Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
Abstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03548-5 |
| _version_ | 1819035928959647744 |
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| author | Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier |
| author_facet | Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier |
| author_sort | Rachel Genthial |
| collection | DOAJ |
| description | Abstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however, the complexity or limitations of currently used imaging methods restrict our understanding of this functional integration. Here we address this issue using label-free third-harmonic generation (THG) microscopy. We find that the porous lacuno-canalicular network (LCN), revealing the geometry of osteocytes in the bone matrix, can be directly visualized in 3D with submicron precision over millimetric fields of view compatible with histology. THG also reveals interfaces delineating volumes formed at successive remodeling stages. Finally, we show that the structure of the LCN can be analyzed in relation with that of the extracellular matrix and larger-scale structures by simultaneously recording THG and second-harmonic generation (SHG) signals relating to the collagen organization. |
| first_indexed | 2024-12-21T07:57:26Z |
| format | Article |
| id | doaj.art-f6f8a5ad851441909b8bbcb470cd05be |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T07:57:26Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f6f8a5ad851441909b8bbcb470cd05be2022-12-21T19:10:58ZengNature PortfolioScientific Reports2045-23222017-06-017111610.1038/s41598-017-03548-5Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopyRachel Genthial0Emmanuel Beaurepaire1Marie-Claire Schanne-Klein2Françoise Peyrin3Delphine Farlay4Cécile Olivier5Yohann Bala6Georges Boivin7Jean-Claude Vial8Delphine Débarre9Aurélien Gourrier10Univ. Grenoble Alpes, LIPHYLOB, Ecole Polytechnique, CNRS, Inserm, Université Paris-SaclayLOB, Ecole Polytechnique, CNRS, Inserm, Université Paris-SaclayUniversité de Lyon, CREATIS, CNRS UMR5220, Inserm U1206, INSA-Lyon, Université Claude BernardINSERM, UMR 1033, Univ LyonUniversité de Lyon, CREATIS, CNRS UMR5220, Inserm U1206, INSA-Lyon, Université Claude BernardINSERM, UMR 1033, Univ LyonINSERM, UMR 1033, Univ LyonUniv. Grenoble Alpes, LIPHYUniv. Grenoble Alpes, LIPHYUniv. Grenoble Alpes, LIPHYAbstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however, the complexity or limitations of currently used imaging methods restrict our understanding of this functional integration. Here we address this issue using label-free third-harmonic generation (THG) microscopy. We find that the porous lacuno-canalicular network (LCN), revealing the geometry of osteocytes in the bone matrix, can be directly visualized in 3D with submicron precision over millimetric fields of view compatible with histology. THG also reveals interfaces delineating volumes formed at successive remodeling stages. Finally, we show that the structure of the LCN can be analyzed in relation with that of the extracellular matrix and larger-scale structures by simultaneously recording THG and second-harmonic generation (SHG) signals relating to the collagen organization.https://doi.org/10.1038/s41598-017-03548-5 |
| spellingShingle | Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy Scientific Reports |
| title | Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy |
| title_full | Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy |
| title_fullStr | Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy |
| title_full_unstemmed | Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy |
| title_short | Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy |
| title_sort | label free imaging of bone multiscale porosity and interfaces using third harmonic generation microscopy |
| url | https://doi.org/10.1038/s41598-017-03548-5 |
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