<i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract
Cigarette smoke (CS) is the major factor in the development of chronic obstructive pulmonary disease (COPD), the third leading cause of death worldwide. Furthermore, although <i>Camellia sinensis</i> (CN) has been known as an anti-inflammatory material, the effect of CN has not yet been...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/9/1683 |
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| author | Dong-Uk Shin Ji-Eun Eom Hyeon-Ji Song Sun Young Jung Thi Van Nguyen Kyung Min Lim Ok Hee Chai Hyun-Jin Kim Gun-Dong Kim Hee Soon Shin So-Young Lee |
| author_facet | Dong-Uk Shin Ji-Eun Eom Hyeon-Ji Song Sun Young Jung Thi Van Nguyen Kyung Min Lim Ok Hee Chai Hyun-Jin Kim Gun-Dong Kim Hee Soon Shin So-Young Lee |
| author_sort | Dong-Uk Shin |
| collection | DOAJ |
| description | Cigarette smoke (CS) is the major factor in the development of chronic obstructive pulmonary disease (COPD), the third leading cause of death worldwide. Furthermore, although <i>Camellia sinensis</i> (CN) has been known as an anti-inflammatory material, the effect of CN has not yet been known on pulmonary inflammation in COPD. Thus, we investigated the protective effects of <i>Camellia sinensis</i> L. extract (CLE) against pulmonary inflammation in porcine pancreas elastase (PPE) and a cigarette smoke extract (CSE)-induced COPD mouse model. Oral administration of CLE suppressed the symptoms such as infiltration of immune cells, cytokines/chemokines secretion, mucus hypersecretion, and injuries of the lung parenchyma. Increased inflammatory responses in COPD are mediated by various immune cells such as airway epithelial cells, neutrophils, and alveolar macrophages. Thus, we investigated the effect and mechanisms of CLE in H292, HL-60, and MH-S cells. The CLE inhibited the expression of IL-6, IL-8, MUC5AC and MUC5B on CSE/LPS-stimulated H292 cells and also suppressed the formation of neutrophil extracellular traps and secretion of neutrophil elastase by inhibiting reactive oxygen species in PMA-induced HL-60 cells. In particular, the CLE suppressed the release of cytokines and chemokines caused by activating the nuclear factor kappa-light-chain-enhancer of activated B via the activation of nuclear factor erythroid-2-related factor 2 and the heme oxygenase-1 pathway in CSE/LPS-stimulated MH-S cells. Therefore, we suggest that the CLE administration be the effective approach for treating or preventing chronic pulmonary diseases such as COPD induced by CS. |
| first_indexed | 2024-03-10T00:54:22Z |
| format | Article |
| id | doaj.art-f6ef4bd86d164a8d92d30f7e6e273209 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:54:22Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f6ef4bd86d164a8d92d30f7e6e2732092023-11-23T14:47:14ZengMDPI AGAntioxidants2076-39212022-08-01119168310.3390/antiox11091683<i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke ExtractDong-Uk Shin0Ji-Eun Eom1Hyeon-Ji Song2Sun Young Jung3Thi Van Nguyen4Kyung Min Lim5Ok Hee Chai6Hyun-Jin Kim7Gun-Dong Kim8Hee Soon Shin9So-Young Lee10Department of Food Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, KoreaFood Function Infrastructure Team, Korea Food Research Institute (KFRI), Wanju 55365, KoreaDivision of Food Functionality Research, Korea Food Research Institute (KFRI), Wanju 55365, KoreaDepartment of Food Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, KoreaDepartment of Anatomy, Institute of Medical Science, Jeonbuk National University Medical School, Jeonju 54907, KoreaDepartment of Food Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, KoreaDepartment of Anatomy, Institute of Medical Science, Jeonbuk National University Medical School, Jeonju 54907, KoreaDivision of Applied Life Science (BK21 Four), Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinjudaero, Jinju 52828, KoreaDivision of Food Functionality Research, Korea Food Research Institute (KFRI), Wanju 55365, KoreaDepartment of Food Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, KoreaDepartment of Food Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, KoreaCigarette smoke (CS) is the major factor in the development of chronic obstructive pulmonary disease (COPD), the third leading cause of death worldwide. Furthermore, although <i>Camellia sinensis</i> (CN) has been known as an anti-inflammatory material, the effect of CN has not yet been known on pulmonary inflammation in COPD. Thus, we investigated the protective effects of <i>Camellia sinensis</i> L. extract (CLE) against pulmonary inflammation in porcine pancreas elastase (PPE) and a cigarette smoke extract (CSE)-induced COPD mouse model. Oral administration of CLE suppressed the symptoms such as infiltration of immune cells, cytokines/chemokines secretion, mucus hypersecretion, and injuries of the lung parenchyma. Increased inflammatory responses in COPD are mediated by various immune cells such as airway epithelial cells, neutrophils, and alveolar macrophages. Thus, we investigated the effect and mechanisms of CLE in H292, HL-60, and MH-S cells. The CLE inhibited the expression of IL-6, IL-8, MUC5AC and MUC5B on CSE/LPS-stimulated H292 cells and also suppressed the formation of neutrophil extracellular traps and secretion of neutrophil elastase by inhibiting reactive oxygen species in PMA-induced HL-60 cells. In particular, the CLE suppressed the release of cytokines and chemokines caused by activating the nuclear factor kappa-light-chain-enhancer of activated B via the activation of nuclear factor erythroid-2-related factor 2 and the heme oxygenase-1 pathway in CSE/LPS-stimulated MH-S cells. Therefore, we suggest that the CLE administration be the effective approach for treating or preventing chronic pulmonary diseases such as COPD induced by CS.https://www.mdpi.com/2076-3921/11/9/1683cigarette smokechronic obstructive pulmonary diseaseslung inflammationairway epithelialalveolar macrophagesneutrophils |
| spellingShingle | Dong-Uk Shin Ji-Eun Eom Hyeon-Ji Song Sun Young Jung Thi Van Nguyen Kyung Min Lim Ok Hee Chai Hyun-Jin Kim Gun-Dong Kim Hee Soon Shin So-Young Lee <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract Antioxidants cigarette smoke chronic obstructive pulmonary diseases lung inflammation airway epithelial alveolar macrophages neutrophils |
| title | <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract |
| title_full | <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract |
| title_fullStr | <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract |
| title_full_unstemmed | <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract |
| title_short | <i>Camellia sinensis</i> L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract |
| title_sort | i camellia sinensis i l alleviates pulmonary inflammation induced by porcine pancreas elastase and cigarette smoke extract |
| topic | cigarette smoke chronic obstructive pulmonary diseases lung inflammation airway epithelial alveolar macrophages neutrophils |
| url | https://www.mdpi.com/2076-3921/11/9/1683 |
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