ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein
Zika virus (ZIKV) is an RNA-enveloped virus that belongs to the <i>Flavivirus genus</i>, and ZIKV infections potentially induce severe neurodegenerative diseases and impair male fertility. Palmitoylation is an important post-translational modification of proteins that is mediated by a se...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/1/144 |
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| author | Dingwen Hu Haimei Zou Weijie Chen Yuting Li Ziqing Luo Xianyang Wang Dekuan Guo Yu Meng Feng Liao Wenbiao Wang Ying Zhu Jianguo Wu Geng Li |
| author_facet | Dingwen Hu Haimei Zou Weijie Chen Yuting Li Ziqing Luo Xianyang Wang Dekuan Guo Yu Meng Feng Liao Wenbiao Wang Ying Zhu Jianguo Wu Geng Li |
| author_sort | Dingwen Hu |
| collection | DOAJ |
| description | Zika virus (ZIKV) is an RNA-enveloped virus that belongs to the <i>Flavivirus genus</i>, and ZIKV infections potentially induce severe neurodegenerative diseases and impair male fertility. Palmitoylation is an important post-translational modification of proteins that is mediated by a series of DHHC-palmitoyl transferases, which are implicated in various biological processes and viral infections. However, it remains to be investigated whether palmitoylation regulates ZIKV infections. In this study, we initially observed that the inhibition of palmitoylation by 2-bromopalmitate (2-BP) enhanced ZIKV infections, and determined that the envelope protein of ZIKV is palmitoylated at Cys308. ZDHHC11 was identified as the predominant enzyme that interacts with the ZIKV envelope protein and catalyzes its palmitoylation. Notably, ZDHHC11 suppressed ZIKV infections in an enzymatic activity-dependent manner and ZDHHC11 knockdown promoted ZIKV infection. In conclusion, we proposed that the envelope protein of ZIKV undergoes a novel post-translational modification and identified a distinct mechanism in which ZDHHC11 suppresses ZIKV infections via palmitoylation of the ZIKV envelope protein. |
| first_indexed | 2024-03-09T11:02:43Z |
| format | Article |
| id | doaj.art-f6f96fed87e0466298b3e3a0e423ab3d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T11:02:43Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f6f96fed87e0466298b3e3a0e423ab3d2023-12-01T01:11:10ZengMDPI AGViruses1999-49152023-01-0115114410.3390/v15010144ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope ProteinDingwen Hu0Haimei Zou1Weijie Chen2Yuting Li3Ziqing Luo4Xianyang Wang5Dekuan Guo6Yu Meng7Feng Liao8Wenbiao Wang9Ying Zhu10Jianguo Wu11Geng Li12State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaThe Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, ChinaGuangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaMedical Research Center, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, ChinaState Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaGuangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, ChinaZika virus (ZIKV) is an RNA-enveloped virus that belongs to the <i>Flavivirus genus</i>, and ZIKV infections potentially induce severe neurodegenerative diseases and impair male fertility. Palmitoylation is an important post-translational modification of proteins that is mediated by a series of DHHC-palmitoyl transferases, which are implicated in various biological processes and viral infections. However, it remains to be investigated whether palmitoylation regulates ZIKV infections. In this study, we initially observed that the inhibition of palmitoylation by 2-bromopalmitate (2-BP) enhanced ZIKV infections, and determined that the envelope protein of ZIKV is palmitoylated at Cys308. ZDHHC11 was identified as the predominant enzyme that interacts with the ZIKV envelope protein and catalyzes its palmitoylation. Notably, ZDHHC11 suppressed ZIKV infections in an enzymatic activity-dependent manner and ZDHHC11 knockdown promoted ZIKV infection. In conclusion, we proposed that the envelope protein of ZIKV undergoes a novel post-translational modification and identified a distinct mechanism in which ZDHHC11 suppresses ZIKV infections via palmitoylation of the ZIKV envelope protein.https://www.mdpi.com/1999-4915/15/1/144Zika viruspalmitoylation2-BPenvelope proteinZDHHC11 |
| spellingShingle | Dingwen Hu Haimei Zou Weijie Chen Yuting Li Ziqing Luo Xianyang Wang Dekuan Guo Yu Meng Feng Liao Wenbiao Wang Ying Zhu Jianguo Wu Geng Li ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein Viruses Zika virus palmitoylation 2-BP envelope protein ZDHHC11 |
| title | ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein |
| title_full | ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein |
| title_fullStr | ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein |
| title_full_unstemmed | ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein |
| title_short | ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein |
| title_sort | zdhhc11 suppresses zika virus infections by palmitoylating the envelope protein |
| topic | Zika virus palmitoylation 2-BP envelope protein ZDHHC11 |
| url | https://www.mdpi.com/1999-4915/15/1/144 |
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