Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme
Objective To investigate the expression and clinical significance of ZMYND11 in glioblastoma multiforme(GBM). Methods The tumor samples(tumor group) and non-neoplasm brain tissue(control group) were collected intraoperatively from 20 cases of GBM and 20 patients with severe brain injury in the Secon...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2018-08-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.1362.htm |
| _version_ | 1818265167713009664 |
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| author | YANG Jipeng QIU Xiang LU Shengkui SUN Xiaofeng REN Xiaofan YANG Jiankai SHANG Hongbo LYU Dongsheng GENG Shaomei |
| author_facet | YANG Jipeng QIU Xiang LU Shengkui SUN Xiaofeng REN Xiaofan YANG Jiankai SHANG Hongbo LYU Dongsheng GENG Shaomei |
| author_sort | YANG Jipeng |
| collection | DOAJ |
| description | Objective To investigate the expression and clinical significance of ZMYND11 in glioblastoma multiforme(GBM). Methods The tumor samples(tumor group) and non-neoplasm brain tissue(control group) were collected intraoperatively from 20 cases of GBM and 20 patients with severe brain injury in the Second Hospital of Hebei Medical University. The difference of ZMYND11 expression between two groups was determined using qRT-PCR and Western blot. U87 cells with upregulated ZMYND11 expression was constructed using transfection of ZMYND11-overexpression lentivirus. CCK, Transwell assay and flow cytometry were performed to identify the role of ZMYND11 in the proliferation, invasion and apoptosis of U87 cells. U87 cells with ZMYND11 overexpression were inoculated into nude mice for in vivo experiment. Results The expression levels of ZMYND11 mRNA and protein were lower in GBM tissues than those in non-neoplasm brain tissues(P < 0.001). In vitro, ZMYND11 inhibited effectively the proliferation and invasion, as well as promoted the apoptosis of U87 cells. In vivo, ZMYND11 suppressed remarkably the growth of graft tumor. Conclusion ZMYND11 inhibits the development and progression of GBM. |
| first_indexed | 2024-12-12T19:46:30Z |
| format | Article |
| id | doaj.art-f6cd6ad5ee6e493cb1b810e084f6871a |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-12T19:46:30Z |
| publishDate | 2018-08-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-f6cd6ad5ee6e493cb1b810e084f6871a2022-12-22T00:14:05ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-08-0145856056510.3971/j.issn.1000-8578.2018.17.13628578.2018.17.1362Expression and Clinical Significance of ZMYND11 in Glioblastoma MultiformeYANG Jipeng0QIU Xiang1LU Shengkui2SUN Xiaofeng3REN Xiaofan4YANG Jiankai5SHANG Hongbo6LYU Dongsheng7GENG Shaomei8Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of General Practice, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, Yuanshi County Hospital of Traditional Chinese Medicine, Shijiazhuang 051130, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaObjective To investigate the expression and clinical significance of ZMYND11 in glioblastoma multiforme(GBM). Methods The tumor samples(tumor group) and non-neoplasm brain tissue(control group) were collected intraoperatively from 20 cases of GBM and 20 patients with severe brain injury in the Second Hospital of Hebei Medical University. The difference of ZMYND11 expression between two groups was determined using qRT-PCR and Western blot. U87 cells with upregulated ZMYND11 expression was constructed using transfection of ZMYND11-overexpression lentivirus. CCK, Transwell assay and flow cytometry were performed to identify the role of ZMYND11 in the proliferation, invasion and apoptosis of U87 cells. U87 cells with ZMYND11 overexpression were inoculated into nude mice for in vivo experiment. Results The expression levels of ZMYND11 mRNA and protein were lower in GBM tissues than those in non-neoplasm brain tissues(P < 0.001). In vitro, ZMYND11 inhibited effectively the proliferation and invasion, as well as promoted the apoptosis of U87 cells. In vivo, ZMYND11 suppressed remarkably the growth of graft tumor. Conclusion ZMYND11 inhibits the development and progression of GBM.http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.1362.htmglioblastoma multiformecell proliferationcell apoptosisnude mouse transplantation tumor |
| spellingShingle | YANG Jipeng QIU Xiang LU Shengkui SUN Xiaofeng REN Xiaofan YANG Jiankai SHANG Hongbo LYU Dongsheng GENG Shaomei Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme Zhongliu Fangzhi Yanjiu glioblastoma multiforme cell proliferation cell apoptosis nude mouse transplantation tumor |
| title | Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme |
| title_full | Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme |
| title_fullStr | Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme |
| title_full_unstemmed | Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme |
| title_short | Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme |
| title_sort | expression and clinical significance of zmynd11 in glioblastoma multiforme |
| topic | glioblastoma multiforme cell proliferation cell apoptosis nude mouse transplantation tumor |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.1362.htm |
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