An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR
L-2-hydroxyglutarate (L-2-HG) is an important metabolite but its regulation is poorly understood. Here the authors report an L-2-HG FRET biosensor based on the allosteric transcription factor, LhgR, to monitor L-2-HG in cells and biological samples.
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-23723-7 |
| _version_ | 1818400654036566016 |
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| author | Zhaoqi Kang Manman Zhang Kaiyu Gao Wen Zhang Wensi Meng Yidong Liu Dan Xiao Shiting Guo Cuiqing Ma Chao Gao Ping Xu |
| author_facet | Zhaoqi Kang Manman Zhang Kaiyu Gao Wen Zhang Wensi Meng Yidong Liu Dan Xiao Shiting Guo Cuiqing Ma Chao Gao Ping Xu |
| author_sort | Zhaoqi Kang |
| collection | DOAJ |
| description | L-2-hydroxyglutarate (L-2-HG) is an important metabolite but its regulation is poorly understood. Here the authors report an L-2-HG FRET biosensor based on the allosteric transcription factor, LhgR, to monitor L-2-HG in cells and biological samples. |
| first_indexed | 2024-12-14T07:40:00Z |
| format | Article |
| id | doaj.art-f6f1f3484bad43509543b1abe04c2573 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T07:40:00Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f6f1f3484bad43509543b1abe04c25732022-12-21T23:11:05ZengNature PortfolioNature Communications2041-17232021-06-0112111310.1038/s41467-021-23723-7An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgRZhaoqi Kang0Manman Zhang1Kaiyu Gao2Wen Zhang3Wensi Meng4Yidong Liu5Dan Xiao6Shiting Guo7Cuiqing Ma8Chao Gao9Ping Xu10State Key Laboratory of Microbial Technology, Shandong UniversityTianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Department of Radiobiology, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical CollegeState Key Laboratory of Microbial Technology, Shandong UniversityCenter for Gene and Immunotherapy, The Second Hospital of Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityL-2-hydroxyglutarate (L-2-HG) is an important metabolite but its regulation is poorly understood. Here the authors report an L-2-HG FRET biosensor based on the allosteric transcription factor, LhgR, to monitor L-2-HG in cells and biological samples.https://doi.org/10.1038/s41467-021-23723-7 |
| spellingShingle | Zhaoqi Kang Manman Zhang Kaiyu Gao Wen Zhang Wensi Meng Yidong Liu Dan Xiao Shiting Guo Cuiqing Ma Chao Gao Ping Xu An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR Nature Communications |
| title | An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR |
| title_full | An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR |
| title_fullStr | An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR |
| title_full_unstemmed | An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR |
| title_short | An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR |
| title_sort | l 2 hydroxyglutarate biosensor based on specific transcriptional regulator lhgr |
| url | https://doi.org/10.1038/s41467-021-23723-7 |
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