Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.)
Mung bean is vulnerable to bruchids (<i>Callosobruchus</i> spp.), resulting in low quality and losses worldwide. Developing resistant cultivars is the most effective, economical, and eco-friendly way to protect mung bean seeds from the damage of bruchids. Previously, we identified two ge...
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/6/1271 |
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| author | Ranran Wu Qinxue Zhang Yun Lin Jingbin Chen Prakit Somta Qiang Yan Chenchen Xue Jinyang Liu Xin Chen Xingxing Yuan |
| author_facet | Ranran Wu Qinxue Zhang Yun Lin Jingbin Chen Prakit Somta Qiang Yan Chenchen Xue Jinyang Liu Xin Chen Xingxing Yuan |
| author_sort | Ranran Wu |
| collection | DOAJ |
| description | Mung bean is vulnerable to bruchids (<i>Callosobruchus</i> spp.), resulting in low quality and losses worldwide. Developing resistant cultivars is the most effective, economical, and eco-friendly way to protect mung bean seeds from the damage of bruchids. Previously, we identified two gene loci that are tightly linked with bruchid resistance, which makes new cultivar development possible using molecular assisted selection (MAS). In the present study, marker-assisted backcross (MABC) breeding was employed to introgress the bruchid resistance gene <i>VrPGIP2</i> locus from the donor parent V2802 into a popular cultivar, Kamphaeng Saen 1. Markers VrBR-SSR013 and DMB-SSR158 were used for foreground selection, and phenotypic selection was used for background selection. Three advanced inbred lines (R67-11, R67-22, and R92-15) carrying the resistance gene <i>VrPGIP2</i> were developed from the BC<sub>3</sub>F<sub>6</sub> population. Further analysis showed that the line R67-22 performed best; it was highly resistant to bruchids and showed excellent agronomic traits in the field. Therefore, R67-22 could be further evaluated in large-scale trials for release as a variety. |
| first_indexed | 2024-03-10T00:40:48Z |
| format | Article |
| id | doaj.art-f6eacbad33d04656a603db93ee825544 |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:40:48Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-f6eacbad33d04656a603db93ee8255442023-11-23T15:09:03ZengMDPI AGAgronomy2073-43952022-05-01126127110.3390/agronomy12061271Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.)Ranran Wu0Qinxue Zhang1Yun Lin2Jingbin Chen3Prakit Somta4Qiang Yan5Chenchen Xue6Jinyang Liu7Xin Chen8Xingxing Yuan9Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaSchool of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaDepartment of Agronomy, Faculty of Agriculture at Kampaheng Saen, Kasetsart University, Nakhon Pathom 73140, ThailandInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaMung bean is vulnerable to bruchids (<i>Callosobruchus</i> spp.), resulting in low quality and losses worldwide. Developing resistant cultivars is the most effective, economical, and eco-friendly way to protect mung bean seeds from the damage of bruchids. Previously, we identified two gene loci that are tightly linked with bruchid resistance, which makes new cultivar development possible using molecular assisted selection (MAS). In the present study, marker-assisted backcross (MABC) breeding was employed to introgress the bruchid resistance gene <i>VrPGIP2</i> locus from the donor parent V2802 into a popular cultivar, Kamphaeng Saen 1. Markers VrBR-SSR013 and DMB-SSR158 were used for foreground selection, and phenotypic selection was used for background selection. Three advanced inbred lines (R67-11, R67-22, and R92-15) carrying the resistance gene <i>VrPGIP2</i> were developed from the BC<sub>3</sub>F<sub>6</sub> population. Further analysis showed that the line R67-22 performed best; it was highly resistant to bruchids and showed excellent agronomic traits in the field. Therefore, R67-22 could be further evaluated in large-scale trials for release as a variety.https://www.mdpi.com/2073-4395/12/6/1271mung beanbruchid resistance<i>VrPGIP</i>marker-assisted backcrossing (MABC)host-plant resistance breeding |
| spellingShingle | Ranran Wu Qinxue Zhang Yun Lin Jingbin Chen Prakit Somta Qiang Yan Chenchen Xue Jinyang Liu Xin Chen Xingxing Yuan Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) Agronomy mung bean bruchid resistance <i>VrPGIP</i> marker-assisted backcrossing (MABC) host-plant resistance breeding |
| title | Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) |
| title_full | Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) |
| title_fullStr | Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) |
| title_full_unstemmed | Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) |
| title_short | Marker-Assisted Backcross Breeding for Improving Bruchid (<i>Callosobruchus</i> spp.) Resistance in Mung Bean (<i>Vigna radiata</i> L.) |
| title_sort | marker assisted backcross breeding for improving bruchid i callosobruchus i spp resistance in mung bean i vigna radiata i l |
| topic | mung bean bruchid resistance <i>VrPGIP</i> marker-assisted backcrossing (MABC) host-plant resistance breeding |
| url | https://www.mdpi.com/2073-4395/12/6/1271 |
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