Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer
BackgroundHypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action are reduced by the hypoxia-inducible factor 1-alpha subunit suppressor (HIF1AN). Its inappropriate expression is associated with the development of cancer and immune control. It is yet unknown how HIF1AN, clinic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1080910/full |
| _version_ | 1797934933977595904 |
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| author | Shasha Tang Dongyang Liu Yuan Fang Liyun Yong Yi Zhang Mengying Guan Xiaoyan Lin Hui Wang Fengfeng Cai |
| author_facet | Shasha Tang Dongyang Liu Yuan Fang Liyun Yong Yi Zhang Mengying Guan Xiaoyan Lin Hui Wang Fengfeng Cai |
| author_sort | Shasha Tang |
| collection | DOAJ |
| description | BackgroundHypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action are reduced by the hypoxia-inducible factor 1-alpha subunit suppressor (HIF1AN). Its inappropriate expression is associated with the development of cancer and immune control. It is yet unknown how HIF1AN, clinical outcomes, and immune involvement in breast cancer (BC) are related.MethodsUsing the GEPIA, UALCAN, TIMER, Kaplan-Meier plotter, and TISIDB datasets, a thorough analysis of HIF1AN differential expression, medical prognosis, and the relationship between HIF1AN and tumor-infiltrating immune cells in BC was conducted. Quantitative real-time PCR (qRT−PCR) analysis of BC cells were used for external validation.ResultsThe findings revealed that, as compared to standard specimens, BC cells had significantly lower levels of HIF1AN expression. Good overall survival (OS) for BC was associated with higher HIF1AN expression. Additionally, in BC, the expression of HIF1AN was closely associated with the chemokines and immune cell infiltration, including neutrophils, macrophages, T helper cells, B cells, Tregs, monocytes, dendritic cells, and NK cells. A high correlation between HIF1AN expression and several immunological indicators of T-cell exhaustion was particularly revealed by the bioinformatic study.ConclusionsHIF1AN is a predictive indicator for breast tumors, and it is useful for predicting survival rates. |
| first_indexed | 2024-04-10T18:07:45Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T18:07:45Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-f6fabb9fd9ce44c0843f200d6100d5202023-02-02T12:45:51ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-02-011310.3389/fonc.2023.10809101080910Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancerShasha Tang0Dongyang Liu1Yuan Fang2Liyun Yong3Yi Zhang4Mengying Guan5Xiaoyan Lin6Hui Wang7Fengfeng Cai8Department of Breast Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Breast Surgery, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, ChinaLaboratory of Tumor Molecular Biology, School of Basic Medical Sciences, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaDepartment of Breast Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaBackgroundHypoxia-inducible factor 1-alpha (HIF-1α) stability and transcriptional action are reduced by the hypoxia-inducible factor 1-alpha subunit suppressor (HIF1AN). Its inappropriate expression is associated with the development of cancer and immune control. It is yet unknown how HIF1AN, clinical outcomes, and immune involvement in breast cancer (BC) are related.MethodsUsing the GEPIA, UALCAN, TIMER, Kaplan-Meier plotter, and TISIDB datasets, a thorough analysis of HIF1AN differential expression, medical prognosis, and the relationship between HIF1AN and tumor-infiltrating immune cells in BC was conducted. Quantitative real-time PCR (qRT−PCR) analysis of BC cells were used for external validation.ResultsThe findings revealed that, as compared to standard specimens, BC cells had significantly lower levels of HIF1AN expression. Good overall survival (OS) for BC was associated with higher HIF1AN expression. Additionally, in BC, the expression of HIF1AN was closely associated with the chemokines and immune cell infiltration, including neutrophils, macrophages, T helper cells, B cells, Tregs, monocytes, dendritic cells, and NK cells. A high correlation between HIF1AN expression and several immunological indicators of T-cell exhaustion was particularly revealed by the bioinformatic study.ConclusionsHIF1AN is a predictive indicator for breast tumors, and it is useful for predicting survival rates.https://www.frontiersin.org/articles/10.3389/fonc.2023.1080910/fullbreast cancerhypoxia-inducible factor 1-alpha subunit inhibitor (HIF1AN)prognosisimmune infiltrating cellbiomarker |
| spellingShingle | Shasha Tang Dongyang Liu Yuan Fang Liyun Yong Yi Zhang Mengying Guan Xiaoyan Lin Hui Wang Fengfeng Cai Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer Frontiers in Oncology breast cancer hypoxia-inducible factor 1-alpha subunit inhibitor (HIF1AN) prognosis immune infiltrating cell biomarker |
| title | Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| title_full | Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| title_fullStr | Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| title_full_unstemmed | Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| title_short | Low expression of HIF1AN accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| title_sort | low expression of hif1an accompanied by less immune infiltration is associated with poor prognosis in breast cancer |
| topic | breast cancer hypoxia-inducible factor 1-alpha subunit inhibitor (HIF1AN) prognosis immune infiltrating cell biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1080910/full |
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