Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality
Objective The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, adding an extender material to semen that inc...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2023-02-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-22-0184.pdf |
| _version_ | 1828041271835361280 |
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| author | Ragil Angga Prastiya Tri Wahyu Suprayogi Aldea Erian Debora Ani Wijayanti Anny Amalia Deny Sulistyowati Aras Prasetiyo Nugroho |
| author_facet | Ragil Angga Prastiya Tri Wahyu Suprayogi Aldea Erian Debora Ani Wijayanti Anny Amalia Deny Sulistyowati Aras Prasetiyo Nugroho |
| author_sort | Ragil Angga Prastiya |
| collection | DOAJ |
| description | Objective The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, adding an extender material to semen that increases spermatozoa’s survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation. Methods Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The samples were divided into four treatments: T0, no GTE added to the extender; T1, 0.05 mg GTE plus 100 mL extender; T2, 0.10 mg GTE plus 100 mL extender; and T3, 0.15 mg GTE plus 100 mL extender. The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and post-thawing. Results There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm. |
| first_indexed | 2024-04-10T17:07:58Z |
| format | Article |
| id | doaj.art-f6f6392c6bf14e49943c471621745d52 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-10T17:07:58Z |
| publishDate | 2023-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-f6f6392c6bf14e49943c471621745d522023-02-06T01:13:58ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352023-02-0136220921710.5713/ab.22.018424942Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm qualityRagil Angga Prastiya0Tri Wahyu Suprayogi1Aldea Erian Debora2Ani Wijayanti3Anny Amalia4Deny Sulistyowati5Aras Prasetiyo Nugroho6 Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia Singosari National Artificial Insemination Center, Directorate General of Livestock and Animal Health, Malang 65153, Indonesia Singosari National Artificial Insemination Center, Directorate General of Livestock and Animal Health, Malang 65153, Indonesia Animal Husbandry Science Department, Faculty of Animal Husbandry, Jenderal Soedirman University, Purwokerto 53122, IndonesiaObjective The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, adding an extender material to semen that increases spermatozoa’s survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation. Methods Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The samples were divided into four treatments: T0, no GTE added to the extender; T1, 0.05 mg GTE plus 100 mL extender; T2, 0.10 mg GTE plus 100 mL extender; and T3, 0.15 mg GTE plus 100 mL extender. The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and post-thawing. Results There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.http://www.animbiosci.org/upload/pdf/ab-22-0184.pdfapplied genetics and breedingbali bullcryopreservationextendergreen tea extractlivestock and gene bank |
| spellingShingle | Ragil Angga Prastiya Tri Wahyu Suprayogi Aldea Erian Debora Ani Wijayanti Anny Amalia Deny Sulistyowati Aras Prasetiyo Nugroho Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality Animal Bioscience applied genetics and breeding bali bull cryopreservation extender green tea extract livestock and gene bank |
| title | Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality |
| title_full | Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality |
| title_fullStr | Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality |
| title_full_unstemmed | Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality |
| title_short | Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality |
| title_sort | green tea extract addition into a tris based egg yolk extender improves bali bull sperm quality |
| topic | applied genetics and breeding bali bull cryopreservation extender green tea extract livestock and gene bank |
| url | http://www.animbiosci.org/upload/pdf/ab-22-0184.pdf |
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