Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs
Abstract Background Mesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for a variety of conditions. So far, the precise mode of action of the MSCs has yet to be determined. The aim of this study was to gain more ins...
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-021-02457-9 |
| _version_ | 1819154831957295104 |
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| author | Charlotte Beerts Carlien Brondeel Glenn Pauwelyn Eva Depuydt Liesa Tack Luc Duchateau Yangfeng Xu Jimmy H. Saunders Kathelijne Peremans Jan H. Spaas |
| author_facet | Charlotte Beerts Carlien Brondeel Glenn Pauwelyn Eva Depuydt Liesa Tack Luc Duchateau Yangfeng Xu Jimmy H. Saunders Kathelijne Peremans Jan H. Spaas |
| author_sort | Charlotte Beerts |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for a variety of conditions. So far, the precise mode of action of the MSCs has yet to be determined. The aim of this study was to gain more insights into the pharmacokinetics of MSCs by evaluating their biodistribution in healthy dogs after different injection routes. Methods Three different studies were performed in healthy dogs to evaluate the biodistribution pattern of radiolabelled equine peripheral blood-derived mesenchymal stem cells following intravenous, intramuscular and subcutaneous administration in comparison with free 99mTechnetium. The labelling of the equine peripheral blood-derived mesenchymal stem cells was performed using stannous chloride as a reducing agent. Whole-body scans were obtained using a gamma camera during a 24-h follow-up. Results The labelling efficiency ranged between 59.58 and 83.82%. Free 99mTechnetium accumulation was predominantly observed in the stomach, thyroid, bladder and salivary glands, while following intravenous injection, the 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells majorly accumulated in the liver throughout the follow-up period. After intramuscular and subcutaneous injection, the injected dose percentage remained very high at the injection site. Conclusions A distinct difference was noted in the biodistribution pattern of the radiolabelled equine peripheral blood-derived mesenchymal stem cells compared to free 99mTechnetium indicating equine peripheral blood-derived mesenchymal stem cells have a specific pharmacokinetic pattern after systemic administration in healthy dogs. Furthermore, the biodistribution pattern of the used xenogeneic equine peripheral blood-derived mesenchymal stem cells appeared to be different from previously reported experiments using different sources of mesenchymal stem cells. |
| first_indexed | 2024-12-22T15:27:20Z |
| format | Article |
| id | doaj.art-f6ef7d77df224e1a8cb4e5575acbef51 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-22T15:27:20Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-f6ef7d77df224e1a8cb4e5575acbef512022-12-21T18:21:27ZengBMCStem Cell Research & Therapy1757-65122021-07-0112111110.1186/s13287-021-02457-9Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogsCharlotte Beerts0Carlien Brondeel1Glenn Pauwelyn2Eva Depuydt3Liesa Tack4Luc Duchateau5Yangfeng Xu6Jimmy H. Saunders7Kathelijne Peremans8Jan H. Spaas9Global Stem cell Technology NVDepartment of Medical Imaging and Orthopedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent UniversityGlobal Stem cell Technology NVGlobal Stem cell Technology NVGlobal Stem cell Technology NVBiometrics Research Center, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Medical Imaging and Orthopedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Medical Imaging and Orthopedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Medical Imaging and Orthopedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent UniversityGlobal Stem cell Technology NVAbstract Background Mesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for a variety of conditions. So far, the precise mode of action of the MSCs has yet to be determined. The aim of this study was to gain more insights into the pharmacokinetics of MSCs by evaluating their biodistribution in healthy dogs after different injection routes. Methods Three different studies were performed in healthy dogs to evaluate the biodistribution pattern of radiolabelled equine peripheral blood-derived mesenchymal stem cells following intravenous, intramuscular and subcutaneous administration in comparison with free 99mTechnetium. The labelling of the equine peripheral blood-derived mesenchymal stem cells was performed using stannous chloride as a reducing agent. Whole-body scans were obtained using a gamma camera during a 24-h follow-up. Results The labelling efficiency ranged between 59.58 and 83.82%. Free 99mTechnetium accumulation was predominantly observed in the stomach, thyroid, bladder and salivary glands, while following intravenous injection, the 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells majorly accumulated in the liver throughout the follow-up period. After intramuscular and subcutaneous injection, the injected dose percentage remained very high at the injection site. Conclusions A distinct difference was noted in the biodistribution pattern of the radiolabelled equine peripheral blood-derived mesenchymal stem cells compared to free 99mTechnetium indicating equine peripheral blood-derived mesenchymal stem cells have a specific pharmacokinetic pattern after systemic administration in healthy dogs. Furthermore, the biodistribution pattern of the used xenogeneic equine peripheral blood-derived mesenchymal stem cells appeared to be different from previously reported experiments using different sources of mesenchymal stem cells.https://doi.org/10.1186/s13287-021-02457-9Mesenchymal stem cellsXenogeneicEquine peripheral bloodScintigraphyBiodistributionCanine |
| spellingShingle | Charlotte Beerts Carlien Brondeel Glenn Pauwelyn Eva Depuydt Liesa Tack Luc Duchateau Yangfeng Xu Jimmy H. Saunders Kathelijne Peremans Jan H. Spaas Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs Stem Cell Research & Therapy Mesenchymal stem cells Xenogeneic Equine peripheral blood Scintigraphy Biodistribution Canine |
| title | Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs |
| title_full | Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs |
| title_fullStr | Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs |
| title_full_unstemmed | Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs |
| title_short | Scintigraphic tracking of 99mTechnetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs |
| title_sort | scintigraphic tracking of 99mtechnetium labelled equine peripheral blood derived mesenchymal stem cells after intravenous intramuscular and subcutaneous injection in healthy dogs |
| topic | Mesenchymal stem cells Xenogeneic Equine peripheral blood Scintigraphy Biodistribution Canine |
| url | https://doi.org/10.1186/s13287-021-02457-9 |
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