Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments
Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed wit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867016305633 |
| _version_ | 1818821206060564480 |
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| author | Andréa A.N. Kohatsu Flávia A.J. Silva Acácio I. Francisco Aline Rimoldi Marco T.A. Silva Maria D. Vargas João A. da Rosa Regina M.B. Cicarelli |
| author_facet | Andréa A.N. Kohatsu Flávia A.J. Silva Acácio I. Francisco Aline Rimoldi Marco T.A. Silva Maria D. Vargas João A. da Rosa Regina M.B. Cicarelli |
| author_sort | Andréa A.N. Kohatsu |
| collection | DOAJ |
| description | Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. Keywords: Trypanosoma cruzi, Ferrocenyl diamine hydrochlorides, Mitochondrial tryparedoxin peroxidase (mTcTXNPx), Oxidative stress mechanisms |
| first_indexed | 2024-12-18T23:04:30Z |
| format | Article |
| id | doaj.art-f6f3766d859948049cd089b0aba7a15b |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-18T23:04:30Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-f6f3766d859948049cd089b0aba7a15b2022-12-21T20:48:29ZengElsevierBrazilian Journal of Infectious Diseases1413-86702017-03-01212125132S1413-86702017000200125Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatmentsAndréa A.N. Kohatsu0Flávia A.J. Silva1Acácio I. Francisco2Aline Rimoldi3Marco T.A. Silva4Maria D. Vargas5João A. da Rosa6Regina M.B. Cicarelli7Universidade Estadual Paulista Júlio de Mesquita Filho, Laboratório de Imunologia e Biologia Molecular, Departamento de Ciências Biológicas, Araraquara, SP, BrazilUniversidade Estadual Paulista Júlio de Mesquita Filho, Laboratório de Imunologia e Biologia Molecular, Departamento de Ciências Biológicas, Araraquara, SP, BrazilUniversidade Federal Fluminense, Instituto de Química, Niterói, RJ, BrazilUniversidade Estadual Paulista “Júlio de Mesquita Filho” Laboratório de Parasitologia, Departamento de Ciências Biológicas, Araraquara, SP, BrazilUniversidade de São Paulo, Instituto de Física, São Carlos, SP, BrazilUniversidade Federal Fluminense, Instituto de Química, Niterói, RJ, BrazilUniversidade Estadual Paulista “Júlio de Mesquita Filho” Laboratório de Parasitologia, Departamento de Ciências Biológicas, Araraquara, SP, BrazilUniversidade Estadual Paulista Júlio de Mesquita Filho, Laboratório de Imunologia e Biologia Molecular, Departamento de Ciências Biológicas, Araraquara, SP, Brazil; Corresponding author.Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. Keywords: Trypanosoma cruzi, Ferrocenyl diamine hydrochlorides, Mitochondrial tryparedoxin peroxidase (mTcTXNPx), Oxidative stress mechanismshttp://www.sciencedirect.com/science/article/pii/S1413867016305633 |
| spellingShingle | Andréa A.N. Kohatsu Flávia A.J. Silva Acácio I. Francisco Aline Rimoldi Marco T.A. Silva Maria D. Vargas João A. da Rosa Regina M.B. Cicarelli Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments Brazilian Journal of Infectious Diseases |
| title | Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| title_full | Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| title_fullStr | Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| title_full_unstemmed | Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| title_short | Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| title_sort | differential expression on mitochondrial tryparedoxin peroxidase mtctxnpx in trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
| url | http://www.sciencedirect.com/science/article/pii/S1413867016305633 |
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