Pun Mok6Ganzorig Basan7Seon Ju Yeo8Hyun Park9Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, KoreaZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, KoreaState Central Veterinary Laboratory, Zaisan, Ulaanbaatar, MongoliaState Central Veterinary Laboratory, Zaisan, Ulaanbaatar, MongoliaState Central Veterinary Laboratory, Zaisan, Ulaanbaatar, MongoliaState Central Veterinary Laboratory, Zaisan, Ulaanbaatar, MongoliaHKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaState Central Veterinary Laboratory, Zaisan, Ulaanbaatar, MongoliaDepartment of Tropical Medicine and Parasitology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, KoreaZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, KoreaSeveral novel highly pathogenic avian influenza (HPAIVs) A(H5N6) viruses were reported in Mongolia in 2020, some of which included host-specific markers associated with mammalian infection. However, their pathogenicity has not yet been investigated. Here, we isolated and evaluate two novel genotypes of A(H5N6) subtype in Mongolia during 2018–2019 (A/wildDuck/MN/H5N6/2018-19). Their evolution pattern and molecular characteristics were evaluated using gene sequencing and their pathogenicity was determined using a mouse model. We also compared their antigenicity with previous H5 Clade 2.3.4.4 human isolates by cross-hemagglutination inhibition (HI). Our data suggests that A/wildDuck/MN/H5N6/2018-19 belongs to clade 2.3.4.4h, and maintains several residues associated with mammal adaptation. In addition, our evaluations revealed that their isolates are less virulent in mice than the previously identified H5 human isolates. However, their antigenicity is distinct from other HPAIVs H5 clade 2.3.4.4, thus supporting their continued evaluation as potential infection risks and the preparation of novel candidate vaccines for their neutralization.https://www.tandfonline.com/doi/10.1080/22221751.2022.2069515Avian influenza virusesMongoliaH5N62.3.4.4hhemagglutination inhibition |
| spellingShingle | Bao Tuan Duong Duc Duong Than Ulaankhuu Ankhanbaatar Delgerzul Gombo-Ochir Gansukh Shura Amartuvshin Tsolmon Chris Ka Pun Mok Ganzorig Basan Seon Ju Yeo Hyun Park Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model Emerging Microbes and Infections Avian influenza viruses Mongolia H5N6 2.3.4.4h hemagglutination inhibition |
| title | Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model |
| title_full | Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model |
| title_fullStr | Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model |
| title_full_unstemmed | Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model |
| title_short | Assessing potential pathogenicity of novel highly pathogenic avian influenza (H5N6) viruses isolated from Mongolian wild duck feces using a mouse model |
| title_sort | assessing potential pathogenicity of novel highly pathogenic avian influenza h5n6 viruses isolated from mongolian wild duck feces using a mouse model |
| topic | Avian influenza viruses Mongolia H5N6 2.3.4.4h hemagglutination inhibition |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2022.2069515 |
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