Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders
Abstract Background Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main proble...
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BMC
2020-07-01
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| Series: | Molecular Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s10020-020-00192-0 |
| _version_ | 1818807492852842496 |
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| author | Aleksandra Skubis-Sikora Bartosz Sikora Agnieszka Witkowska Urszula Mazurek Joanna Gola |
| author_facet | Aleksandra Skubis-Sikora Bartosz Sikora Agnieszka Witkowska Urszula Mazurek Joanna Gola |
| author_sort | Aleksandra Skubis-Sikora |
| collection | DOAJ |
| description | Abstract Background Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main problem of somatic stem cells is a documented influence of various diseases, drugs or age which can inhibit cells activity. Therefore, in the present study, we investigated the influence of insulin resistance (IR) and type 2 diabetes (T2D) on the proliferation and differentiation potential of ADSCs. Methods The fat from subcutaneous abdominal adipose tissue was acquired by lipoaspiration from 23 voluntary participants, divided into three groups: with diabetes type 2, with insulin resistance and control healthy donors. The proliferative potential was analyzed by cell cytotoxicity assays and by mRNA expression of genes connected with proliferation. Flow cytometry was done for identifying proteins characteristic for mesenchymal stem cells and an analysis of osteogenic differentiation potential based on the assessment of osteogenic markers by real time RT-qPCR, and the evaluation of calcium deposition were also performed. Results The results showed that diabetes type 2 lowered the activity of ADSCs in proliferation assays and changed their phenotypical characteristics. Interestingly, we observed differences in the proliferation potential of ADSCs in patients with insulin resistance, which is often the first phase of diabetes, compared to the control. It might suggest that insulin resistance, early-stage T2D, alters the activity of cells. Moreover, expression of osteogenesis markers was higher in cells from T2D patients than in cells from patients with IR and control. Conclusion We conclude that type 2 diabetes changes the activity of stem cells, and insulin resistance influences on the proliferation of ADSCs. |
| first_indexed | 2024-12-18T19:26:32Z |
| format | Article |
| id | doaj.art-f6dd014f235d465ab3aba714c4091661 |
| institution | Directory Open Access Journal |
| issn | 1076-1551 1528-3658 |
| language | English |
| last_indexed | 2024-12-18T19:26:32Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj.art-f6dd014f235d465ab3aba714c40916612022-12-21T20:55:51ZengBMCMolecular Medicine1076-15511528-36582020-07-012611910.1186/s10020-020-00192-0Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disordersAleksandra Skubis-Sikora0Bartosz Sikora1Agnieszka Witkowska2Urszula Mazurek3Joanna Gola4Department of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in KatowiceDepartment of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in KatowiceFundacja Zdrowego ŻyciaJózef Tyszkiewicz Higher School in Bielsko-Biała, ulDepartment of Molecular Biology, Chair of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in KatowiceAbstract Background Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main problem of somatic stem cells is a documented influence of various diseases, drugs or age which can inhibit cells activity. Therefore, in the present study, we investigated the influence of insulin resistance (IR) and type 2 diabetes (T2D) on the proliferation and differentiation potential of ADSCs. Methods The fat from subcutaneous abdominal adipose tissue was acquired by lipoaspiration from 23 voluntary participants, divided into three groups: with diabetes type 2, with insulin resistance and control healthy donors. The proliferative potential was analyzed by cell cytotoxicity assays and by mRNA expression of genes connected with proliferation. Flow cytometry was done for identifying proteins characteristic for mesenchymal stem cells and an analysis of osteogenic differentiation potential based on the assessment of osteogenic markers by real time RT-qPCR, and the evaluation of calcium deposition were also performed. Results The results showed that diabetes type 2 lowered the activity of ADSCs in proliferation assays and changed their phenotypical characteristics. Interestingly, we observed differences in the proliferation potential of ADSCs in patients with insulin resistance, which is often the first phase of diabetes, compared to the control. It might suggest that insulin resistance, early-stage T2D, alters the activity of cells. Moreover, expression of osteogenesis markers was higher in cells from T2D patients than in cells from patients with IR and control. Conclusion We conclude that type 2 diabetes changes the activity of stem cells, and insulin resistance influences on the proliferation of ADSCs.http://link.springer.com/article/10.1186/s10020-020-00192-0MetforminMesenchymal stem cellsMetabolic impairment, type 2 diabetes, insulin resistance |
| spellingShingle | Aleksandra Skubis-Sikora Bartosz Sikora Agnieszka Witkowska Urszula Mazurek Joanna Gola Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders Molecular Medicine Metformin Mesenchymal stem cells Metabolic impairment, type 2 diabetes, insulin resistance |
| title | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
| title_full | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
| title_fullStr | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
| title_full_unstemmed | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
| title_short | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
| title_sort | osteogenesis of adipose derived stem cells from patients with glucose metabolism disorders |
| topic | Metformin Mesenchymal stem cells Metabolic impairment, type 2 diabetes, insulin resistance |
| url | http://link.springer.com/article/10.1186/s10020-020-00192-0 |
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