Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts
In dentofacial surgery, augmentation procedures employing xenografts have become a reliable treatment. Recent studies, however, have shown significant enhance-ments of the in vivo bone tissue augmentation using mesenchymal stem cells loaded into bone grafts. We conducted experimental and numerical i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2017-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2017-0169 |
| _version_ | 1797848747563024384 |
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| author | Ott Robert Wüstenhagen Carolin Martin Heiner Stiehm Michael Schmidt Wolfram Einnolf Nadia Frerich Bernhard Grabow Niels Schmitz Klaus-Peter Siewert Stefan |
| author_facet | Ott Robert Wüstenhagen Carolin Martin Heiner Stiehm Michael Schmidt Wolfram Einnolf Nadia Frerich Bernhard Grabow Niels Schmitz Klaus-Peter Siewert Stefan |
| author_sort | Ott Robert |
| collection | DOAJ |
| description | In dentofacial surgery, augmentation procedures employing xenografts have become a reliable treatment. Recent studies, however, have shown significant enhance-ments of the in vivo bone tissue augmentation using mesenchymal stem cells loaded into bone grafts. We conducted experimental and numerical investigations in flow perfusion systems to determine flow conditions which allow for homogenous stem cell distribution in BioOss Block (Geistlich Pharma AG, Switzerland) xenografts. Pressure gradient-velocity characteristics and flow distributions were investigated experimentally and numerically at steady state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.40. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa.s and a density of 998 kg/m3 was used. The geometry utilized in three-dimensional computa-tional fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT). Results of CFD analysis are in good accordance with experimental data. The comparison of the pressure gradient-velocity characteris-tics for experimental and numerical data yields a relative error of 3.6%. According to Darcy’s law for creeping fluid flow the experimentally determined permeability is 2.55.10-9 m2. Moreover, numerical flow distribution analysis shows an increasingly heterogenic streamline distribution for increasing Reynolds numbers. Experimentally validated CFD simulations introduced in this study provide a tool to assess optimal flow conditions for a homogenous stem cell distribution in perfusion flow systems. |
| first_indexed | 2024-04-09T18:32:33Z |
| format | Article |
| id | doaj.art-f6e2719d6a1f42b2a2e39eb5a25be77d |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-09T18:32:33Z |
| publishDate | 2017-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-f6e2719d6a1f42b2a2e39eb5a25be77d2023-04-11T17:07:14ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042017-09-013279980210.1515/cdbme-2017-0169cdbme-2017-0169Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenograftsOtt Robert0Wüstenhagen Carolin1Martin Heiner2Stiehm Michael3Schmidt Wolfram4Einnolf Nadia5Frerich Bernhard6Grabow Niels7Schmitz Klaus-Peter8Siewert Stefan9Institute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, GermanyInstitute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, GermanyInstitute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, GermanyRoweMed AG, Juri-Gagarin-Ring 4, 19370 Parchim, GermanyDepartment of Oral, Maxillofacial, and Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Friedrich-Barnewitz-Str. 4, 18119 Rostock, GermanyInstitute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, GermanyInstitute for ImplantTechnology and Biomaterials e.V., Friedrich-Barnewitz-Str.4, 18119 Rostock, GermanyIn dentofacial surgery, augmentation procedures employing xenografts have become a reliable treatment. Recent studies, however, have shown significant enhance-ments of the in vivo bone tissue augmentation using mesenchymal stem cells loaded into bone grafts. We conducted experimental and numerical investigations in flow perfusion systems to determine flow conditions which allow for homogenous stem cell distribution in BioOss Block (Geistlich Pharma AG, Switzerland) xenografts. Pressure gradient-velocity characteristics and flow distributions were investigated experimentally and numerically at steady state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.40. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa.s and a density of 998 kg/m3 was used. The geometry utilized in three-dimensional computa-tional fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT). Results of CFD analysis are in good accordance with experimental data. The comparison of the pressure gradient-velocity characteris-tics for experimental and numerical data yields a relative error of 3.6%. According to Darcy’s law for creeping fluid flow the experimentally determined permeability is 2.55.10-9 m2. Moreover, numerical flow distribution analysis shows an increasingly heterogenic streamline distribution for increasing Reynolds numbers. Experimentally validated CFD simulations introduced in this study provide a tool to assess optimal flow conditions for a homogenous stem cell distribution in perfusion flow systems.https://doi.org/10.1515/cdbme-2017-0169bone graftxenograftcomputational fluid dyna-micsdentofacial surgerystem cellperfusion flow system |
| spellingShingle | Ott Robert Wüstenhagen Carolin Martin Heiner Stiehm Michael Schmidt Wolfram Einnolf Nadia Frerich Bernhard Grabow Niels Schmitz Klaus-Peter Siewert Stefan Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts Current Directions in Biomedical Engineering bone graft xenograft computational fluid dyna-mics dentofacial surgery stem cell perfusion flow system |
| title | Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| title_full | Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| title_fullStr | Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| title_full_unstemmed | Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| title_short | Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| title_sort | experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts |
| topic | bone graft xenograft computational fluid dyna-mics dentofacial surgery stem cell perfusion flow system |
| url | https://doi.org/10.1515/cdbme-2017-0169 |
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