The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol
Cervical cancer is a global health concern. Persistent oncogenic human papillomavirus (HPV) infection is a prerequisite for the development of cervical cancer. Emerging research indicates that the vaginal microbiota may play both protective and harmful roles in the acquisition and persistence of HPV...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Medicine in Microecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590097822000222 |
| _version_ | 1797900668975972352 |
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| author | Zuyi Zhou Yi Hou Wei Qing Yiya Shi Yingxuan Zhang Rongdan Chen Jinxia Ou Hongwei Zhou Muxuan Chen |
| author_facet | Zuyi Zhou Yi Hou Wei Qing Yiya Shi Yingxuan Zhang Rongdan Chen Jinxia Ou Hongwei Zhou Muxuan Chen |
| author_sort | Zuyi Zhou |
| collection | DOAJ |
| description | Cervical cancer is a global health concern. Persistent oncogenic human papillomavirus (HPV) infection is a prerequisite for the development of cervical cancer. Emerging research indicates that the vaginal microbiota may play both protective and harmful roles in the acquisition and persistence of HPV and the development of cervical cancer. This multicenter cohort study mainly aims to reveal the association of vaginal microbiota with the outcome of HPV infection in six months in women of reproductive age. We will recruit 50 research centers and enroll a total of about 10,000 female volunteers with a series of strict inclusion and exclusion criteria across China, including HPV positive and HPV negative women. A unified questionnaire will be used to obtain the sociodemographic information, clinical data and lifestyle of all volunteers. Specimens including vaginal secretions swabs and cervical exfoliated cells will be collected at the first visit and follow-up after six months. We will use 16S rRNA sequencing to characterize the vaginal microbiota of volunteers’ vaginal swabs. Twenty-one HPV genotypes and other sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and Herpes simplex virus type Ⅱ will be examined using flow-through hybridization and gene chip. The association of HPV infection and vaginal microbiota will be investigated, and the core microbiota attributes to the persistent HPV infection will be analyzed in this study. |
| first_indexed | 2024-04-10T08:49:40Z |
| format | Article |
| id | doaj.art-f6d5b2894e004ce89f2f2c175bd22d87 |
| institution | Directory Open Access Journal |
| issn | 2590-0978 |
| language | English |
| last_indexed | 2024-04-10T08:49:40Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Microecology |
| spelling | doaj.art-f6d5b2894e004ce89f2f2c175bd22d872023-02-22T04:32:11ZengElsevierMedicine in Microecology2590-09782023-03-0115100072The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocolZuyi Zhou0Yi Hou1Wei Qing2Yiya Shi3Yingxuan Zhang4Rongdan Chen5Jinxia Ou6Hongwei Zhou7Muxuan Chen8Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Corresponding author. Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China; Corresponding author. Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.Cervical cancer is a global health concern. Persistent oncogenic human papillomavirus (HPV) infection is a prerequisite for the development of cervical cancer. Emerging research indicates that the vaginal microbiota may play both protective and harmful roles in the acquisition and persistence of HPV and the development of cervical cancer. This multicenter cohort study mainly aims to reveal the association of vaginal microbiota with the outcome of HPV infection in six months in women of reproductive age. We will recruit 50 research centers and enroll a total of about 10,000 female volunteers with a series of strict inclusion and exclusion criteria across China, including HPV positive and HPV negative women. A unified questionnaire will be used to obtain the sociodemographic information, clinical data and lifestyle of all volunteers. Specimens including vaginal secretions swabs and cervical exfoliated cells will be collected at the first visit and follow-up after six months. We will use 16S rRNA sequencing to characterize the vaginal microbiota of volunteers’ vaginal swabs. Twenty-one HPV genotypes and other sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and Herpes simplex virus type Ⅱ will be examined using flow-through hybridization and gene chip. The association of HPV infection and vaginal microbiota will be investigated, and the core microbiota attributes to the persistent HPV infection will be analyzed in this study.http://www.sciencedirect.com/science/article/pii/S2590097822000222Vaginal microbiotaHuman papillomavirus infection16S rRNA sequencingMulticenter cohort study |
| spellingShingle | Zuyi Zhou Yi Hou Wei Qing Yiya Shi Yingxuan Zhang Rongdan Chen Jinxia Ou Hongwei Zhou Muxuan Chen The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol Medicine in Microecology Vaginal microbiota Human papillomavirus infection 16S rRNA sequencing Multicenter cohort study |
| title | The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol |
| title_full | The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol |
| title_fullStr | The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol |
| title_full_unstemmed | The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol |
| title_short | The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol |
| title_sort | association of hpv infection and vaginal microbiota of reproductive women in china a multicenter cohort study protocol |
| topic | Vaginal microbiota Human papillomavirus infection 16S rRNA sequencing Multicenter cohort study |
| url | http://www.sciencedirect.com/science/article/pii/S2590097822000222 |
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