Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
ABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2018-02-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00017-18 |
| _version_ | 1831548208163061760 |
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| author | Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan |
| author_facet | Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan |
| author_sort | Mi Zhou |
| collection | DOAJ |
| description | ABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels (0, 1, 3, and 5%) on rumen microbiota and rumen/fecal E. coli O serogroups in rams were investigated. Rumen total bacteria and archaea were linearly reduced (P < 0.001) and protozoa were linearly increased (P < 0.001) by increasing levels of Tasco. The relative abundances of seven bacterial species and one protozoal species differed among Tasco levels. With Tasco, 14 predicted metabolic pathways were enriched while only 3 were suppressed. A lower ruminal butyrate concentration is possibly associated with enrichment of the “butanoate metabolism” pathway in Tasco-fed rams. The ruminal total E. coli population was linearly reduced (P < 0.001) by Tasco. Supplementation with Tasco only completely eliminated O121 in the rumen and feces, and higher levels of Tasco (3 and 5%) reduced fecal shedding of serogroups O45, O103, and O111 even though these serogroups were present in the rumen. Our results suggest that Tasco effectively reduced pathogenic E. coli but had only minimal impacts on rumen fermentation in rams. IMPORTANCE Maintaining product safety and reducing the carbon footprint of production are two sustainability goals of the livestock industry. The objective of this study was to study the impact of Tasco, a product derived from the brown macroalga Ascophyllum nodosum, on the rumen microbiome and its function. The inclusion of Tasco altered both rumen and fecal microbiota levels without affecting rumen fermentation. Tasco reduced fecal Escherichia coli populations and specifically reduced the prevalence of Shiga toxin-producing E. coli O45, O103, O111, and O121 in feces. The findings of this study highlight the application of Tasco as a potential feed additive to reduce pathogen shedding in rams without interfering with ruminal metabolism. |
| first_indexed | 2024-12-17T02:14:59Z |
| format | Article |
| id | doaj.art-f6ec53f3afed450b8053935ab3ef8543 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-17T02:14:59Z |
| publishDate | 2018-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-f6ec53f3afed450b8053935ab3ef85432022-12-21T22:07:26ZengAmerican Society for MicrobiologymSphere2379-50422018-02-013110.1128/mSphere.00017-18Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne PathogensMi Zhou0Martin Hünerberg1Yanhong Chen2Tim Reuter3Tim A. McAllister4Franklin Evans5Alan T. Critchley6Le Luo Guan7Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaLethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaAlberta Agriculture and Rural Development, Agriculture Centre, Lethbridge, Alberta, CanadaLethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, CanadaAcadian Seaplants Ltd., Dartmouth, Nova Scotia, CanadaAcadian Seaplants Ltd., Dartmouth, Nova Scotia, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels (0, 1, 3, and 5%) on rumen microbiota and rumen/fecal E. coli O serogroups in rams were investigated. Rumen total bacteria and archaea were linearly reduced (P < 0.001) and protozoa were linearly increased (P < 0.001) by increasing levels of Tasco. The relative abundances of seven bacterial species and one protozoal species differed among Tasco levels. With Tasco, 14 predicted metabolic pathways were enriched while only 3 were suppressed. A lower ruminal butyrate concentration is possibly associated with enrichment of the “butanoate metabolism” pathway in Tasco-fed rams. The ruminal total E. coli population was linearly reduced (P < 0.001) by Tasco. Supplementation with Tasco only completely eliminated O121 in the rumen and feces, and higher levels of Tasco (3 and 5%) reduced fecal shedding of serogroups O45, O103, and O111 even though these serogroups were present in the rumen. Our results suggest that Tasco effectively reduced pathogenic E. coli but had only minimal impacts on rumen fermentation in rams. IMPORTANCE Maintaining product safety and reducing the carbon footprint of production are two sustainability goals of the livestock industry. The objective of this study was to study the impact of Tasco, a product derived from the brown macroalga Ascophyllum nodosum, on the rumen microbiome and its function. The inclusion of Tasco altered both rumen and fecal microbiota levels without affecting rumen fermentation. Tasco reduced fecal Escherichia coli populations and specifically reduced the prevalence of Shiga toxin-producing E. coli O45, O103, O111, and O121 in feces. The findings of this study highlight the application of Tasco as a potential feed additive to reduce pathogen shedding in rams without interfering with ruminal metabolism.https://journals.asm.org/doi/10.1128/mSphere.00017-18Ascophyllum nodosumEscherichia coliTascoramrumen microbiomeseaweed |
| spellingShingle | Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens mSphere Ascophyllum nodosum Escherichia coli Tasco ram rumen microbiome seaweed |
| title | Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens |
| title_full | Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens |
| title_fullStr | Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens |
| title_full_unstemmed | Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens |
| title_short | Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens |
| title_sort | air dried brown seaweed italic toggle yes ascophyllum nodosum italic alters the rumen microbiome in a manner that changes rumen fermentation profiles and lowers the prevalence of foodborne pathogens |
| topic | Ascophyllum nodosum Escherichia coli Tasco ram rumen microbiome seaweed |
| url | https://journals.asm.org/doi/10.1128/mSphere.00017-18 |
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