Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album
The natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses....
| Format: | Article |
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| Language: | English |
| Published: |
Iranian Medicinal Plants Society
2013-09-01
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| Series: | Journal of Medicinal Plants and By-products |
| Subjects: | |
| Online Access: | http://jmpb.areeo.ac.ir/article_108495_ea74e2d08e7221c9c7cc339b8cb799dc.pdf |
| _version_ | 1819203313484169216 |
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| collection | DOAJ |
| description | The natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. In order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect of different concentrations of α-Naphthalene acetic acid (NAA) (0, 0.4 and 1 mg/l), Kinetin (Kin) (0, 0.2 and 0.4 mg/l) and combinations of NAA and Kin [(0.4 and 0.2), (0.4 and 0.4), (1 and 0.2) and (1 and 0.4) mg/l] on growth and accumulation of podophyllotoxin in callus, cell culture and in vitro propagated plantlets of Linum album was evaluated. The highest growth and accumulation of podophyllotoxin in callus culture was obtained when it grown under dark condition in comparison to photoperiod. The biomass of cell and callus do not changed under various hormone concentrations. Content of podophyllotoxin was enhanced in cell and callus cultures supplemented with NAA 1 mg/l + Kin 0.4 mg/l. The experiments demonstrated Kin 0.2 or 0.4 mg/l stimulated multiplication and production of podophyllotoxin in shoot culture. |
| first_indexed | 2024-12-23T04:17:56Z |
| format | Article |
| id | doaj.art-f6de7bebdd274bf280deaf039c807019 |
| institution | Directory Open Access Journal |
| issn | 2322-1399 2588-3739 |
| language | English |
| last_indexed | 2024-12-23T04:17:56Z |
| publishDate | 2013-09-01 |
| publisher | Iranian Medicinal Plants Society |
| record_format | Article |
| series | Journal of Medicinal Plants and By-products |
| spelling | doaj.art-f6de7bebdd274bf280deaf039c8070192022-12-21T18:00:20ZengIranian Medicinal Plants SocietyJournal of Medicinal Plants and By-products2322-13992588-37392013-09-01218389108495Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum albumThe natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. In order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect of different concentrations of α-Naphthalene acetic acid (NAA) (0, 0.4 and 1 mg/l), Kinetin (Kin) (0, 0.2 and 0.4 mg/l) and combinations of NAA and Kin [(0.4 and 0.2), (0.4 and 0.4), (1 and 0.2) and (1 and 0.4) mg/l] on growth and accumulation of podophyllotoxin in callus, cell culture and in vitro propagated plantlets of Linum album was evaluated. The highest growth and accumulation of podophyllotoxin in callus culture was obtained when it grown under dark condition in comparison to photoperiod. The biomass of cell and callus do not changed under various hormone concentrations. Content of podophyllotoxin was enhanced in cell and callus cultures supplemented with NAA 1 mg/l + Kin 0.4 mg/l. The experiments demonstrated Kin 0.2 or 0.4 mg/l stimulated multiplication and production of podophyllotoxin in shoot culture.http://jmpb.areeo.ac.ir/article_108495_ea74e2d08e7221c9c7cc339b8cb799dc.pdfPodophyllotoxinLinum albumα-Naphthalene acetic acidKinetin |
| spellingShingle | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album Journal of Medicinal Plants and By-products Podophyllotoxin Linum album α-Naphthalene acetic acid Kinetin |
| title | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album |
| title_full | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album |
| title_fullStr | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album |
| title_full_unstemmed | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album |
| title_short | Effect of Some Phytohormones on Podophyllotoxin Production in Cell and Plantlets Cultures of Linum album |
| title_sort | effect of some phytohormones on podophyllotoxin production in cell and plantlets cultures of linum album |
| topic | Podophyllotoxin Linum album α-Naphthalene acetic acid Kinetin |
| url | http://jmpb.areeo.ac.ir/article_108495_ea74e2d08e7221c9c7cc339b8cb799dc.pdf |