A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales
ABSTRACT The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology. We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria. Using Rhodob...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mbio.01203-23 |
| _version_ | 1797730293417771008 |
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| author | François Alberge Bryan D. Lakey Ryan E. Schaub Alice C. Dohnalkova Kimberley C. Lemmer Joseph P. Dillard Daniel R. Noguera Timothy J. Donohue |
| author_facet | François Alberge Bryan D. Lakey Ryan E. Schaub Alice C. Dohnalkova Kimberley C. Lemmer Joseph P. Dillard Daniel R. Noguera Timothy J. Donohue |
| author_sort | François Alberge |
| collection | DOAJ |
| description | ABSTRACT The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology. We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria. Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG). Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division. We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides’ divisome. Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases. We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division. Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division. We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria. Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria. IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds. The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation. However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny. We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function. We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule. The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria. |
| first_indexed | 2024-03-12T11:42:10Z |
| format | Article |
| id | doaj.art-f6e9f1e1e39347b8b0284fcd7a699153 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-03-12T11:42:10Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-f6e9f1e1e39347b8b0284fcd7a6991532023-08-31T15:04:20ZengAmerican Society for MicrobiologymBio2150-75112023-08-0114410.1128/mbio.01203-23A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in RhodobacteralesFrançois Alberge0Bryan D. Lakey1Ryan E. Schaub2Alice C. Dohnalkova3Kimberley C. Lemmer4Joseph P. Dillard5Daniel R. Noguera6Timothy J. Donohue71 Wisconsin Energy Institute, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison , Madison, Wisconsin, USA1 Wisconsin Energy Institute, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison , Madison, Wisconsin, USADepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USAEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , Richland, Washington, USANational Renewable Energy Laboratory , Golden, Colorado, USADepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA1 Wisconsin Energy Institute, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison , Madison, Wisconsin, USA1 Wisconsin Energy Institute, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison , Madison, Wisconsin, USAABSTRACT The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology. We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria. Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG). Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division. We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides’ divisome. Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases. We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division. Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division. We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria. Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria. IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds. The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation. However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny. We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function. We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule. The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.https://journals.asm.org/doi/10.1128/mbio.01203-23cell divisionAlphaproteobacteriapeptidoglycancell envelopelipoprotein |
| spellingShingle | François Alberge Bryan D. Lakey Ryan E. Schaub Alice C. Dohnalkova Kimberley C. Lemmer Joseph P. Dillard Daniel R. Noguera Timothy J. Donohue A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales mBio cell division Alphaproteobacteria peptidoglycan cell envelope lipoprotein |
| title | A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales |
| title_full | A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales |
| title_fullStr | A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales |
| title_full_unstemmed | A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales |
| title_short | A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales |
| title_sort | previously uncharacterized divisome associated lipoprotein dala is needed for normal cell division in rhodobacterales |
| topic | cell division Alphaproteobacteria peptidoglycan cell envelope lipoprotein |
| url | https://journals.asm.org/doi/10.1128/mbio.01203-23 |
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