Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis
<p>Identification of protein residues from prehistoric cooking pottery using mass spectrometry is challenging because proteins are removed from original tissues, are degraded from cooking, may be poorly preserved due to diagenesis, and occur in a palimpsest of exogenous soil proteins. In contr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Ethnobiology
2015-09-01
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| Series: | Ethnobiology Letters |
| Subjects: | |
| Online Access: | http://ojs.ethnobiology.org/index.php/ebl/article/view/294 |
| _version_ | 1818886748305883136 |
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| author | Andrew Barker Jonathan Dombrosky Dale Chaput Barney Venbles Steve Wolverton Stanley M. Stevens |
| author_facet | Andrew Barker Jonathan Dombrosky Dale Chaput Barney Venbles Steve Wolverton Stanley M. Stevens |
| author_sort | Andrew Barker |
| collection | DOAJ |
| description | <p>Identification of protein residues from prehistoric cooking pottery using mass spectrometry is challenging because proteins are removed from original tissues, are degraded from cooking, may be poorly preserved due to diagenesis, and occur in a palimpsest of exogenous soil proteins. In contrast, bone proteins are abundant and well preserved. This research is part of a larger method-development project for innovation and improvement of liquid chromatography – mass spectrometry analysis of protein residues from cooking pottery; here we validate the potential of our extraction and characterization approach via application to ancient bone proteins. Because of its preservation potential for proteins and given that our approach is destructive, ancient bone identified via skeletal morphology represents an appropriate verification target. Proteins were identified from zooarchaeological turkey (<em>Meleagris gallopavo </em>Linnaeus Phasianidae), rabbit (Lagomorpha), and squirrel (Sciuridae) remains excavated from ancient pueblo archaeological sites in southwestern Colorado using a non-targeted LC-MS/MS approach. The data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD002440. Improvement of highly sensitive targeted LC-MS/MS approaches is an avenue for future method development related to the study of protein residues from artifacts such as stone tools and pottery.</p> |
| first_indexed | 2024-12-19T16:26:16Z |
| format | Article |
| id | doaj.art-f6d90f559dbc430c9ead4fb1051dfc8d |
| institution | Directory Open Access Journal |
| issn | 2159-8126 |
| language | English |
| last_indexed | 2024-12-19T16:26:16Z |
| publishDate | 2015-09-01 |
| publisher | Society of Ethnobiology |
| record_format | Article |
| series | Ethnobiology Letters |
| spelling | doaj.art-f6d90f559dbc430c9ead4fb1051dfc8d2022-12-21T20:14:19ZengSociety of EthnobiologyEthnobiology Letters2159-81262015-09-016116217410.14237/ebl.6.1.2015.294158Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue AnalysisAndrew Barker0Jonathan Dombrosky1Dale Chaput2Barney Venbles3Steve Wolverton4Stanley M. Stevens5Department of Biological Sciences, University of North Texas, Denton, TX 76203.Department of Anthropology, University of New Mexico, Albuquerque, NM 87131.Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620Department of Biological Sciences, University of North Texas, Denton, TX 76203.Department of Geography, University of North Texas, Denton, TX 76203.Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620<p>Identification of protein residues from prehistoric cooking pottery using mass spectrometry is challenging because proteins are removed from original tissues, are degraded from cooking, may be poorly preserved due to diagenesis, and occur in a palimpsest of exogenous soil proteins. In contrast, bone proteins are abundant and well preserved. This research is part of a larger method-development project for innovation and improvement of liquid chromatography – mass spectrometry analysis of protein residues from cooking pottery; here we validate the potential of our extraction and characterization approach via application to ancient bone proteins. Because of its preservation potential for proteins and given that our approach is destructive, ancient bone identified via skeletal morphology represents an appropriate verification target. Proteins were identified from zooarchaeological turkey (<em>Meleagris gallopavo </em>Linnaeus Phasianidae), rabbit (Lagomorpha), and squirrel (Sciuridae) remains excavated from ancient pueblo archaeological sites in southwestern Colorado using a non-targeted LC-MS/MS approach. The data have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD002440. Improvement of highly sensitive targeted LC-MS/MS approaches is an avenue for future method development related to the study of protein residues from artifacts such as stone tools and pottery.</p>http://ojs.ethnobiology.org/index.php/ebl/article/view/294Archaeological chemistryBone protein residuesAncient proteinsMass spectrometry |
| spellingShingle | Andrew Barker Jonathan Dombrosky Dale Chaput Barney Venbles Steve Wolverton Stanley M. Stevens Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis Ethnobiology Letters Archaeological chemistry Bone protein residues Ancient proteins Mass spectrometry |
| title | Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis |
| title_full | Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis |
| title_fullStr | Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis |
| title_full_unstemmed | Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis |
| title_short | Validation of a Non-Targeted LC-MS Approach for Identifying Ancient Proteins: Method Development on Bone to Improve Artifact Residue Analysis |
| title_sort | validation of a non targeted lc ms approach for identifying ancient proteins method development on bone to improve artifact residue analysis |
| topic | Archaeological chemistry Bone protein residues Ancient proteins Mass spectrometry |
| url | http://ojs.ethnobiology.org/index.php/ebl/article/view/294 |
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