Diversity of plant defense elicitor peptides within the Rosaceae
Abstract Background Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by bacteria, fungi and herbivores. Pep topic application and transgenic ex...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-01-01
|
| Series: | BMC Genetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12863-017-0593-4 |
| _version_ | 1818264839335706624 |
|---|---|
| author | Cristina Ruiz Anna Nadal Laura Foix Laura Montesinos Emilio Montesinos Maria Pla |
| author_facet | Cristina Ruiz Anna Nadal Laura Foix Laura Montesinos Emilio Montesinos Maria Pla |
| author_sort | Cristina Ruiz |
| collection | DOAJ |
| description | Abstract Background Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by bacteria, fungi and herbivores. Pep topic application and transgenic expression have been found to enhance disease resistance in a small number of model plant-pathogen systems. The action of Peps relies on perception by specific receptors, so displaying a family-specific activity. Recently, the presence and activity of Peps within the Rosaceae has been demonstrated. Here we characterized the population of Pep sequences within the economically important plant family of Rosaceae, with special emphasis on the Amygdaleae and Pyreae tribes, which include the most relevant edible species such as apple, pear and peach, and numerous ornamental and wild species (e.g. photinia, firethorn and hawthorn). Results The systematic experimental search for Pep and the corresponding precursor PROPEP sequences within 36 Amygdaleae and Pyreae species, and 100 cultivars had a highly homogeneous pattern, with two tribe-specific Pep types per plant, i.e. Pep1 and Pep2 (Amygdaleae) or Pep3 and Pep4 (Pyreae). Pep2 and Pep3 are highly conserved, reaching identity percentages similar to those of genes used in plant phylogenetic analyses, while Pep1 and Pep4 are somewhat more variable, with similar values to the corresponding PROPEPs. In contrast to Pep3 and Pep4, Pep1 and Pep2 sequences of different species paralleled their phylogenetic relationships, and putative ancestor sequences were identified. The large amount of sequences allowed refining of a C-terminal consensus sequence that would support the protective activity of Pep1–4 in a Prunus spp. and Xanthomonas arboricola pv. pruni system. Moreover, tribe-specific consensus sequences were deduced at the center and C-terminal regions of Peps, which might explain the higher protection efficiencies described upon topic treatments with Peps from the same tribe. Conclusions The present study substantially enhances the knowledge on Peps within the Amygdaleae and Pyreae species. It can be the basis to design and fine-tune new control tools against important plant pathogens affecting Prunus, Pyrus and Malus species. |
| first_indexed | 2024-12-12T19:41:17Z |
| format | Article |
| id | doaj.art-f6d684784746420abd8bbeb85fb3ad6d |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T19:41:17Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-f6d684784746420abd8bbeb85fb3ad6d2022-12-22T00:14:13ZengBMCBMC Genetics1471-21562018-01-0119111210.1186/s12863-017-0593-4Diversity of plant defense elicitor peptides within the RosaceaeCristina Ruiz0Anna Nadal1Laura Foix2Laura Montesinos3Emilio Montesinos4Maria Pla5Institute for Food and Agricultural Technology (INTEA), University of GironaInstitute for Food and Agricultural Technology (INTEA), University of GironaInstitute for Food and Agricultural Technology (INTEA), University of GironaInstitute for Food and Agricultural Technology (INTEA), University of GironaInstitute for Food and Agricultural Technology (INTEA), University of GironaInstitute for Food and Agricultural Technology (INTEA), University of GironaAbstract Background Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by bacteria, fungi and herbivores. Pep topic application and transgenic expression have been found to enhance disease resistance in a small number of model plant-pathogen systems. The action of Peps relies on perception by specific receptors, so displaying a family-specific activity. Recently, the presence and activity of Peps within the Rosaceae has been demonstrated. Here we characterized the population of Pep sequences within the economically important plant family of Rosaceae, with special emphasis on the Amygdaleae and Pyreae tribes, which include the most relevant edible species such as apple, pear and peach, and numerous ornamental and wild species (e.g. photinia, firethorn and hawthorn). Results The systematic experimental search for Pep and the corresponding precursor PROPEP sequences within 36 Amygdaleae and Pyreae species, and 100 cultivars had a highly homogeneous pattern, with two tribe-specific Pep types per plant, i.e. Pep1 and Pep2 (Amygdaleae) or Pep3 and Pep4 (Pyreae). Pep2 and Pep3 are highly conserved, reaching identity percentages similar to those of genes used in plant phylogenetic analyses, while Pep1 and Pep4 are somewhat more variable, with similar values to the corresponding PROPEPs. In contrast to Pep3 and Pep4, Pep1 and Pep2 sequences of different species paralleled their phylogenetic relationships, and putative ancestor sequences were identified. The large amount of sequences allowed refining of a C-terminal consensus sequence that would support the protective activity of Pep1–4 in a Prunus spp. and Xanthomonas arboricola pv. pruni system. Moreover, tribe-specific consensus sequences were deduced at the center and C-terminal regions of Peps, which might explain the higher protection efficiencies described upon topic treatments with Peps from the same tribe. Conclusions The present study substantially enhances the knowledge on Peps within the Amygdaleae and Pyreae species. It can be the basis to design and fine-tune new control tools against important plant pathogens affecting Prunus, Pyrus and Malus species.http://link.springer.com/article/10.1186/s12863-017-0593-4Plant elicitor peptide (pep)PROPEPPlant defenseRosaceaePrunusPyreae |
| spellingShingle | Cristina Ruiz Anna Nadal Laura Foix Laura Montesinos Emilio Montesinos Maria Pla Diversity of plant defense elicitor peptides within the Rosaceae BMC Genetics Plant elicitor peptide (pep) PROPEP Plant defense Rosaceae Prunus Pyreae |
| title | Diversity of plant defense elicitor peptides within the Rosaceae |
| title_full | Diversity of plant defense elicitor peptides within the Rosaceae |
| title_fullStr | Diversity of plant defense elicitor peptides within the Rosaceae |
| title_full_unstemmed | Diversity of plant defense elicitor peptides within the Rosaceae |
| title_short | Diversity of plant defense elicitor peptides within the Rosaceae |
| title_sort | diversity of plant defense elicitor peptides within the rosaceae |
| topic | Plant elicitor peptide (pep) PROPEP Plant defense Rosaceae Prunus Pyreae |
| url | http://link.springer.com/article/10.1186/s12863-017-0593-4 |
| work_keys_str_mv | AT cristinaruiz diversityofplantdefenseelicitorpeptideswithintherosaceae AT annanadal diversityofplantdefenseelicitorpeptideswithintherosaceae AT laurafoix diversityofplantdefenseelicitorpeptideswithintherosaceae AT lauramontesinos diversityofplantdefenseelicitorpeptideswithintherosaceae AT emiliomontesinos diversityofplantdefenseelicitorpeptideswithintherosaceae AT mariapla diversityofplantdefenseelicitorpeptideswithintherosaceae |