The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity?
The western Palaearctic species of the hawkmoth genus <i>Hyles</i> (Lepidoptera: Sphingidae) have long been the subject of molecular phylogenetic research. However, much less attention has been paid to the taxa inhabiting the central and eastern Palaearctic, particularly Central Asia, wh...
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MDPI AG
2021-05-01
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| author | Franziska Patzold Eduardo Marabuto Hana Daneck Mark A. O’Neill Ian J. Kitching Anna K. Hundsdoerfer |
| author_facet | Franziska Patzold Eduardo Marabuto Hana Daneck Mark A. O’Neill Ian J. Kitching Anna K. Hundsdoerfer |
| author_sort | Franziska Patzold |
| collection | DOAJ |
| description | The western Palaearctic species of the hawkmoth genus <i>Hyles</i> (Lepidoptera: Sphingidae) have long been the subject of molecular phylogenetic research. However, much less attention has been paid to the taxa inhabiting the central and eastern Palaearctic, particularly Central Asia, where almost 50% of the species diversity of the genus occurs. Yet, many taxonomic conundrums hinder a proper assessment of the true diversity in these moths. One still unresolved group of species includes <i>Hyles hippophaes</i> and <i>Hyles chamyla</i>. Despite a largely overlapping morphology and ecology, a plethora of infraspecific taxa display some unique divergent characters over a wide geographical area. In this study, we undertook a taxonomic assessment of each population and resolved this species complex using an integrative approach. A combination of new computational techniques (DAISY-II) in comparative morphology and recent advances in DNA extraction methods and sequencing of museum specimens (WISC) alongside more traditional genetic approaches allowed testing of the three main phenotypes—bienerti, chamyla and apocyni—in terms of their morphological, mitochondrial and biogeographical integrity, and to elucidate their evolutionary relationships. Our results support the existence of two closely related species, <i>Hyles chamyla</i> and <i>H. hippophaes</i>, but the former species <i>H. apocyni</i> (here discussed as the ecological form apocyni of <i>H. chamyla</i>) is best regarded as a hybrid between <i>H. chamyla</i> and <i>H. h. bienerti</i>. The results indicate that the evolutionary relationship between <i>H. chamyla</i> and <i>H. hippophaes</i> is one of admixture in the context of ongoing ecological differentiation, which has led to shared morphological characters and a blurring of the species boundaries. These results clarify the evolutionary relationships of this species complex and open future research lines, including the analysis of nuclear markers and denser sampling, particularly of <i>H. hippophaes</i> and <i>H. vespertilio</i> in western Europe. |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T11:19:35Z |
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| spelling | doaj.art-f6ef988e0f964dc18b6a17ba067ca0ba2023-11-21T20:09:25ZengMDPI AGDiversity1424-28182021-05-0113521310.3390/d13050213The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity?Franziska Patzold0Eduardo Marabuto1Hana Daneck2Mark A. O’Neill3Ian J. Kitching4Anna K. Hundsdoerfer5Museum of Zoology, Senckenberg Natural History Collections Dresden, Königsbrücker Landstr. 159, D-01109 Dresden, GermanyMuseum of Zoology, Senckenberg Natural History Collections Dresden, Königsbrücker Landstr. 159, D-01109 Dresden, GermanyMuseum of Zoology, Senckenberg Natural History Collections Dresden, Königsbrücker Landstr. 159, D-01109 Dresden, GermanyTumbling Dice, Newcastle Upon Tyne NE3 4RT, UKDepartment of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, UKMuseum of Zoology, Senckenberg Natural History Collections Dresden, Königsbrücker Landstr. 159, D-01109 Dresden, GermanyThe western Palaearctic species of the hawkmoth genus <i>Hyles</i> (Lepidoptera: Sphingidae) have long been the subject of molecular phylogenetic research. However, much less attention has been paid to the taxa inhabiting the central and eastern Palaearctic, particularly Central Asia, where almost 50% of the species diversity of the genus occurs. Yet, many taxonomic conundrums hinder a proper assessment of the true diversity in these moths. One still unresolved group of species includes <i>Hyles hippophaes</i> and <i>Hyles chamyla</i>. Despite a largely overlapping morphology and ecology, a plethora of infraspecific taxa display some unique divergent characters over a wide geographical area. In this study, we undertook a taxonomic assessment of each population and resolved this species complex using an integrative approach. A combination of new computational techniques (DAISY-II) in comparative morphology and recent advances in DNA extraction methods and sequencing of museum specimens (WISC) alongside more traditional genetic approaches allowed testing of the three main phenotypes—bienerti, chamyla and apocyni—in terms of their morphological, mitochondrial and biogeographical integrity, and to elucidate their evolutionary relationships. Our results support the existence of two closely related species, <i>Hyles chamyla</i> and <i>H. hippophaes</i>, but the former species <i>H. apocyni</i> (here discussed as the ecological form apocyni of <i>H. chamyla</i>) is best regarded as a hybrid between <i>H. chamyla</i> and <i>H. h. bienerti</i>. The results indicate that the evolutionary relationship between <i>H. chamyla</i> and <i>H. hippophaes</i> is one of admixture in the context of ongoing ecological differentiation, which has led to shared morphological characters and a blurring of the species boundaries. These results clarify the evolutionary relationships of this species complex and open future research lines, including the analysis of nuclear markers and denser sampling, particularly of <i>H. hippophaes</i> and <i>H. vespertilio</i> in western Europe.https://www.mdpi.com/1424-2818/13/5/213molecular ecologydeep/machine learningmorphological analysis |
| spellingShingle | Franziska Patzold Eduardo Marabuto Hana Daneck Mark A. O’Neill Ian J. Kitching Anna K. Hundsdoerfer The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? Diversity molecular ecology deep/machine learning morphological analysis |
| title | The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? |
| title_full | The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? |
| title_fullStr | The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? |
| title_full_unstemmed | The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? |
| title_short | The Phylogenetics and Biogeography of the Central Asian Hawkmoths, <i>Hyles hippophaes</i> and <i>H. chamyla</i>: Can Mitogenomics and Machine Learning Bring Clarity? |
| title_sort | phylogenetics and biogeography of the central asian hawkmoths i hyles hippophaes i and i h chamyla i can mitogenomics and machine learning bring clarity |
| topic | molecular ecology deep/machine learning morphological analysis |
| url | https://www.mdpi.com/1424-2818/13/5/213 |
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