Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution
Label-free detection of single-nucleotides was performed by fast tunneling current measurements in a polar solvent at 1 MHz sampling rate using SiO2-protected Au nanoprobes. Short current spikes were observed, suggestive of trapping/detrapping of individual nucleotides between the nanoelectrodes. Th...
| Main Authors: | , , , , |
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| Format: | Article |
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MDPI AG
2017-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/17/4/885 |
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| author | Takanori Morikawa Kazumichi Yokota Sachie Tanimoto Makusu Tsutsui Masateru Taniguchi |
| author_facet | Takanori Morikawa Kazumichi Yokota Sachie Tanimoto Makusu Tsutsui Masateru Taniguchi |
| author_sort | Takanori Morikawa |
| collection | DOAJ |
| description | Label-free detection of single-nucleotides was performed by fast tunneling current measurements in a polar solvent at 1 MHz sampling rate using SiO2-protected Au nanoprobes. Short current spikes were observed, suggestive of trapping/detrapping of individual nucleotides between the nanoelectrodes. The fall and rise features of the electrical signatures indicated signal retardation by capacitance effects with a time constant of about 10 microseconds. The high temporal resolution revealed current fluctuations, reflecting the molecular conformation degrees of freedom in the electrode gap. The method presented in this work may enable direct characterizations of dynamic changes in single-molecule conformations in an electrode gap in liquid. |
| first_indexed | 2024-04-14T02:30:33Z |
| format | Article |
| id | doaj.art-f6e5967259d447bcb4fb93b46894bbf8 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T02:30:33Z |
| publishDate | 2017-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-f6e5967259d447bcb4fb93b46894bbf82022-12-22T02:17:43ZengMDPI AGSensors1424-82202017-04-0117488510.3390/s17040885s17040885Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal ResolutionTakanori Morikawa0Kazumichi Yokota1Sachie Tanimoto2Makusu Tsutsui3Masateru Taniguchi4The Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, JapanThe Institute of Scientific and Industrial Research, Osaka University, 8‐1 Mihogaoka, Ibaraki 567-0047, JapanLabel-free detection of single-nucleotides was performed by fast tunneling current measurements in a polar solvent at 1 MHz sampling rate using SiO2-protected Au nanoprobes. Short current spikes were observed, suggestive of trapping/detrapping of individual nucleotides between the nanoelectrodes. The fall and rise features of the electrical signatures indicated signal retardation by capacitance effects with a time constant of about 10 microseconds. The high temporal resolution revealed current fluctuations, reflecting the molecular conformation degrees of freedom in the electrode gap. The method presented in this work may enable direct characterizations of dynamic changes in single-molecule conformations in an electrode gap in liquid.http://www.mdpi.com/1424-8220/17/4/885tunneling currentsingle moleculesequencing |
| spellingShingle | Takanori Morikawa Kazumichi Yokota Sachie Tanimoto Makusu Tsutsui Masateru Taniguchi Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution Sensors tunneling current single molecule sequencing |
| title | Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution |
| title_full | Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution |
| title_fullStr | Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution |
| title_full_unstemmed | Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution |
| title_short | Detecting Single-Nucleotides by Tunneling Current Measurements at Sub-MHz Temporal Resolution |
| title_sort | detecting single nucleotides by tunneling current measurements at sub mhz temporal resolution |
| topic | tunneling current single molecule sequencing |
| url | http://www.mdpi.com/1424-8220/17/4/885 |
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