Metformin activity in an in vitro model of posterior capsule opacification
Purpose: To determine the activity of metformin in an in vitro model of posterior capsule opacification (PCO). Study Design: Experimental laboratory research. Methods: The HLE-B3 lens epithelial cell line was treated with PCO induction media (PCOM) supplemented with transforming growth factor-beta (...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | The Pan-American Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.thepajo.org/article.asp?issn=2666-4909;year=2018;volume=17;issue=4;spage=105;epage=112;aulast=Lasiste;type=0 |
| _version_ | 1818649482158407680 |
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| author | Jade Marie Lasiste Pablo Zoroquiain Denise Miyamoto Miguel N Burnier |
| author_facet | Jade Marie Lasiste Pablo Zoroquiain Denise Miyamoto Miguel N Burnier |
| author_sort | Jade Marie Lasiste |
| collection | DOAJ |
| description | Purpose: To determine the activity of metformin in an in vitro model of posterior capsule opacification (PCO).
Study Design: Experimental laboratory research.
Methods: The HLE-B3 lens epithelial cell line was treated with PCO induction media (PCOM) supplemented with transforming growth factor-beta (TGF-β) and fibro-blast growth factor (FGF). Different metformin concentrations (0-100 mM) were used. The following cellular parameters were assessed: (1) survival, using a viability assay; (2) morphology, via microscopy and image analysis; (3) migration, using the wound assay; (4) and expression of epithelial (Pax6, E-cadherin) and mesenchymal (α-smooth muscle actin or α-SMA, fibronectin) markers via Western blot. Expression of the uptake receptor SLC22A1 was evaluated in HLE-B3 and in human donor eyes with Western blot and immunohistochemistry, respectively. Statistical analysis of variance (ANOVA) with Tukey post-hoc test was done for analysis of cytotoxicity, morphology and migration data.
Results: Metformin was lethal to half (LC50) of the cells at 30 mM, and a decrease in viability (P<0.05) was noted at 5 mM. LECs in PCOM treated with 1 mM metformin showed increased Pax6 and E-cadherin and decreased α-SMA and fibronectin expression. LECs in PCOM treated with metformin also maintained epithelial morphology. Migration was inhibited with 0.5 mM metformin (P<0.05). Both HLE-B3 and the lens epithelium in donor eyes were found to express SLC22A1.
Conclusion: Metformin decreased survival and migration in LECs, maintaining epithelial phenotype and reducing mesenchymal marker expression. Metformin therefore has potential as an adjunct in PCO prevention. |
| first_indexed | 2024-12-17T01:35:01Z |
| format | Article |
| id | doaj.art-f6e53e009caf4da0b3913e25876db534 |
| institution | Directory Open Access Journal |
| issn | 2666-4909 2666-4909 |
| language | English |
| last_indexed | 2024-12-17T01:35:01Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Pan-American Journal of Ophthalmology |
| spelling | doaj.art-f6e53e009caf4da0b3913e25876db5342022-12-21T22:08:28ZengWolters Kluwer Medknow PublicationsThe Pan-American Journal of Ophthalmology2666-49092666-49092018-01-01174105112Metformin activity in an in vitro model of posterior capsule opacificationJade Marie LasistePablo ZoroquiainDenise MiyamotoMiguel N BurnierPurpose: To determine the activity of metformin in an in vitro model of posterior capsule opacification (PCO). Study Design: Experimental laboratory research. Methods: The HLE-B3 lens epithelial cell line was treated with PCO induction media (PCOM) supplemented with transforming growth factor-beta (TGF-β) and fibro-blast growth factor (FGF). Different metformin concentrations (0-100 mM) were used. The following cellular parameters were assessed: (1) survival, using a viability assay; (2) morphology, via microscopy and image analysis; (3) migration, using the wound assay; (4) and expression of epithelial (Pax6, E-cadherin) and mesenchymal (α-smooth muscle actin or α-SMA, fibronectin) markers via Western blot. Expression of the uptake receptor SLC22A1 was evaluated in HLE-B3 and in human donor eyes with Western blot and immunohistochemistry, respectively. Statistical analysis of variance (ANOVA) with Tukey post-hoc test was done for analysis of cytotoxicity, morphology and migration data. Results: Metformin was lethal to half (LC50) of the cells at 30 mM, and a decrease in viability (P<0.05) was noted at 5 mM. LECs in PCOM treated with 1 mM metformin showed increased Pax6 and E-cadherin and decreased α-SMA and fibronectin expression. LECs in PCOM treated with metformin also maintained epithelial morphology. Migration was inhibited with 0.5 mM metformin (P<0.05). Both HLE-B3 and the lens epithelium in donor eyes were found to express SLC22A1. Conclusion: Metformin decreased survival and migration in LECs, maintaining epithelial phenotype and reducing mesenchymal marker expression. Metformin therefore has potential as an adjunct in PCO prevention.http://www.thepajo.org/article.asp?issn=2666-4909;year=2018;volume=17;issue=4;spage=105;epage=112;aulast=Lasiste;type=0metformin; lens epithelial cells; posterior capsule opacification; epithelial-to-mesenchymal transition. |
| spellingShingle | Jade Marie Lasiste Pablo Zoroquiain Denise Miyamoto Miguel N Burnier Metformin activity in an in vitro model of posterior capsule opacification The Pan-American Journal of Ophthalmology metformin; lens epithelial cells; posterior capsule opacification; epithelial-to-mesenchymal transition. |
| title | Metformin activity in an in vitro model of posterior capsule opacification |
| title_full | Metformin activity in an in vitro model of posterior capsule opacification |
| title_fullStr | Metformin activity in an in vitro model of posterior capsule opacification |
| title_full_unstemmed | Metformin activity in an in vitro model of posterior capsule opacification |
| title_short | Metformin activity in an in vitro model of posterior capsule opacification |
| title_sort | metformin activity in an in vitro model of posterior capsule opacification |
| topic | metformin; lens epithelial cells; posterior capsule opacification; epithelial-to-mesenchymal transition. |
| url | http://www.thepajo.org/article.asp?issn=2666-4909;year=2018;volume=17;issue=4;spage=105;epage=112;aulast=Lasiste;type=0 |
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