Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer
Loss of mitotic regulation is commonly observed in cancer and is a major cause of whole-chromosome aneuploidy. The identification of genes that play a role in the proper progression of mitosis can help us to understand the development and evolution of this disease. Here, we generated a list of prote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/15/3759 |
| _version_ | 1797442608264380416 |
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| author | Alexsandro dos Santos Geneviève Ouellete Caroline Diorio Sabine Elowe Francine Durocher |
| author_facet | Alexsandro dos Santos Geneviève Ouellete Caroline Diorio Sabine Elowe Francine Durocher |
| author_sort | Alexsandro dos Santos |
| collection | DOAJ |
| description | Loss of mitotic regulation is commonly observed in cancer and is a major cause of whole-chromosome aneuploidy. The identification of genes that play a role in the proper progression of mitosis can help us to understand the development and evolution of this disease. Here, we generated a list of proteins implicated in mitosis that we used to probe a patient-derived breast cancer (BC) continuum gene-expression dataset generated by our group by human transcriptome analysis of breast lesions of varying aggressiveness (from normal to invasive). We identified cytoskeleton-associated protein 2 (CKAP2) as an important mitotic regulator in invasive BC. The results showed that CKAP2 is overexpressed in invasive BC tumors when compared with normal tissues, and highly expressed in all BC subtypes. Higher expression of CKAP2 is also related to a worse prognosis in overall survival and relapse-free survival in estrogen receptor (ER)-positive and human epidermal growth factor receptor type 2 (HER2)-negative BC patients. Knockdown of CKAP2 in SKBR3 cells impaired cell proliferation and cell migration and reduced aggregate formation in a 3D culture. Our results show the important role of CKAP2 in BC tumorigenesis, and its potential utility as a prognostic marker in BC. |
| first_indexed | 2024-03-09T12:44:25Z |
| format | Article |
| id | doaj.art-f6e7b2465f654511bf8f41e3323d8022 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T12:44:25Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f6e7b2465f654511bf8f41e3323d80222023-11-30T22:14:41ZengMDPI AGCancers2072-66942022-08-011415375910.3390/cancers14153759Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast CancerAlexsandro dos Santos0Geneviève Ouellete1Caroline Diorio2Sabine Elowe3Francine Durocher4Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, Québec City, QC G1V 0A6, CanadaDépartement de Médecine Moléculaire, Faculté de Médecine, Université Laval, Québec City, QC G1V 0A6, CanadaCentre de Recherche sur le Cancer, CHU de Québec-Université Laval, Québec City, QC G1V 4G2, CanadaCentre de Recherche sur le Cancer, CHU de Québec-Université Laval, Québec City, QC G1V 4G2, CanadaDépartement de Médecine Moléculaire, Faculté de Médecine, Université Laval, Québec City, QC G1V 0A6, CanadaLoss of mitotic regulation is commonly observed in cancer and is a major cause of whole-chromosome aneuploidy. The identification of genes that play a role in the proper progression of mitosis can help us to understand the development and evolution of this disease. Here, we generated a list of proteins implicated in mitosis that we used to probe a patient-derived breast cancer (BC) continuum gene-expression dataset generated by our group by human transcriptome analysis of breast lesions of varying aggressiveness (from normal to invasive). We identified cytoskeleton-associated protein 2 (CKAP2) as an important mitotic regulator in invasive BC. The results showed that CKAP2 is overexpressed in invasive BC tumors when compared with normal tissues, and highly expressed in all BC subtypes. Higher expression of CKAP2 is also related to a worse prognosis in overall survival and relapse-free survival in estrogen receptor (ER)-positive and human epidermal growth factor receptor type 2 (HER2)-negative BC patients. Knockdown of CKAP2 in SKBR3 cells impaired cell proliferation and cell migration and reduced aggregate formation in a 3D culture. Our results show the important role of CKAP2 in BC tumorigenesis, and its potential utility as a prognostic marker in BC.https://www.mdpi.com/2072-6694/14/15/3759breast cancermitosisCKAP2tumorigenesisprognostic biomarker |
| spellingShingle | Alexsandro dos Santos Geneviève Ouellete Caroline Diorio Sabine Elowe Francine Durocher Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer Cancers breast cancer mitosis CKAP2 tumorigenesis prognostic biomarker |
| title | Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer |
| title_full | Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer |
| title_fullStr | Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer |
| title_full_unstemmed | Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer |
| title_short | Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer |
| title_sort | knockdown of ckap2 inhibits proliferation migration and aggregate formation in aggressive breast cancer |
| topic | breast cancer mitosis CKAP2 tumorigenesis prognostic biomarker |
| url | https://www.mdpi.com/2072-6694/14/15/3759 |
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