Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats
This work presents the characteristics of the catalytic activity of hydroxylation of benzo[a] pyrene (B[a]P) in rat liver microsomes using plasma lipoproteins (LP) (VLDL, LDL, HDL) as transport forms of B[a]P. The aim of the study was: to study the effect of the LP-component of the LP-B[a]P complexe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics
2020-12-01
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| Series: | Атеросклероз |
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| Online Access: | https://ateroskleroz.elpub.ru/jour/article/view/394/374 |
| _version_ | 1828076288545390592 |
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| author | L. M. Polyakov R. A. Knyazev N. V. Trifonova M. V. Kotova E. I. Solovyova A. V. Ryabchenko |
| author_facet | L. M. Polyakov R. A. Knyazev N. V. Trifonova M. V. Kotova E. I. Solovyova A. V. Ryabchenko |
| author_sort | L. M. Polyakov |
| collection | DOAJ |
| description | This work presents the characteristics of the catalytic activity of hydroxylation of benzo[a] pyrene (B[a]P) in rat liver microsomes using plasma lipoproteins (LP) (VLDL, LDL, HDL) as transport forms of B[a]P. The aim of the study was: to study the effect of the LP-component of the LP-B[a]P complexes on the rate of B[a]P hydroxylation in rat liver microsomes. The studies were carried out on the microsomal fraction of rat liver using B[a]P as a substrate, ultracentrifugation of individual fractions of VLDL, LDL, HDL plasma, and spectrofluorimetric determination of the activity of arylhydrocarbonhydroxylase. In work on microsomes of rat liver the rate of hydroxylation of B[a]P in free form and in the form of B[a]P complexes with various fractions of LP was analyzed. The analysis showed that the arylhydrocarbonhydroxylase activity (pmol 3-OH-B[a]P in 1 min per 1 mg of protein) decreases in the complex form B[a]P with LP. So for transport form B[a]P with HDL the decrease was by 22%, for transport form B[a]P with VLDL the decrease was 30%, and for transport form B[a]P with LDL it was 52%. It should be noted that Km for the substrate remained practically unchanged in all forms. The Vmax and Km values indicate that the LP component in complexes with B[a]P can be a noncompetitive inhibitor of B[a]P hydroxylation in rat liver microsomes. |
| first_indexed | 2024-04-11T02:12:23Z |
| format | Article |
| id | doaj.art-f6f12d58f58d4af985ac868145b2ba49 |
| institution | Directory Open Access Journal |
| issn | 2078-256X |
| language | Russian |
| last_indexed | 2024-04-11T02:12:23Z |
| publishDate | 2020-12-01 |
| publisher | Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics |
| record_format | Article |
| series | Атеросклероз |
| spelling | doaj.art-f6f12d58f58d4af985ac868145b2ba492023-01-03T02:00:13ZrusSiberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and GeneticsАтеросклероз2078-256X2020-12-011641317https://doi.org/10.15372/ATER20200402Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of ratsL. M. Polyakov0https://orcid.org/0000-0001-5905-8969R. A. Knyazev1N. V. Trifonova2M. V. Kotova3E. I. Solovyova4A. V. Ryabchenko5Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationResearch Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationResearch Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationResearch Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationResearch Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationResearch Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine Russian FederationThis work presents the characteristics of the catalytic activity of hydroxylation of benzo[a] pyrene (B[a]P) in rat liver microsomes using plasma lipoproteins (LP) (VLDL, LDL, HDL) as transport forms of B[a]P. The aim of the study was: to study the effect of the LP-component of the LP-B[a]P complexes on the rate of B[a]P hydroxylation in rat liver microsomes. The studies were carried out on the microsomal fraction of rat liver using B[a]P as a substrate, ultracentrifugation of individual fractions of VLDL, LDL, HDL plasma, and spectrofluorimetric determination of the activity of arylhydrocarbonhydroxylase. In work on microsomes of rat liver the rate of hydroxylation of B[a]P in free form and in the form of B[a]P complexes with various fractions of LP was analyzed. The analysis showed that the arylhydrocarbonhydroxylase activity (pmol 3-OH-B[a]P in 1 min per 1 mg of protein) decreases in the complex form B[a]P with LP. So for transport form B[a]P with HDL the decrease was by 22%, for transport form B[a]P with VLDL the decrease was 30%, and for transport form B[a]P with LDL it was 52%. It should be noted that Km for the substrate remained practically unchanged in all forms. The Vmax and Km values indicate that the LP component in complexes with B[a]P can be a noncompetitive inhibitor of B[a]P hydroxylation in rat liver microsomes.https://ateroskleroz.elpub.ru/jour/article/view/394/374benzo[a]pyrenerat liver microsomesblood plasma lipoproteins |
| spellingShingle | L. M. Polyakov R. A. Knyazev N. V. Trifonova M. V. Kotova E. I. Solovyova A. V. Ryabchenko Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats Атеросклероз benzo[a]pyrene rat liver microsomes blood plasma lipoproteins |
| title | Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats |
| title_full | Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats |
| title_fullStr | Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats |
| title_full_unstemmed | Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats |
| title_short | Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats |
| title_sort | effect of blood plasma lipoproteins on hydroxylation of benzo a pyrene in liver microsomes of rats |
| topic | benzo[a]pyrene rat liver microsomes blood plasma lipoproteins |
| url | https://ateroskleroz.elpub.ru/jour/article/view/394/374 |
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