A thermophilic L-lactic acid producer of high optical purity: isolation and identification
Biodegradable polymers, specifically polylactide, are an important part of food packaging and medical devices. Microbiological synthesis uses cheap renewable raw materials and industrial waste to produce a high yield of lactic acid, the monomer of polylactide. This method needs new effective lacti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kemerovo State University
2024-01-01
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| Series: | Foods and Raw Materials |
| Subjects: | |
| Online Access: | https://jfrm.ru/en/issues/21683/21698/ |
| _version_ | 1797301361627365376 |
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| author | Mariia V. Romanova Anastasiia N. Dolbunova Yulia M. Epishkina Svetlana A. Evdokimova Mikhail R. Kozlovskiy Alexander Ye. Kuznetsov Natalya Yu. Khromova Andrey V. Beloded |
| author_facet | Mariia V. Romanova Anastasiia N. Dolbunova Yulia M. Epishkina Svetlana A. Evdokimova Mikhail R. Kozlovskiy Alexander Ye. Kuznetsov Natalya Yu. Khromova Andrey V. Beloded |
| author_sort | Mariia V. Romanova |
| collection | DOAJ |
| description | Biodegradable polymers, specifically polylactide, are an important part of food packaging and medical devices. Microbiological
synthesis uses cheap renewable raw materials and industrial waste to produce a high yield of lactic acid, the monomer of
polylactide. This method needs new effective lactic acid producing strains, e.g., thermophilic bacteria.
The research involved thermophilic bacterial strains isolated from soil and compost samples. Their ability to produce organic
acids and extracellular enzymes was tested using the method of high-performance liquid chromatography (HPLC) and
microbiological tests respectively. The real-time polymerase chain reaction method (PCR) detected L-lactate dehydrogenase
structural genes of L-lactate dehydrogenase of Bacillaceae. Strain T7.1 was fermented using glucose and yeast extract as carbon
and nitrogen sources, respectively. The optical purity of lactic acid was evaluated using quantitative gas chromatography on a
chiral column to separate lactate isomers. The molecular genetic analysis of the 16S rRNA gene sequence was applied to identify
strain T7.1.
The chromatographic analysis proved that 10 out of 13 isolated thermophilic strains were effective lactic acid producers. They
demonstrated proteolytic, amylolytic, or cellulase activities. During the fermentation, strain T7.1 produced 81 g/L of lactic
acid with a peak productivity at 1.58 g/(L·h). The optical purity of the product exceeded 99.9% L-lactate. The genetic analysis
identified strain T7.1 as Weizmannia coagulans (Bacillus coagulans).
The research revealed a promising thermophilic producer of optically pure L-lactic acid. Further research is needed to optimize
the cultivation conditions, design an effective and cheap nutrient medium, and develop engineering and technological solutions to
increase the yield. |
| first_indexed | 2024-03-07T23:21:26Z |
| format | Article |
| id | doaj.art-f6fa31fd2c944e1d93bd335be2699916 |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-03-07T23:21:26Z |
| publishDate | 2024-01-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-f6fa31fd2c944e1d93bd335be26999162024-02-21T05:52:19ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992024-01-0112110110910.21603/2308-4057-2024-1-591A thermophilic L-lactic acid producer of high optical purity: isolation and identificationMariia V. Romanova0https://orcid.org/0000-0003-3109-8445Anastasiia N. Dolbunova1https://orcid.org/0009-0003-7884-3198Yulia M. Epishkina2https://orcid.org/0000-0002-7776-2922Svetlana A. Evdokimova3https://orcid.org/0000-0002-4808-5002Mikhail R. Kozlovskiy4https://orcid.org/0000-0003-0774-2886Alexander Ye. Kuznetsov5https://orcid.org/0000-0003-2574-2118Natalya Yu. Khromova6https://orcid.org/0000-0001-9852-9401 Andrey V. Beloded7https://orcid.org/0000-0002-4425-8068Dmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaDmitry Mendeleev University of Chemical Technology of Russia, Moscow, RussiaBiodegradable polymers, specifically polylactide, are an important part of food packaging and medical devices. Microbiological synthesis uses cheap renewable raw materials and industrial waste to produce a high yield of lactic acid, the monomer of polylactide. This method needs new effective lactic acid producing strains, e.g., thermophilic bacteria. The research involved thermophilic bacterial strains isolated from soil and compost samples. Their ability to produce organic acids and extracellular enzymes was tested using the method of high-performance liquid chromatography (HPLC) and microbiological tests respectively. The real-time polymerase chain reaction method (PCR) detected L-lactate dehydrogenase structural genes of L-lactate dehydrogenase of Bacillaceae. Strain T7.1 was fermented using glucose and yeast extract as carbon and nitrogen sources, respectively. The optical purity of lactic acid was evaluated using quantitative gas chromatography on a chiral column to separate lactate isomers. The molecular genetic analysis of the 16S rRNA gene sequence was applied to identify strain T7.1. The chromatographic analysis proved that 10 out of 13 isolated thermophilic strains were effective lactic acid producers. They demonstrated proteolytic, amylolytic, or cellulase activities. During the fermentation, strain T7.1 produced 81 g/L of lactic acid with a peak productivity at 1.58 g/(L·h). The optical purity of the product exceeded 99.9% L-lactate. The genetic analysis identified strain T7.1 as Weizmannia coagulans (Bacillus coagulans). The research revealed a promising thermophilic producer of optically pure L-lactic acid. Further research is needed to optimize the cultivation conditions, design an effective and cheap nutrient medium, and develop engineering and technological solutions to increase the yield.https://jfrm.ru/en/issues/21683/21698/thermophilic bacteriabacillusweizmannia coagulanslactic acidl-lactatepolylactide |
| spellingShingle | Mariia V. Romanova Anastasiia N. Dolbunova Yulia M. Epishkina Svetlana A. Evdokimova Mikhail R. Kozlovskiy Alexander Ye. Kuznetsov Natalya Yu. Khromova Andrey V. Beloded A thermophilic L-lactic acid producer of high optical purity: isolation and identification Foods and Raw Materials thermophilic bacteria bacillus weizmannia coagulans lactic acid l-lactate polylactide |
| title | A thermophilic L-lactic acid producer of high optical purity: isolation and identification |
| title_full | A thermophilic L-lactic acid producer of high optical purity: isolation and identification |
| title_fullStr | A thermophilic L-lactic acid producer of high optical purity: isolation and identification |
| title_full_unstemmed | A thermophilic L-lactic acid producer of high optical purity: isolation and identification |
| title_short | A thermophilic L-lactic acid producer of high optical purity: isolation and identification |
| title_sort | thermophilic l lactic acid producer of high optical purity isolation and identification |
| topic | thermophilic bacteria bacillus weizmannia coagulans lactic acid l-lactate polylactide |
| url | https://jfrm.ru/en/issues/21683/21698/ |
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