Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
ABSTRACTBacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the devel...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2023.2172666 |
| _version_ | 1827303833486753792 |
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| author | Laurent Ferraris Aurélie Balvay Deborah Bellet Johanne Delannoy Claire Maudet Thibaut Larcher Jean-Christophe Rozé Catherine Philippe Thierry Meylheuc Marie-José Butel Sylvie Rabot Julio Aires |
| author_facet | Laurent Ferraris Aurélie Balvay Deborah Bellet Johanne Delannoy Claire Maudet Thibaut Larcher Jean-Christophe Rozé Catherine Philippe Thierry Meylheuc Marie-José Butel Sylvie Rabot Julio Aires |
| author_sort | Laurent Ferraris |
| collection | DOAJ |
| description | ABSTRACTBacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricum and Clostridium neonatale in NEC. First, we produced C.butyricum and C.neonatale strains impaired in butyrate production by genetically inactivating the hbd gene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies. |
| first_indexed | 2024-03-11T14:20:53Z |
| format | Article |
| id | doaj.art-f6c8c34fe93248af80243bd2a9923172 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T17:07:52Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-f6c8c34fe93248af80243bd2a99231722024-03-28T22:38:19ZengTaylor & Francis GroupGut Microbes1949-09761949-09842023-12-0115110.1080/19490976.2023.2172666Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicityLaurent Ferraris0Aurélie Balvay1Deborah Bellet2Johanne Delannoy3Claire Maudet4Thibaut Larcher5Jean-Christophe Rozé6Catherine Philippe7Thierry Meylheuc8Marie-José Butel9Sylvie Rabot10Julio Aires11Université Paris Cité, INSERM, UMR-S 1139, 3PHM, Paris, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris Cité, INSERM, UMR-S 1139, 3PHM, Paris, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceINRAE, Oniris, PAnTher, APEX, Nantes, FranceINRAE, UMR 1280, Physiologie des Adaptations Nutritionnelles (PhAN), Université hospitalière de Nantes, Nantes, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris Cité, INSERM, UMR-S 1139, 3PHM, Paris, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, FranceUniversité Paris Cité, INSERM, UMR-S 1139, 3PHM, Paris, FranceABSTRACTBacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricum and Clostridium neonatale in NEC. First, we produced C.butyricum and C.neonatale strains impaired in butyrate production by genetically inactivating the hbd gene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies.https://www.tandfonline.com/doi/10.1080/19490976.2023.2172666Clostridium butyricumClostridium neonatalenecrotizing enterocolitisbutyrateanimal modelbeta-hydroxybutyryl-CoA dehydrogenase |
| spellingShingle | Laurent Ferraris Aurélie Balvay Deborah Bellet Johanne Delannoy Claire Maudet Thibaut Larcher Jean-Christophe Rozé Catherine Philippe Thierry Meylheuc Marie-José Butel Sylvie Rabot Julio Aires Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity Gut Microbes Clostridium butyricum Clostridium neonatale necrotizing enterocolitis butyrate animal model beta-hydroxybutyryl-CoA dehydrogenase |
| title | Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity |
| title_full | Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity |
| title_fullStr | Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity |
| title_full_unstemmed | Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity |
| title_short | Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity |
| title_sort | neonatal necrotizing enterocolitis clostridium butyricum and clostridium neonatale fermentation metabolism and enteropathogenicity |
| topic | Clostridium butyricum Clostridium neonatale necrotizing enterocolitis butyrate animal model beta-hydroxybutyryl-CoA dehydrogenase |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2023.2172666 |
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