Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
The difference between early pregnancy and delivery rate is quite large in assisted reproduction techniques (ARTs), including animal cloning. However, it is not clear why the implanted fetuses aborted after the early pregnancy stage. In the present study, we tried to evaluate the developmental and m...
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MDPI AG
2020-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/622 |
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| author | In-Sul Hwang Mi-Ryung Park Hae-Sun Lee Tae-Uk Kwak Hwa-Young Son Jong-Koo Kang Jeong-Woong Lee Kichoon Lee Eung-Woo Park Seongsoo Hwang |
| author_facet | In-Sul Hwang Mi-Ryung Park Hae-Sun Lee Tae-Uk Kwak Hwa-Young Son Jong-Koo Kang Jeong-Woong Lee Kichoon Lee Eung-Woo Park Seongsoo Hwang |
| author_sort | In-Sul Hwang |
| collection | DOAJ |
| description | The difference between early pregnancy and delivery rate is quite large in assisted reproduction techniques (ARTs), including animal cloning. However, it is not clear why the implanted fetuses aborted after the early pregnancy stage. In the present study, we tried to evaluate the developmental and morphological characteristics of porcine parthenogenetically activated (PA) embryos or fetuses by electric stimulation during the early pregnancy period. The implanted PA and artificially inseminated (AI) embryos and fetuses were collected at day 26 and 35 after embryo transfer, respectively. The developmental and morphological parameters in the PA embryos at day 26 were similar to the AI embryos. The size, weight, formation of major organs, and apoptotic cells were not statistically different in both embryos at day 26. However, the PA fetuses at day 35 showed ceased fetal development and degenerated with abnormal morphologies in their organs. The day 35 PA fetuses showed significantly higher apoptotic cells and lower methylation status in three differentially methylated regions of the H19 gene compared to their comparators. Therefore, the normal development of PA embryos and fetuses during early gestation could lead to these pregnancies being misinterpreted as normal and become one of the main reasons for the gap between early pregnancy and delivery rate. |
| first_indexed | 2024-03-10T20:41:32Z |
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| id | doaj.art-f6cd8d9b08414d19a4d405ca8ecaa8c8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:41:32Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f6cd8d9b08414d19a4d405ca8ecaa8c82023-11-19T20:42:17ZengMDPI AGAnimals2076-26152020-04-0110462210.3390/ani10040622Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early PregnancyIn-Sul Hwang0Mi-Ryung Park1Hae-Sun Lee2Tae-Uk Kwak3Hwa-Young Son4Jong-Koo Kang5Jeong-Woong Lee6Kichoon Lee7Eung-Woo Park8Seongsoo Hwang9Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, KoreaBiotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, KoreaDepartment of Animal Sciences, The Ohio State University, Columbus, OH 43210, USAAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaAnimal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, KoreaThe difference between early pregnancy and delivery rate is quite large in assisted reproduction techniques (ARTs), including animal cloning. However, it is not clear why the implanted fetuses aborted after the early pregnancy stage. In the present study, we tried to evaluate the developmental and morphological characteristics of porcine parthenogenetically activated (PA) embryos or fetuses by electric stimulation during the early pregnancy period. The implanted PA and artificially inseminated (AI) embryos and fetuses were collected at day 26 and 35 after embryo transfer, respectively. The developmental and morphological parameters in the PA embryos at day 26 were similar to the AI embryos. The size, weight, formation of major organs, and apoptotic cells were not statistically different in both embryos at day 26. However, the PA fetuses at day 35 showed ceased fetal development and degenerated with abnormal morphologies in their organs. The day 35 PA fetuses showed significantly higher apoptotic cells and lower methylation status in three differentially methylated regions of the H19 gene compared to their comparators. Therefore, the normal development of PA embryos and fetuses during early gestation could lead to these pregnancies being misinterpreted as normal and become one of the main reasons for the gap between early pregnancy and delivery rate.https://www.mdpi.com/2076-2615/10/4/622assisted parthenogenesispregnancyfetal developmentabortion |
| spellingShingle | In-Sul Hwang Mi-Ryung Park Hae-Sun Lee Tae-Uk Kwak Hwa-Young Son Jong-Koo Kang Jeong-Woong Lee Kichoon Lee Eung-Woo Park Seongsoo Hwang Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy Animals assisted parthenogenesis pregnancy fetal development abortion |
| title | Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy |
| title_full | Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy |
| title_fullStr | Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy |
| title_full_unstemmed | Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy |
| title_short | Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy |
| title_sort | developmental and degenerative characterization of porcine parthenogenetic fetuses during early pregnancy |
| topic | assisted parthenogenesis pregnancy fetal development abortion |
| url | https://www.mdpi.com/2076-2615/10/4/622 |
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