Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors
Detection of the breast cancer cells is important for early diagnosis of the cancer. We applied thickness shear mode acoustics method (TSM) for detection of SK-BR-3 breast cancer cells using DNA aptamers specific to HER2 positive membrane receptors. The biotinylated aptamers were immobilized at the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/9/2/72 |
| _version_ | 1818862871908450304 |
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| author | Alexandra Poturnayová Ľudmila Dzubinová Monika Buríková Jozef Bízik Tibor Hianik |
| author_facet | Alexandra Poturnayová Ľudmila Dzubinová Monika Buríková Jozef Bízik Tibor Hianik |
| author_sort | Alexandra Poturnayová |
| collection | DOAJ |
| description | Detection of the breast cancer cells is important for early diagnosis of the cancer. We applied thickness shear mode acoustics method (TSM) for detection of SK-BR-3 breast cancer cells using DNA aptamers specific to HER2 positive membrane receptors. The biotinylated aptamers were immobilized at the neutravidin layer chemisorbed at gold surface of TSM transducer. Addition of the cells resulted in decrease of resonant frequency, f<sub>s</sub>, and in increase of motional resistance, R<sub>m</sub>. Using gold nanoparticles (AuNPs), modified by aptamers it was possible improving the limit of detection (LOD) that reached 550 cells/mL, while without amplification the sensitivity of the detection of SK-BR-3 cells was 1574 cells/mL. HER2 negative cell line MDA-MB-231 did not resulted in significant changes of f<sub>s</sub>. The viability studies demonstrated that cells are stable at experimental conditions used during at least 8 h. AuNPs were not toxic on the cells up to concentration of 1 μg/mL. |
| first_indexed | 2024-12-19T10:06:45Z |
| format | Article |
| id | doaj.art-f6f886f82bb9418fa143f0b2e11c72c8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-19T10:06:45Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f6f886f82bb9418fa143f0b2e11c72c82022-12-21T20:26:29ZengMDPI AGBiosensors2079-63742019-05-01927210.3390/bios9020072bios9020072Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 ReceptorsAlexandra Poturnayová0Ľudmila Dzubinová1Monika Buríková2Jozef Bízik3Tibor Hianik4Institute of Animal Biochemistry and Genetics, Center of Biosciences SAS, Dúbravská cesta 9, 840 05 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center SAS, Dúbravská cesta 9, 840 05 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center SAS, Dúbravská cesta 9, 840 05 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, SlovakiaDetection of the breast cancer cells is important for early diagnosis of the cancer. We applied thickness shear mode acoustics method (TSM) for detection of SK-BR-3 breast cancer cells using DNA aptamers specific to HER2 positive membrane receptors. The biotinylated aptamers were immobilized at the neutravidin layer chemisorbed at gold surface of TSM transducer. Addition of the cells resulted in decrease of resonant frequency, f<sub>s</sub>, and in increase of motional resistance, R<sub>m</sub>. Using gold nanoparticles (AuNPs), modified by aptamers it was possible improving the limit of detection (LOD) that reached 550 cells/mL, while without amplification the sensitivity of the detection of SK-BR-3 cells was 1574 cells/mL. HER2 negative cell line MDA-MB-231 did not resulted in significant changes of f<sub>s</sub>. The viability studies demonstrated that cells are stable at experimental conditions used during at least 8 h. AuNPs were not toxic on the cells up to concentration of 1 μg/mL.https://www.mdpi.com/2079-6374/9/2/72breast cancer cellsHER2 receptorsDNA aptameracoustic biosensorgold nanoparticles |
| spellingShingle | Alexandra Poturnayová Ľudmila Dzubinová Monika Buríková Jozef Bízik Tibor Hianik Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors Biosensors breast cancer cells HER2 receptors DNA aptamer acoustic biosensor gold nanoparticles |
| title | Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors |
| title_full | Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors |
| title_fullStr | Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors |
| title_full_unstemmed | Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors |
| title_short | Detection of Breast Cancer Cells Using Acoustics Aptasensor Specific to HER2 Receptors |
| title_sort | detection of breast cancer cells using acoustics aptasensor specific to her2 receptors |
| topic | breast cancer cells HER2 receptors DNA aptamer acoustic biosensor gold nanoparticles |
| url | https://www.mdpi.com/2079-6374/9/2/72 |
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