CCR7 defines a precursor for murine iNKT cells in thymus and periphery
The precise steps of iNKT subset differentiation in the thymus and periphery have been controversial. We demonstrate here that the small proportion of thymic iNKT and mucosal associated invariant T cells that express CCR7 represent a multi-potent progenitor pool that gives rise to effector subsets w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/34793 |
| _version_ | 1818023925520531456 |
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| author | Haiguang Wang Kristin A Hogquist |
| author_facet | Haiguang Wang Kristin A Hogquist |
| author_sort | Haiguang Wang |
| collection | DOAJ |
| description | The precise steps of iNKT subset differentiation in the thymus and periphery have been controversial. We demonstrate here that the small proportion of thymic iNKT and mucosal associated invariant T cells that express CCR7 represent a multi-potent progenitor pool that gives rise to effector subsets within the thymus. Using intra-thymic labeling, we also showed that CCR7+ iNKT cells emigrate from the thymus in a Klf2 dependent manner, and undergo further maturation after reaching the periphery. Ccr7 deficiency impaired differentiation of iNKT effector subsets and localization to the medulla. Parabiosis and intra-thymic transfer showed that thymic NKT1 and NKT17 were resident—they were not derived from and did not contribute to the peripheral pool. Finally, each thymic iNKT effector subset produces distinct factors that influence T cell development. Our findings demonstrate how the thymus is both a source of iNKT progenitors and a unique site of tissue dependent effector cell differentiation. |
| first_indexed | 2024-12-10T03:52:04Z |
| format | Article |
| id | doaj.art-f6f915ad4bed44dc823d9bf99ef84307 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:52:04Z |
| publishDate | 2018-08-01 |
| publisher | eLife Sciences Publications Ltd |
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| spelling | doaj.art-f6f915ad4bed44dc823d9bf99ef843072022-12-22T02:03:14ZengeLife Sciences Publications LtdeLife2050-084X2018-08-01710.7554/eLife.34793CCR7 defines a precursor for murine iNKT cells in thymus and peripheryHaiguang Wang0https://orcid.org/0000-0001-5403-9179Kristin A Hogquist1https://orcid.org/0000-0001-9963-5687The Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, United StatesThe Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, United StatesThe precise steps of iNKT subset differentiation in the thymus and periphery have been controversial. We demonstrate here that the small proportion of thymic iNKT and mucosal associated invariant T cells that express CCR7 represent a multi-potent progenitor pool that gives rise to effector subsets within the thymus. Using intra-thymic labeling, we also showed that CCR7+ iNKT cells emigrate from the thymus in a Klf2 dependent manner, and undergo further maturation after reaching the periphery. Ccr7 deficiency impaired differentiation of iNKT effector subsets and localization to the medulla. Parabiosis and intra-thymic transfer showed that thymic NKT1 and NKT17 were resident—they were not derived from and did not contribute to the peripheral pool. Finally, each thymic iNKT effector subset produces distinct factors that influence T cell development. Our findings demonstrate how the thymus is both a source of iNKT progenitors and a unique site of tissue dependent effector cell differentiation.https://elifesciences.org/articles/34793thymusiNKT cellsT cell development |
| spellingShingle | Haiguang Wang Kristin A Hogquist CCR7 defines a precursor for murine iNKT cells in thymus and periphery eLife thymus iNKT cells T cell development |
| title | CCR7 defines a precursor for murine iNKT cells in thymus and periphery |
| title_full | CCR7 defines a precursor for murine iNKT cells in thymus and periphery |
| title_fullStr | CCR7 defines a precursor for murine iNKT cells in thymus and periphery |
| title_full_unstemmed | CCR7 defines a precursor for murine iNKT cells in thymus and periphery |
| title_short | CCR7 defines a precursor for murine iNKT cells in thymus and periphery |
| title_sort | ccr7 defines a precursor for murine inkt cells in thymus and periphery |
| topic | thymus iNKT cells T cell development |
| url | https://elifesciences.org/articles/34793 |
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