Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance
Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3...
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MDPI AG
2022-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/4/800 |
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| author | Rajaprakasam Sudhagar Shanmugavel Priyanka Vanniarajan Chockalingam Vaithiyanathan Sendhilvel Jegadeesan Souframanien Kalimuthu Raja Selvaraju Kanagarajan |
| author_facet | Rajaprakasam Sudhagar Shanmugavel Priyanka Vanniarajan Chockalingam Vaithiyanathan Sendhilvel Jegadeesan Souframanien Kalimuthu Raja Selvaraju Kanagarajan |
| author_sort | Rajaprakasam Sudhagar |
| collection | DOAJ |
| description | Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3 to 4). The PM altered the chlorophyll fluorescence (a/b ratio), maturity duration, and yield attributing traits. The yield loss ranged from 4.55% to 72.66%. After affirming the resistance level, the resistant mutant (RM) with minimum yield loss (scale:0) and the susceptible mutant (SM) with maximum loss (scale:4) were used for metabolomic analysis through GC-MS. PM infection induced expression of 66 metabolites representing 32 functional classes. The number of unique classes in RM and SM was 13 and 11, respectively, while eight were common. A fold change in the common metabolites indicated an enhanced accumulation of amine, alcohol, and ester in RM. Along with pathogen-induced defensive metabolites, RM produced silane and fluorene, whose biological significance in disease resistance is unknown. Though SM expressed defence-related bio-molecules, it failed to yield better. |
| first_indexed | 2024-03-09T11:18:44Z |
| format | Article |
| id | doaj.art-f6d51afa1ad84fffb468c9fe78c2794c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T11:18:44Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-f6d51afa1ad84fffb468c9fe78c2794c2023-12-01T00:26:08ZengMDPI AGAgronomy2073-43952022-03-0112480010.3390/agronomy12040800Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew ResistanceRajaprakasam Sudhagar0Shanmugavel Priyanka1Vanniarajan Chockalingam2Vaithiyanathan Sendhilvel3Jegadeesan Souframanien4Kalimuthu Raja5Selvaraju Kanagarajan6Sugarcane Research Station, Tamil Nadu Agricultural University, Vellore 635806, Tamil Nadu, IndiaCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Plant Breeding and Genetics, Agricultural College & Research Institute, Agricultural University, Madurai 625104, Tamil Nadu, IndiaDepartment of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaBhabha Atomic Research Centre, Government of India, Trombay, Mumbai 400085, Maharashtra, IndiaDepartment of Nanoscience and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, 234 22 Lomma, SwedenHorse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3 to 4). The PM altered the chlorophyll fluorescence (a/b ratio), maturity duration, and yield attributing traits. The yield loss ranged from 4.55% to 72.66%. After affirming the resistance level, the resistant mutant (RM) with minimum yield loss (scale:0) and the susceptible mutant (SM) with maximum loss (scale:4) were used for metabolomic analysis through GC-MS. PM infection induced expression of 66 metabolites representing 32 functional classes. The number of unique classes in RM and SM was 13 and 11, respectively, while eight were common. A fold change in the common metabolites indicated an enhanced accumulation of amine, alcohol, and ester in RM. Along with pathogen-induced defensive metabolites, RM produced silane and fluorene, whose biological significance in disease resistance is unknown. Though SM expressed defence-related bio-molecules, it failed to yield better.https://www.mdpi.com/2073-4395/12/4/800<i>E. polygoni</i>GC-MShorse grammetabolomics |
| spellingShingle | Rajaprakasam Sudhagar Shanmugavel Priyanka Vanniarajan Chockalingam Vaithiyanathan Sendhilvel Jegadeesan Souframanien Kalimuthu Raja Selvaraju Kanagarajan Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance Agronomy <i>E. polygoni</i> GC-MS horse gram metabolomics |
| title | Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance |
| title_full | Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance |
| title_fullStr | Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance |
| title_full_unstemmed | Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance |
| title_short | Development and Metabolic Characterization of Horse Gram (<i>Macrotyloma uniflorum</i> Lam. (Verdc.)) Mutants for Powdery Mildew Resistance |
| title_sort | development and metabolic characterization of horse gram i macrotyloma uniflorum i lam verdc mutants for powdery mildew resistance |
| topic | <i>E. polygoni</i> GC-MS horse gram metabolomics |
| url | https://www.mdpi.com/2073-4395/12/4/800 |
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